Cardioprotective effect of recombinant adeno-associated virus mediated nerve growth factor gene transfection in diabetic rats

YI Jian-min1 YUE Wei2 GAO Pan1 ZHANG Wei-nan1

(1.Dept of Anesthesiology, Shanxi Medical University, Taiyuan, China 030001)
(2.Dept of Anesthesiology, the Second Hospital of Shanxi Medical University, Taiyuan, China 030001)
【Knowledge Link】nerve growth factor; transfection

【Abstract】 Aim To investigate the feasibility of recombinant adeno-associated virus serotype9 (rAAV9) mediated nerve growth factor (NGF) transfection in diabetic rats by intramyocardial injection, and to confirm the protective effect of this method on diabetic cardiac autonomic neuropathy. Methods At the 4th week after the establishment of diabetic model, the recombinant adeno-associated virus (rAAV9-NGF) carrying NGF gene was injected into the heart of rats. Five weeks later, the distributions of NGF, calcitonin gene-related peptide (calcitonin gene-related peptide, CGRP) and nerve fibers were detected by immunofluorescence. Hemodynamic parameters were used to evaluate cardiac function, and ELISA method was used to detect the expression of NGF, CGRP in myocardium. Results The exogenous gene NGF mediated by rAAV9 could be stably transfected and over-expressed in the myocardium of diabetic rats. The left ventricular systolic pressure, heart rate, and maximal rate of increase of left ventricular pressure significantly decreased, the left ventricular end-diastolic pressure and the maximal decrease rate of left ventricular pressure significantly increased, and the cardiac function was improved. The NGF, CGRP proteins in myocardial tissues were up-regulated, and the immunofluorescence showed that the decrease of NGF, CGRP and nerve fibers in myocardial tissues could be partially reversed after transfection. Conclusions Intramyocardial injection of NGF gene can effectively enhance the expression of NGF and promote the growth of nerve fiber, exerting a protective effect in diabetic rat heart.

【Keywords】 nerve growth factor; calcitonin gene-related peptide; transfection; recombinant adeno-associated virus; diabetes peripheral neuropathy; heart disease;


【Funds】 National Natural Science Foundation of China (34100940) Special Research Fund of Doctoral Programs of Higher Education, Ministry of Education (20131417120001) Higher Education Science and Technology Innovation Project of Shanxi Provincial Education Department (2013120)

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This Article


CN: 34-1086/R

Vol 35, No. 03, Pages 424-429

March 2019


Article Outline



  • 1 Materials and methods
  • 2 Results
  • 3 Discussion
  • References