Protective effect and mechanism of total flavonoids of Fructus Livistonae on acetaminophen-induced liver injury

LUO Xiao-yun1,2 MAI Yan-sui1,2 ZHU Li1,2 ZHANG Xiao-jun1 HUANG Song1,3,2 LAI Xiao-ping1,3,2

(1.Guangzhou University of Chinese Medicine, Guangzhou, China 510006)
(2.Guangdong Provincial Key Laboratory of New Chinese Medicine Research and Development, Guangzhou, China 510006)
(3.Dongguan Institution for Mathematics and Theoretics Engineering Research of Guangzhou University of Chinese Medicine, Dongguan, China 523808)

【Abstract】 Objective To research the effects of total flavonoids of Livistona chinensis (TFFL) on normal liver LO2 cells induced by acetaminophen (APAP), and study the liver protective mechanism on liver injury induced by acetaminophen in vitro. Methods The effects of TFFL on LO2 cells and cell activity induced by acetaminophen were determined by MTT method; The apoptosis rate of LO2 cells induced by APAP was researched by Flow Cytometry combine with staining agent Hoechst 33342; The levels of malondialdehyde (MDA), aspartate aminotransferase (AST), reduced glutathione (GSH), and superoxide dismutase (SOD) were examined; The expressions of iNOS and nitrotyrosine tubulin (NT) in APAP-induced LO2 hepatocyte injury cells was studied by Western blotting method. Results TFFL had no toxicity to LO2 cells at a given concentration which can promote the proliferation. At the same time, experiment results showed that TFFL prophylactically reduced the apoptosis of LO2 cells induced by APAP. TFFL decreased the level of AST and MDA, increased the level of GSH and SOD, and inhibited the expressions of iNOS protein and NT protein. Conclusion TFFL has a protective effect on LO2 cells injury induced by APAP. The possible protective mechanism of TFFL is related to the inhibition of oxidative stress and the nitro stress.

【Keywords】 Fructus Livistonae; total flavonoids; acetaminophen; liver injury; cell injury;

【DOI】

【Funds】 Major Science and Technology Planning Project of Guangdong Province (2013A022100002)

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(Translated by GENG S)

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This Article

ISSN:0253-2670

CN:12-1108/R

Vol 50, No. 04, Pages 925-930

February 2019

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Article Outline

Abstract

  • 1 Materials
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References