Comparative study on contents and pharmacokinetics of raw and steamed Notoginseng Radix et Rhizoma and its anti-platelet aggregation activity

DI Ya-wei1 KANG An1 DI Liu-qing1 QIAN Jing1 LI Jun-song1 SHAN Jin-jun1 ZHAO Xiao-li1 BI Xiao-lin1

(1.Nanjing Engineering and Technological Research Center of TCM Pellets, Jiangsu Province (TCM) Key Laboratory of Children’s Respiratory Disease, Jiangsu Provincial TCM Engineering Technology, Research Center of High Efficient Drug Delivery System, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China 210046)

【Abstract】Objective To compare the relationship of ingredients and pharmacokinetics of steamed Notoginseng Radix et Rhizoma (the roots of Panax notoginseng) with different time (0, 2, 4, and 8 h), and accompany with its anti-platelet aggregation activity. Methods The components with different steaming duration were determined by HPLC method. Concentration of saponins in Notoginseng Radix et Rhizoma before and after steaming at different time points were detected by UPLC-MS/MS. Pharmacokinetic parameters of each compound were calculated using DAS 3.2.6 software. The anti-platelet aggregation activity was measured by platelet aggregation/clotting analyzer. Results The results showed that the steaming process reduced the contents of certain bioactive substances (NG-R1, Rg1, Rd, Rb1, and Re) and produced some new components (Rh1, Rg3, Rk3, and Rh4). Ginsenoside Rg3, deglycosylated metabolites of ginsenoside Rb1 possessed lower tmax than ginsenoside Rb1 that indicated the course of deglycosylation made faster absorption. Steamed Notoginseng Radix et Rhizoma had stronger antiplatelet activity, following higher antiplatelet and anticoagulant activities with increasing steaming durations. Conclusion The results inspire us that saponins may become more active ingredients after deglycosylation, saponins with deglycosylated in vitro become more and more active ingredients into the blood, which could make stronger anticoagulant activity.

【Keywords】 Notoginseng Radix et Rhizoma; steaming; ginsenoside; pharmacokinetics; platelet aggregation; deglycosylation;

【DOI】

【Funds】 Preponderant Discipline Construction Engineering Funded Project in Colleges and Universities in Jiangsu Province, China

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This Article

ISSN:0253-2670

CN: 12-1108/R

Vol 47, No. 01, Pages 95-100

January 2016

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Abstract

  • 1 Materials
  • 2 Methods and results
  • 3 Discussion
  • References