Protective effect and mechanism of total flavonoids of Fructus Livistonae on acetaminophen-induced liver injury
(2.Guangdong Provincial Key Laboratory of New Chinese Medicine Research and Development, Guangzhou, China 510006)
(3.Dongguan Institution for Mathematics and Theoretics Engineering Research of Guangzhou University of Chinese Medicine, Dongguan, China 523808)
【Abstract】 Objective To research the effects of total flavonoids of Livistona chinensis (TFFL) on normal liver LO2 cells induced by acetaminophen (APAP), and study the liver protective mechanism on liver injury induced by acetaminophen in vitro. Methods The effects of TFFL on LO2 cells and cell activity induced by acetaminophen were determined by MTT method; The apoptosis rate of LO2 cells induced by APAP was researched by Flow Cytometry combine with staining agent Hoechst 33342; The levels of malondialdehyde (MDA), aspartate aminotransferase (AST), reduced glutathione (GSH), and superoxide dismutase (SOD) were examined; The expressions of iNOS and nitrotyrosine tubulin (NT) in APAP-induced LO2 hepatocyte injury cells was studied by Western blotting method. Results TFFL had no toxicity to LO2 cells at a given concentration which can promote the proliferation. At the same time, experiment results showed that TFFL prophylactically reduced the apoptosis of LO2 cells induced by APAP. TFFL decreased the level of AST and MDA, increased the level of GSH and SOD, and inhibited the expressions of iNOS protein and NT protein. Conclusion TFFL has a protective effect on LO2 cells injury induced by APAP. The possible protective mechanism of TFFL is related to the inhibition of oxidative stress and the nitro stress.
【Keywords】 Fructus Livistonae; total flavonoids; acetaminophen; liver injury; cell injury;
(Translated by GENG S)
 Sun X M, Yan S, Tian S X. 中药治疗肝损伤的研究进展 [J]. Journal of Chinese Medicinal Materials, 2016, 5 (11): 2664–2667 (in Chinese).
 Chen Y, Yao H, Li S G, et al. HPLC determination of the six components in Livistona chinensis [J]. Chinese Journal of Pharmaceutical Analysis, 2011, 31 (4): 645–650 (in Chinese).
 Chen Y, Lin X H. Anti-tumor activity of the extracts of Livistona chinesis in vitro [J]. Journal of Fujian Medical University, 2008, 42 (2): 93–95 (in Chinese).
 Zhang L. Study on the liver protection and anti-oxidation activity of Fructus Livistonae (蒲葵子抗氧化及保肝活性研究) [D]. Guangzhou: Guangzhou University of Chinese Medicine, 2016 (in Chinese).
 Uzi D, Barda L, Scaiewicz V, et al. CHOP is a critical regulator of acetaminophen-induced hepatotoxicity [J]. JHepatol, 2013, 3 (11): 1118–1129.
 Liu M W, Liu R, Wu H Y, et al. Protective effect of Xuebijing injection on D-galactosamine-and lipopolysaccharide-induced acute liver injury in rats through the regulation of p38 MAPK, MMP-9 and HO-1expression by increasing TIPE2 expression [J]. Int J Mol Med, 2016, 38 (5): 1419–1432.
 Liao W Q, Qi Y L, Wang L, et al. Recql5 protects against lipopolysaccharide/D-galactosamine-induced liver injury in mice [J]. World J Gastroenterol, 2015, 21 (36): 176–180.
 Chen Y H, Chen H L, Chien C S, et al. Contribution of mature hepatocytes to biliary regeneration in rats with acute and chronic biliary injury [J]. PLoS One, 2015, 10 (8): 543–561.
 Zhang W, Yin L, Tao X, et al. Dioscin alleviates dimethylnitrosamine-induced acute liver injury through regulating apoptosis, oxidative stress and inflammation [J]. Environ Toxicol Pharmacol, 2016, 10 (6): 976–997.
 Liu L, Xiong C J, Zhu Y L, et al. The separation and anticancer activity of active monomers from sabal palm seeds [J]. Journal of Beijing University of Chemical Technology (Natural Science Edition), 2015, 4 (6): 78–83 (in Chinese).
 Zhang D M, Huang X, Yan C L, et al. 实验性肝损伤模型的研究进展 [J]. Chinese Journal of Hospital Pharmacy, 2013, 33 (22): 1871–1873 (in Chinese).
 Nelson S D. Mechanisms of the formation and disposition of reactive metabolites that can cause acute liver injury [J]. Drug Metab Rev, 1995, 27 (1/2): 147–177.
 Pumford N R, Halmes N C, Hinson J A. Covalent binding of xenobiotics to specific proteins in the liver [J]. Drug Metab Rev, 1997, 10 (5): 6675–6683.