The therapeutic effect of rh-aFGF carbomer 940 gel on wound healing in type Ⅰ diabetic SD rat models

YANG Xuan-xin1 HUI Qi1 CAO Gao-zhong2 LIU Jian-guo3 DU Xiao-xiao1 LI Xiao-kun1,4 WANG Xiao-jie1

(1.College of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang, China 325035)
(2.First People’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China 325000)
(3.School of Pharmacy, Second Military Medical University, Shanghai, China 200433)
(4.Institute of Biological Sciences, Wenzhou University, Wenzhou, Zhejiang, China 325035)

【Abstract】Aim To study the therapeutic effect of recombinant human acidic fibroblast growth factor (rh-aFGF) carbomer 940 gel in the treatment of skin wound healing in type I diabetic rats. Methods Two types of skin trauma models, namely, full-thickness wound and scalded wound, were established in a model of type I diabetes mellitus using STZ-induced SD rats. The rats were divided into control group, vehicle group, 90 AU rh-aFGF gel group and 270 AU rh-aFGF gel group in each skin wound models. The wound area and wound healing rate were used to evaluate the therapeutic effect. The growth of fibroblasts, fibrocytes, collagen fibers and vessel capillaries in the wound was observed using HE staining and analyzed by semiquantitative score. Results The rh-aFGF carbomer gel significantly reduced the traumatic area as well as promoted the wound healing rate of the skin trauma model of SD rats of type I diabetes mellitus (P < 0.05). HE staining showed that rh-aFGF carbomer gel significantly promoted the pathological score of fibroblasts and collagen fibers (P < 0.05). Conclusions rh-aFGF carbomer gel might play a protective role in micro-environment of wound and rh-aFGF, which could benefit for proliferation of fibroblasts and collagen, therefore promoting the healing process of skin wound in SD rats with type I diabetes mellitus, and it might be expected to be a new preparation for the treatment of chronic trauma in diabetes mellitus.

【Keywords】 recombinant acid fibroblast growth factor; skin trauma; Carbomer 940; gel preparation; skin wound; healing rate;


【Funds】 National Natural Science Foundation of China (81601695) Wenzhou Public Welfare Social Development (Medical and Health) Technology Support Project (Y20150104)

Download this article


    [1] Chammas N K, Hill R L, Edmonds M E. Increased mortality in diabetic foot ulcer patients: the significance of ulcer type [J]. J Diabetes Res, 2016, 2016: 2879809.

    [2] Danmusa U M, Terhile I, Nasir I A, et al. Prevalence and healthcare costs associated with the management of diabetic foot ulcer in patients attending Ahmadu Bello University Teaching Hospital, Nigeria [J]. Int J Health Sci (Qassim), 2016, 10 (2): 219–28.

    [3] Kobielak A, Zakrzewska M, Kostas M, et al. Protease resistant variants of FGF1 with prolonged biological activity [J]. Protein Pept Lett, 2014, 21 (5): 434–43

    [4] Perry R J, Lee S, Ma L, et al. FGF1 and FGF19 reverse diabetes by suppression of the hypothalamic–pituitary–adrenal axis [J]. Nat Commun, 2015, 286 (6): 6980.

    [5] Suh J M, Jonker J W, Ahmadian M, et al. Endocrinization of FGF1 produces a neomorphic and potent insulin sensitizer [J]. Nature, 2014, 513 (7518): 436–9.

    [6] Sun K, Scherer P E. The PPARγ–FGF1 axis: an unexpected mediator of adipose tissue homeostasis [J]. Cell Res, 2012, 22 (10): 1416–8.

    [7] Sun R P, Zhao L K, Sun J, et al. Observation on the effect of external use lyophilized recombinant human acidic fibroblast growth factor in the treatment of shallow 2 degree burn [J]. Chin J Rural Med Pharm, 2015, 22 (9): 11–2 (in Chinese).

    [8] Chen X, Ward S C, Cederbaum A I, et al. Alcoholic fatty liver is enhanced in CYP2A5 knockout mice: The role of the PPARα–FGF21 axis [J]. Toxicology, 2017, 379: 12–21.

    [9] Bon Durant L D, Ameka M, Naber M C, et al. FGF21 regulates metabolism through adipose–dependent and–independent mechanisms [J]. Cell Metab, 2017, 25 (4): 935–44.

    [10] Guo Y Y, Chang L P, Zhang J F, et al. Effect of Tongxinluo on micro-environment of peripheral blood derived mesenchymal stem cells transplantation in diabetic foot rat [J]. Chin Pharmacol Bull, 2017, 33 (7): 1032–3 (in Chinese).

    [11] Blakytny R, Jude E. The molecular biology of chronic wounds and delayed healing in diabetes [J]. Diabet Med, 2006, 23 (6): 594–608.

    [12] Wu J, Zhu J, He C, et al. Comparative study of heparin–poloxamer hydrogel modified b FGF and aFGF for in vivo wound healing efficiency [J]. ACS Appl Mater Interfaces, 2016, 8 (29): 18710–21.

    [13] Lund R G, Silva A F, Piva E, et al. Clinical evaluation of two desensitizing treatments in southern Brazil: a 3-month follow-up [J]. Acta Odontol Scand, 2013, 71 (6): 1469–74.

    [14] Xu M, Sun M, Qiao H, et al. Preparation and evaluation of colon adhesive pellets of 5-aminosalicylic acid [J]. Int J Pharm, 2014, 468 (1–2): 165–71.

    [15] Zhao Y Y, Huang X Y, Chen Z W. Daintain/AIF-1 (allograft inflammatory factor-1) accelerates type Ⅰ diabetes in NOD mice [J]. Biochem Biophys Res Commun, 2012, 427 (3): 513–7.

    [16] Jiang J Y, Xu H, Bai X T, et al. Research in skin healing and repair function and mechanism of Hibiscus rosa-sinensis Linn bud extract [J]. Chin Pharmacol Bull, 2015, 31 (8): 1085–91 (in Chinese).

This Article


CN: 34-1086/R

Vol 34, No. 04, Pages 557-562

April 2018


Article Outline


  • 1 Materials and methods
  • 2 Results
  • 3 Discussion
  • References