Metabonomic study of metformin in type Ⅱ diabetic rats
(2.Guangdong Provincial Key Lab of Research and Development in Traditional Chinese Medicine, Guangzhou, China 510095)
(3.Fifth Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou, China 510405)
【Abstract】 Aim To study the mechanism of metformin in the treatment of type 2 diabetic mellitus (T2DM) rats. Methods Rats were randomly divided into normal group, model group and metformin group with 10 rats in each group. Four weeks after induction with HFSD, 35 mg·kg−1 STZ was injected intramuscularly, and the experiment was completed after eight weeks of administration. UPLC/ESI-TOF-MS was used to study the effects of metformin on metabolites in serum of T2DM rats, and qPCR was used to find its target. Results Compared with normal group, the body weight, pancreatic index and insulin level in model group decreased significantly (P < 0.05), the liver index, GLU, TC, TG and FFA levels increased significantly (P < 0.01), OGTT was significantly impaired, while metformin could significantly increase the body weight, pancreatic index and insulin level (P < 0.05), reduce the liver index, GLU, TC, TG and FFA levels (P < 0.05), and improve OGTT. After metabonomics, 17 biomarkers were obtained. After verification, metformin was found to regulate the synthesis and metabolism of cholesterol, fatty acids, bile acids and phospholipids. Conclusions Metformin can improve the lipid metabolism disorder of T2 DM by regulating the synthesis and metabolism of cholesterol, fatty acids, bile acids and phospholipids.
【Keywords】 metabolic profile; biomarker; type 2 diabetic mellitus; metformin; bile acids; fatty acid;
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