Network pharmacology-based study on mechanisms of Huanglian Jiedu Decoction impact on macrophage inflammation response

HENG Xia1,2 ZHU Bao-jie1,2 SUN Li-min1,2 PAN Lin-mei1,2 DUAN Jin-ao1,2 ZHANG Qi-chun1,2 ZHU Hua-xu1,2

(1.Jiangsu Collaboration Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, China 210023)
(2.Jiangsu Province Key Laboratory of High Technology Research, Nanjing University of Chinese Medicine, Nanjing, China 210023)

【Abstract】This study was designed to explore the impact of Huanglian Jiedu Decoction (HLJDT) on macrophage inflammation reaction using the network pharmacology method. Glycolysis, sphingolipid metabolism and glutamine metabolism were also investigated for “multi-component, multi-target and multi-pathway”, which supports a foundation for drug innovative research. The TCMSP database was used to screen the active components of HLJDT, the target protein predicted by Pharm Mapper database and the DAVID database for pathways annotation and analysis. The Cytoscape 3.2.1 software was used to construct the active component target–pathway network map and GENEMANIA database for protein interaction analysis. System Dock Database Site is used in verification of molecular docking. The results showed that 84 active ingredients were screened in HLJDT with a total of 111 target targets. Fourteen pathways are affected according to 13 macrophage-related inflammatory proteins, and 8 pathways including 34 target proteins from glycolysis, sphingolipid metabolism and glutamine metabolism. Inflammation-related proteins and metabolism-related proteins can interact with each other through physical correlation, protein co-expression, etc. Berberine, baicalin and geniposide combined well with 5 important targets. Huanglian Jiedu Decoction may act on the glycolysis and sphingolipid pathways to regulate macrophage inflammatory responses.

【Keywords】 network pharmacology; Huanglian Jiedu Decoction; inflammation; metabolism;

【DOI】

【Funds】 National Natural Science Foundation of China (81573635)

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This Article

ISSN:0513-4870

CN: 11-2163/R

Vol 53, No. 09, Pages 1449-1457

September 2018

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Article Outline

Abstract

  • Materials and methods
  • Results
  • Discussion
  • Conclusion
  • References