Simultaneous determination of thirteen components of Moutan Cortex in different regions by HPLC

XIA Cheng-kai1,2 ZHAN Yun-wu3 HU Yun-fei4 FANG Cheng-wu3

(1.Bozhou Institute of Chinese Medicine, Anhui Academy of Chinese Medicine, Bozhou, China 236800)
(2.Bozhou Vocational and Technical College, Bozhou, China 236800)
(3.Anhui University of Chinese Medicine, Hefei, China 230031)
(4.Hefei Food and Drug Inspection Institute, Hefei, China 230031)

【Abstract】 Objective To establish an HPLC method for the analysis of thirteen main chemical constituents (gallicacid, 5-hydroxymethylfurfural, methylgallate, oxypaeoniflora, catechins, paeoniflorin, 1,2,3,6-tetra-O-galloyl-D-glucose, 1,2,4,6-tetra-O-galloyl-D-glucose, benzoicacid, 1,2,3,4,6-penta-O-galloyl-D-glucose, mudanpioside C, benzoyloxypaeoniflorin, and paeonal) in Moutan Cortex from different origins. Methods The methanol (50%) reflux extraction was adopted to Moutan Cortex. Separation was carried out on C18 (250 mm × 4.6 mm, 5 μm), the mobile phase was eluted with acetonitrile-0.5% phosphoric acid. The flow rate was 1.0 mL/min, the detection wavelength was 254 nm, and the column temperature was 30 °C. Results The linear relation of thirteen main active components measured in the range of mass concentration was good with perfect precision, repeatability, and stability, the value of which was all more than 0.999 5. Conclusion The HPLC method established for simultaneous determination of thirteen main chemical compositions in Moutan Cortex is effective, accurate, and reproducible, which can be used for the quality control of Moutan Cortex.

【Keywords】 Moutan Cortex; HPLC; gallic acid; 5-hydroxymethylfurfural; methylgallate; oxypaeoniflora; catechins; paeoniflorin; 1,2,3,6-tetra-O-galloyl-D-glucose; 1,2,4,6-tetra-O-galloyl-D-glucose; benzoic acid; 1,2,3,4,6-penta-O-galloyl-D-glucose; mudanpioside C; benzoyloxypaeoniflorin; paeonal;


【Funds】 Anhui Provincial Science and Technology Major Project (1603081113) Natural Science Foundation Key Project of the Education Department of Anhui Province (KJ2015A388) Project of Chinese Medicine Standardization of National Administration of Traditional Chinese Medicine (Official Letter on Science and Technology of National Administration of Traditional Chinese Medicine of China No. 2016) Action Plan of Leading Talents in Innovation and Entrepreneurship in Bozhou [Bozhou Organization Department No. 2016 (32)] Anhui Provincial Agricultural Science and Technology Innovation Fund [Secretary Department of Science and Agriculture No. 2017 (344)]

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(Translated by ZHAO B)


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This Article



Vol 50, No. 04, Pages 970-974

February 2019


Article Outline


  • 1 Instruments and materials
  • 2 Methods and results
  • 3 Discussion
  • References