Repair effect of meisoindigo on myocardial damage in type 1 diabetes mellitus in rats
(2.Institute of Materia Medica, Shandong Academy of Medical Sciences 250062)
(3.Key Laboratory for Biotech-Drugs Ministry of Health 250062)
(4.Key Laboratory for Rare and Uncommon Diseases of Shandong Province, Jinan, China 250062)
【Abstract】Aim To observe the influence of meisoindigo on the alteration of Wnt/β-catenin signaling in type 1 diabetic rats’ myocardium and clarify its role in the development of diabetic cardiomyopathy. Methods The type 1 diabetes rat model was established by injection of streptozocin after one-week adaptive feeding. The successful modeling rats were randomly divided into DM model group of 4 weeks and 8 weeks, meisoindigo group of 4 weeks and 8 weeks. Fasting blood glucose (FBG) levels were tested. HE staining was used to observe the pathological changes of myocardial structures. The alteration of GSK-3β, p-GSK-3β, Wnt2, β-catenin, NF-κB-p65, and p-NF-κB-p65 in myocardium was determined by Western blot and immunohistochemistry. Results Compared with control group, FBG levels of type 1 diabetic rats significantly increased (P < 0.01), while body weight levels significantly decreased (P < 0.01); compared with DM group, FBG levels of 8 weeks meisoindigo group significantly decreased (P < 0.01). Myocardial histological analysis revealed that DM induced myocardial focal myocyte hypertrophy, solubility, necrosis, fiber tissue hyperplasia; compared with DM group, these symptoms were eased in meisoindigo group of 4 weeks and 8 weeks. Compared with control group, the expression of p-GSK-3β, Wnt2, β-catenin, p-NF-κB-p65 level increased, especially with DM group of 8 weeks (P < 0.01). The expression of p-GSK-3β, Wnt2, β-catenin, p-NF-κB-p65 level in meisoindigo group of 4 weeks and 8 weeks decreased significantly (P < 0.01). Conclusions The repair effect of meisoindigo on myocardial damage in type 1 diabetic rats may be caused by lowering the expression of proteins in Wnt/β-catenin signaling and inhibiting the activation of Wnt/β-catenin signaling pathway, participating in the repair of myocardial damage and inflammatory in diabetic rats. Further researches on its mechanism in repairing diabetic myocardial damage may find new therapeutic targets for diabetic cardiomyopathy.
【Keywords】 type 1 diabetes; diabetic cardiomyopathy; meisoindigo; GSK-3β; Wnt2; β-catenin;
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