Optimization of Yi medicine processing technology for honeyed wine rhubarb by Box-behnken design response surface methodology

WANG Yue1 LV Lu-yang1 LI Ying1 HE Jian-ping2 XIAO Ru-ming3 YANG Kai1 MA Zi-hao1 ZENG Rui1

(1.School of Pharmacy, Southwest Minzu University, Chengdu, China 610041)
(2.Chinese Medicine Hospital of Chuxiong Prefecture, Chuxiong, China 675000)
(3.Science, Technology, and Intellectual Property Office of Heishui County, Heishui, China 623500)

【Abstract】 Objective To optimize the processing technology of honeyed wine rhubarb by Box-behnken design response surface methodology. Methods Taking the content measurement combined with the sensory score as the index, the factors of particle size, steaming time, storage temperature, and moisture content were investigated by single factor tests. On the basis of the results of single factor experiments, the free and total anthraquinones contents and the extract content in honeyed wine rhubarb were determined by HPLC method and hot-dip method, respectively. Three factors (steaming time, storage temperature, and the moisture content) were investigated further by response surface, the processing technology of honeyed wine rhubarb was optimized by response surface methodology. Results The optimal parameters of processing technology were as follows: particle size was the powder through 100 mesh sieves, steaming time was 3.5 h, storage temperature was 33 ℃, and moisture content was 43%. Conclusion The optimized processing technology is simple and feasible, which can provide a reference for the processing of honeyed wine rhubarb.

【Keywords】 Radix et Rhizoma Rhei; honeyed wine rhubarb; processing technology; Box-Behnken design; response surface methodology; HPLC;


【Funds】 National Key R&D Program of China (2017YFC1700705) Sichuan Provincial Funding Scheme to Outstanding Scientific and Technological Programs by Chinese Students Abroad (2018-68) Sichuan Provincial Key Basic Research Project (2018JY0069) Southwest Minzu University 2018 Fundamental Research Funds for the Central Universities (2018NZD18) Special Fund for Poverty Reduction in Sichuan Province (2016NZYZF0015) Southwest Minzu University Graduate Innovative Research Project (CX2017SZ094) Undergraduate Innovation Project (X201810656213)

Download this article


    [1] Chinese Pharmacopoeia [S]. Volume I. 2015 (in Chinese).

    [2] Li P, Lu Q, Jiang W, et al. Pharmacokinetics and pharmacodynamics of rhubarb anthraquinones extract in normal and disease rats [J]. Biomed Phaemacother, 2017, 91: 425–435.

    [3] Wu W J, Yan R, Li T, et al. Pharmacokinetic alterations of rhubarb anthraquinones in experimental colitis induced by dextran sulfate sodium in the rat [J]. J Ethnophrmacol, 2017, 198: 600–607.

    [4] Yang Z, Fan R, Sun P, et al. Rhubarb attenuates cerebral edema via inhibition of the extracellular signal-regulated kinase pathway following traumatic brain injury in rats [J]. Pharmacogn Mag, 2018, 14 (53): 134–139.

    [5] Neyrinck A M, Etxeberria U, Taminiau B, et al. Rhubarb extract prevents hepatic inflammation induced by acute alcohol intake, an effect related to the modulation of the gut microbiota [J]. Mol Nutr Food Res, 2017, 61 (1): 1–12.

    [6] Zheng Q X, Wu H F, Guo J, et al. Review of rhubarbs: Chemistry and pharmacology [J]. Chin Herb Med, 2013, 5 (1): 9–32.

    [7] Tan P, Zhang H Z, Li Y et al. Preliminary study on antiplatelet aggregation of 10 anthraquinones in Rhei Radix et Rhizoma based on bioassay [J]. Chin Tradit Herb Drugs, 2018, 49 (4): 859–865 (in Chinese).

    [8] Xiong W Y, Zheng Y, Lu L X, et al. Research progress on protective mechanism of emodin on gastrointestinal mucosal injury [J]. Drugs Clin, 2018, 33 (9): 2 466–2 470 (in Chinese).

    [9] Bu T C, Liu Z H, Liu K X. Progress in effects of rhein on drug transporters and metabolic enzymes [J]. Drug Eval Res, 2017, 40 (9): 1 223–1 228 (in Chinese).

    [10] Gong Y, Jin Q X, Bai J Y, et al. Effect of Rheum officinale on activity of intestinal stem cells in Drosophila melanogaster induced by SDS toxicity [J]. Chin Tradit Herb Drugs, 2017, 48 (12): 2 466–2 473 (in Chinese).

    [11] Lu Q F, Li P J, Jiang W J, et al. Effects of rhein on metabolism and excretion in rat ig high-dose paracetamol [J]. Drugs Clin, 2017, 32 (3): 370–375 (in Chinese).

    [12] Xiong S M, Zhang J X, Kang W, et al. Emodin induces mitochondrial apoptosis in human hepatoma HepG2 cells [J]. Drug Eval Res, 2018, 41 (5): 773–779 (in Chinese).

    [13] Li G K, He Y C. Yi Medical botanicals [M]. Chengdu: Sichuan Nationality Press, 1990 (in Chinese).

    [14] Wang H Y. Effect of emodin on CT26 colon cancer mice [J]. Chin J Clin Pharm, 2018, 34 (12): 1 463–1 469 (in Chinese).

    [15] Yunnan Yi Hospital. Yunnan Yi therapeutics and medicines·Yunnan Yi medicines (Volume II) [M]. Kunming: Yunnan Science and Technology Press, 2007 (in Chinese).

    [16] He J P. Yi medicine concocted features [J]. China Prescr Drug, 2017, 15 (1): 19–21 (in Chinese).

    [17] Tian H Y, Qu X Y, Xiong P H. Collection of Chinese ethnic medicine processing methods [M]. Beijing: Beijing Traditional Chinese Medicine Publishing House, 2000 (in Chinese).

    [18] Ye D J, Zhang S C, Wu H. Traditional Chinese medicine processing [M]. Beijing: People's Medical Publishing House, 2011 (in Chinese).

    [19] Liu C X, Chen S L, Xiao X H, et al. A new concept on quality marker of Chinese Materia Medica: Quality control for Chinese medicinal products [J]. Chin Tradit Herb Drugs, 2016, 47 (9): 1 443–1 457 (in Chinese).

    [20] Xu Y L, Wu L H, Li P C et al. Quality evaluation for Citri Reticulatae Pericarpium Viride based on clinically utilized quantity of hesperidin [J]. Chin Tradit Herb Drugs, 2016, 47 (22): 4 009–4 015 (in Chinese).

    [21] Feng S X, Li M M, Zhao D, et al. Simultaneous determination of 10 anthraquinones in rhubarb based on HPLC-Q-HR/MS [J]. Chin Herb Med, 2017, 9 (4): 388–395.

    [22] Tan P, Zhang H Z, Zhang Q et al. Simultaneous determination of 10 anthraquinone derivatives in Rhei Radix et Rhizoma based on UPLC [J]. Chin Tradit Herb Drugs, 2018, 49 (4): 928–934 (in Chinese).

This Article



Vol 50, No. 04, Pages 844-851

February 2019


Article Outline


  • 1 Instruments and materials
  • 2 Methods and results
  • 3 Discussion
  • References