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多指标综合评价黄芪免疫活性的谱效相关性

洪妍1 刘小花1 陈亚丽1 王波1,2 陈宇1 师志强1 薛志远1 封士兰1

(1.兰州大学药学院, 甘肃兰州 730000)
(2.甘肃出入境检验检疫局综合技术中心, 甘肃兰州 730000)

【摘要】本文通过多指标研究测评黄芪小分子部位的指纹图谱与免疫活性的相关性, 揭示黄芪提高免疫功能的药效物质基础。采用10批黄芪药材小分子部位进行免疫抑制小鼠的免疫功能实验, 测定其吞噬指数、迟发型变态反应程度 (DTH) 、脏器指数以及干扰素-γ (IFN-γ) 、白细胞介素-4 (IL-4) 等药效值, 与HPLC-DAD/HPLC-ELSD指纹图谱的共有峰关联, 研究其谱效相关性。结果表明, 黄芪小分子部位中的多种成分对于增强免疫作用具有高度关联性。黄芪药材小分子部位提高小鼠免疫力是多种物质共同作用的结果。

【关键词】 黄芪;免疫活性;谱效关系;指纹图谱;

【DOI】

【基金资助】 甘肃省中药质量与标准研究重点实验室培育基地开放基金项目 (ZYZL16-007) ; 兰州市科技计划项目 (2016-3-111) ; 城关区科技计划项目 (2016-1-5) ;

Multi-index integrated evaluation of spectrum-effect relationship on immunocompetence of Radix Astragali

HONG Yan1 LIU Xiao-hua1 CHEN Ya-li1 WANG Bo1,2 CHEN Yu1 SHI Zhi-qiang1 XUE Zhi-yuan1 FENG Shi-lan1

(1.College of Pharmacy, Lanzhou University, Lanzhou , China 730000)
(2.Gansu Entry-Exit Inspection and Quarantine Bureau Central Laboratory of Technical Center, Lanzhou , China 730000)

【Abstract】The study was aimed to investigate the correlation between the immunocompetence and the fingerprints of supernatant extracts from Radix Astragali by multi-index integrated evaluation, and reveal the material basis of Radix Astragali improving the immunological function. After oral administration 10 batch of supernatant extracts from Radix Astragali on immunosuppressed mice, phagocytic index, delayed type hypersensitivity (DTH) degree, organs index and the level of cytokines IFN-γ, IL-4 (ELISAs) were measured, and each common peak from HPLC-DAD/HPLC-ELSD fingerprints were correlated with the above data. A number of components in supernatant extracts of Radix Astragali display a substantial correlation with the immunocompetence.Supernatant extracts of Radix Astragali can significantly enhance immunological function on mice, which is related to various components in Radix Astragali.

【Keywords】 Radix Astragali; immunocompetence; spectrum-effect relationship; fingerprint;

【DOI】

【Funds】 Open Funding Project of Gansu Provincial Key Laboratory Cultivation Base of Quality and Standard of Chinese Materia Medica (ZYZL16-007); Science and Technology Planning Project of Lanzhou City (2016-3-111); Science and Technology Planning Project of Chengguan District (2016-1-5);

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    References

    [1]Wang TX, Wang TX, Wu GC. Effect of Astragalus polysaccharide on immunological function in mice[J]. Shizhen Med Mater Med Res , 2009, 20: 1763–1764 (in Chinese).

    [2]Yan PY, Yu XH, Zhang DS, et al. TAF to the hypoimmunity mice on effect of T cell polarization[J]. J Zhejiang Coll Tradit Chin Med, 2008, 32: 163–164 (in Chinese).

    [3]Yang XM, Xu XW, Lu HL, et al. Study on activation of mouse peritoneal macrophage by Astragalus saponin[J]. Chin J Immunol, 2008, 24: 804–807 (in Chinese).

    [4]Xiao XH, Jin C, Yan D, et al. Proposition and practice on“integrative quality”in quality control for Chinese materia medica[J]. Chin Tradit Herb Drugs, 2010, 41: 505–508 (in Chinese).

    [5]Chinese Pharmacopoeia Committee. Pharmacopoeia of the People’s Republic of China [M]. Part 1. Beijing: China Medical Science Press, 2015: 302–303 (in Chinese).

    [6]Liang J, Liu XH, Ren Y, et al. Study on chromatography fingerprint of Radix Astragali with hyphenated technique of HPLC-DAD-ELSD[J]. Chin J Exp Tradit Med Formula, 2012, 18: 70–74 (in Chinese).

    [7]Su X, Wu LJ, Tu PF. RRLC-UV-MS fingerprint of Radix Astragali[J]. China J Chin Mater Med, 2010, 35: 732–735 (in Chinese).

    [8]Wang BL, Hu JP, Sheng L, et al. Chemical-pharmacokineticpharmacodynamic fingerprints of Schisandra chinensis alcoholic extract[J]. Acta Pharm Sin, 2013, 48: 734–740 (in Chinese).

    [9]Xu JJ, Lu JQ, Li XS, et al. Study on spectrum-effect relationship between fingerprints of essential oil from Shennong Xiangju and the in vitro antioxidant activities[J]. Chin J Pharm Anal, 2016, 36: 1823–1830 (in Chinese).

    [10]Cui F, Han ZH, Liu XH, et al. Spectrum-effect relationship of active fraction from Hedysari Radix on improving immunity[J]. Chin Tradit Herb Drugs, 2016, 47: 101–105 (in Chinese).

    [11]Liu XH, Liang J, Ren Y, et al. Spectrum-effect relationship in effect of improving immunity of Astragali Radix[J]. Chin Med Mat, 2012, 35: 1978–1981 (in Chinese).

    [12]Liu XH. Study on Spectrum-effect Relationship and Metabolism of Anti-gastric Ulcer Effects in Radix Astragali [D]. Lanzhou: Lanzhou University, 2015 (in Chinese).

    [13]Liu SF, Dang YG, Fang ZG. Gray System Theory and Its Application [M]. Beijing: Science Press, 2004 (in Chinese).

This Article

ISSN:0513-4870

CN: 11-2163/R

Vol 52, No. 05, Pages 785-789

May 2017

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Abstract

  • Materials and Methods
  • Results
  • Discussion
  • References