Imprinting equivalence research of Lonicerae Japonicae Flos and Lonicerae Flos

LIU Hui1,2,3 HE Hong1,2,3,4 ZHU Zhi-Fei1,2,3 FAN Qi-Meng1,2,3 ZHOU Jin1,2,3 HE Fu-yuan1,2,3

(1.College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan Province, China 410208)
(2.Hunan Provincial Key Laboratory of Drugability and Preparation Modification of TCM, Changsha, Hunan Province, China 410208)
(3.Laboratory of Supramolecular Mechanism and Mathematic-Physics Characterization for Chinese Materia Medica, Hunan University of Chinese Medicine, Changsha, Hunan Province, China 410208)
(4.Department of Pharmacy, Maternal and Child Health Hospital of Changsha County, Changsha, Hunan Province, China 410208)

【Abstract】Objective To explore and confirm the imprinting equivalence of Lonicerae Japonicae Flos (LJF) and Lonicerae Flos (LF) based on the autonomous function of the supramolecular imprinted template, so as to provide a new solution for double followers dispute. Methods With LJF dregs, LF dregs, and Chrysanthemi Flos (CF) dregs as host molecules, and the water extracts of LJF, LF, Shuanghuanglian (LJF), Shuanghuanglian (LF), Yinqiaosan (LJF), and Yinqiaosan (LF) as guest molecules, the selective absorption of three host molecules and six guest molecules was carried out. The changes in the fingerprints of water extracts of guest molecules were determinated by HPLC. Then, the MRT and their difference value were calculated through the total quantum statistical moment method, and t-test was performed on it. Results When the six guest molecules were absorbed by LJF and LF dregs, LJF and CF dregs, LF and CF dregs, the MRT difference value was conducted by t-test. The results were P1 = 0.94 > 0.05, P2 = 0.02 < 0.05, and P3 = 0.04 < 0.05. We could see that when all the guest molecules were absorbed by LJF and LF dregs, there was no significant difference in the MRT difference value. However, when the six guest molecules were absorbed by CF dregs, there were significant differences as compared with those by LJF and CF dregs. Conclusion The statistical data have revealed the similarity of imprinting templates between LJF and LF, but their imprinting templates were different from that of CF, proving the imprinting equivalence between LJF and LF, which was also in consistence with the clinical medication.

【Keywords】 Lonicerae Japonicae Flos; Lonicerae Flos; Chrysanthemi Flos; supramolecular; imprinted template; heterogeneous equivalence; total quantum statistical moment; Shuanghuanglian; Yinqiaosan;

【DOI】

【Funds】 National Natural Science Foundation of China (81573691) National Natural Science Foundation of China (81703824) National Natural Science Foundation of China (81874507) National Natural Science Foundation of China (81803729) National Natural Science Foundation of China (81874344) Hunan Provincial Natural Science Foundation of China (2017JJ3236) Hunan Provincial Natural Science Foundation of China (2016JJ4065) Scientific Research Fund of Hunan Provincial Education Department of China (17B200) Open Fund of Pharmacy in Hunan Province for “Cultivation of First-Class Discipline in China" Open Fund for the Hunan Provincial Key Laboratory of Drugability and Preparation Modification of TCM Fund by 2011 Collaborative Innovation Center Fund by China Scholarship Council

Download this article

(Translated by ZHAO B)

    References

    [1] Lu J R, Yang J Y. Review of studies on Lonicerae Japonicae Flos [J]. Chinese Medicine Modern Distance Education of China, 2012, 10 (18): 112–115 (in Chinese).

    [2] Wu F Y, Feng S G, Zeng J G. Identification and attribution of Lonicerae Japonicae Flos and Lonicera Flos [J]. Chinese Traditional and Herbal Drugs, 2014, 45(8): 1150–1156 (in Chinese).

    [3] Wang Z P, Deng J G, Wang Q et al. Recent advances in the study of LoniceraeFlos [J]. Journal of Guangxi Traditional Chinese Medical University, 2008, 11 (4): 59–61 (in Chinese).

    [4] Wang Y P, Xue X Y, Zhang F F et al. Separation and analysis of volatile off from Lonicerajaponica thunb using normal phase liquid chromatography-gas chromatography hyphenated with mass spectrometry [J]. Modernization of Traditional Chinese Medicine and Materia Materia-World Science and Technology, 2008, 10 (6): 45–55 (in Chinese).

    [5] Ni F Y, Wen J H, Li M et al. Chemical constituents from flower buds of Lonicerajaponica [J]. Chinese Traditional and Herbal Drugs, 2017, 48(18): 3689–3692 (in Chinese).

    [6] Xia Y, Li D Z, Pei Z Z, et al. Review on the chemical constituents of the flower buds of Lonicerajaponica [J]. Modern Chinese Medicine, 2012, 14 (4): 26–32 (in Chinese).

    [7] He Q H, Tian Y Y, Li H J et al. Study on iridoid glucosides in stem of Lonicera hypoglauca [J]. Chinese Pharmaceutical Journal, 2006, 41 (9): 656–658 (in Chinese).

    [8] Liu Y N, Shi D H, Lei L C. HPLC fingerprint identification of Lonicera Japonica Flos and Lonicera Flos [J]. Chinese Traditional and Herbal Drugs, 2017, 48 (4): 773–776 (in Chinese).

    [9] Zhang B X, Zhou F Q, Guo Q M. Research progress of flavonoids on LoniceraejaponicaeFlos in last five years [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18 (23): 349–352 (in Chinese).

    [10] Lee E J, Kim J S, Kim H P. Phenolic constituents from the flower buds of Lonicera japonica and their 5-lipoxygenase inhibitory activities [J]. Food Chem, 2010, 120 (1) : 134-139.

    [11] Wang H B, Deng L, Ma Y C, et al. Study on identification and determination of Lonicerae Japonicae Flos and Lonicerae Flos by UHPLC [J]. Chinese Traditional and Herbal Drugs, 2017, 48 (12): 2516–2521 (in Chinese).

    [12] Chai X Y, Wang L, Song Y, et al. Study on the flavonoids from Loniceraconfusa DC. [J]. Journal of China Pharmaceutical University, 2004, 35 (4): 9–12 (in Chinese).

    [13] Chai X Y, Li P, Dou J, et al. Studies on the saponins from Loniceraconfusa DC. [J]. Chinese Journal of Natural Medicines, 2004, 2 (2): 22–26 (in Chinese).

    [14] Wen J H, Ni F Y, Zhao Y W et al. Chemical constituents from flower buds of Lonicerae Flos [J]. Chinese Traditional and Herbal Drugs, 2015, 46 (13): 1883–1886 (in Chinese).

    [15] Chai X Y, Dou J, He Q H et al. Studies on the phenolic acid compounds from Loniceraconfusa DC. [J]. Chinese Journal of Natural Medicines, 2004, 2 (6): 339 (in Chinese).

    [16] Xiao M F, Liu F L, Zhou J et al. Research status of Lonicera Japonicae Flos and Lonicerae Flos and its key issues for quality control [J]. Chinese Traditional and Herbal Drugs, 2018, 49 (20): 4905–4911 (in Chinese).

    [17] Liu J K. Lonicerae Japonicae Flos and Lonicerae Flos are the same or not [N]. China Pharmaceutical News, March 24, 2014 (008) (in Chinese).

    [18] Feng B B, Wang X C, Zhang J H et al. Comparative study on the content of chiorogenic acid in Lonicerae Japonicae Flos and Lonicerae Flos from different producing areas [J]. Journal of Anhui Agricultural Sciences, 2012, 40 (2): 729–730 (in Chinese).

    [19] Yang Q R, Zhao Y Y, Hao J B, et al. Research progress on chemical constituents and their differences between Lonicerae Japonicae Flos and Lonicerae Flos [J]. China Journal of Chinese Materia Medica, 2016, 41(7): 1204–1211 (in Chinese).

    [20] Bao G Z. Characters identification and difference in the pharmacological effects of Lonicerae Japonicae Flos and LoniceraeFlos [J]. Journal of North Pharmacy, 2014, 11 (3): 12 (in Chinese).

    [21] Li W J, Liu W L, Yang Y T et al. Study on dispensability of Chinese materia medica pharmaceutical system based on supramolecular imprinting template characteristics [J]. Chinese Traditional and Herbal Drugs, 2018, 49 (24): 5733–5739 (in Chinese).

    [22] Xia Z S, He F Y, Deng K W, et al. Special influence of molecular imprinting technology on traditional Chinese medicine theories [J]. China Journal of Chinese Materia Medica, 2013, 38 (8): 1266–1270 (in Chinese).

    [23] Liu H, Liu W L, Tang W H, et al. Exploring four properties of Chinese materia medica based on supramolecular “imprinting template” theory [J]. Chinese Traditional and Herbal Drugs, 2018, 49 (19): 4473–4479 (in Chinese).

    [24] He F Y, He H, Deng K W, et al. Exploration of research approaches of Chinese medicine’s pharmacology based on “imprinting templates” (medical element) of supramolecules [J]. China Journal of Chinese Materia Medica, 2015, 40 (21): 4313–4318 (in Chinese).

    [25] Zhou J, Liu H, Xiao M F, et al. Druggability and preparation modification of Chinese materia medica based on supramolecular “imprinted template” [J]. Chinese Traditional and Herbal Drugs, 2018, 49 (18): 4205–4211 (in Chinese).

    [26] Sambrook M R, Notman S. Supramolecular chemistry and chemical warfare agents: From fundamentals of recognition to catalysis and sensing [J]. Chem Soc Rev, 2014, 45 (5) : 9251-9267.

    [27] Ariga K, Kunitake T. Supramolecular Chemistry Fundamentals and Applications (Advanced Textbook) [M]. Heidelberg: Springer-Verlag, 2006.

    [28] Simantov R, Snyder S H. Morphine-like peptides in mammalian brain: Isolation, structure elucidation, and interactions with the opiate receptor [J]. Proc Natl Acad Sci USA, 1976, 73 (7) : 2515-2519.

    [29] Park S, Lee S Y, Park K M, et al. Supramolecular networking of macrocycles based on exo-coordination: From discrete to continuous frameworks [J]. Account Chem Res, 2012, 45 (3) : 391-403.

    [30] He F Y, Deng K W, Huang S et al. Establishment of the mathematic model of total quantum statistical moment standard similarity for application to medical theoretical research [J]. Acta Pharmaceutica Sinica, 2013, 48 (9): 1453–1458 (in Chinese).

    [31] He F Y, Deng K W, Liu W L et al. Experimental studies on pharmacokinetics of three components in Buyanghuanwu injection on base of total quantum statistical moment [J]. China Journal of Chinese Materia Medica, 2013, 38 (2): 253–262 (in Chinese).

    [32] Duan X P, He F Y, Zhou J et al. Study of additive properties of total chromatographic fingerprint for quantum statistical moment of Buyang Huanwu decoction [J]. China Journal of Chinese Materia Medica, 2011, 36 (23): 3247–3252 (in Chinese).

    [33] He F Y, Zhou H H, Deng K W, et al. A new qualitative and quantitative analytical method of chromatographic fingerprints: total quantum statistical moment [J]. Acta Pharmaceutica Sinica, 2008, 43 (2): 195–201 (in Chinese).

    [34] Hu C, He H, Yang Y T et al. HPLC fingerprint of Pien Tze Huang by total statistical moment and similarity method [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22 (8): 53–57 (in Chinese).

    [35] Yang Y T, Wu C Y, Wu D Z et al. Composition change in extraction process of Huanglian Jiedu Decoction by fingerprint division total statistical moment method [J]. Chinese Traditional and Herbal Drugs, 2014, 45 (15): 2157–2164 (in Chinese).

    [36] Zhou J, Deng K W, Duan X P et al. Study on calculation of fingerprint total quantum statistical moment (TQSM) and to decide integral condition [J]. Chinese Archives of Traditional Chinese Medicine, 2012, 30 (3): 505–508 (in Chinese).

    [37] Zhou J, Deng K W, He F Y et al. Qualitative and quantitative assessment on the HPLC fingerprint of BYHW Decoction extract by total quantum statistical moment [J]. Journal of Chinese Medicinal Materials, 2012, 35 (1): 133–137 (in Chinese).

    [38] Xie X G, He F Y, Shi J L et al. Studies on HPLC fingerprint of Safflower by total statistical moment method and principal component analysis [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18 (18): 64–69 (in Chinese).

This Article

ISSN:0253-2670

CN:12-1108/R

Vol 50, No. 04, Pages 829-835

February 2019

Downloads:5

Share
Article Outline

Abstract

  • 1 Principle of the experiment
  • 2 Test drugs and instruments
  • 3 Methods
  • 4 Results
  • 5 Analysis and discussion
  • References