培菌白蚁菌圃和粪便微生物多样性分析

张硕1 林子佳1 肖元玺1 李净净1 李枝1 倪金凤1

(1.山东大学微生物技术研究院微生物技术国家重点实验室, 山东青岛 266237)

【摘要】【背景】培菌白蚁是属于白蚁科的一类与鸡枞菌属真菌共生的高等白蚁,其与体内肠道微生物和体外菌圃微生物形成三维共生体系。【目的】分析培菌白蚁菌圃和粪便的微生物多样性,并与肠道微生物进行比较。【方法】通过Illumina MiSeq高通量测序方法对培菌白蚁菌圃和粪便样品进行细菌16S rRNA基因和真菌ITS测序分析。【结果】高通量测序获得培菌白蚁菌圃和粪便样品细菌和真菌的有效序列和OTU数目。5个样品细菌OTU数目在90-199之间,而真菌OTU在10-58之间,细菌的种类多样性明显大于真菌。不论是细菌还是真菌,粪便样品的OTU数目多于菌圃样品。经物种分类分析,菌圃样品主要优势细菌是变形菌门(Proteobacteria),其相对含量超过82.4%;其次是拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes);粪便样品中优势细菌为拟杆菌门,其次是变形菌门,粪便优势菌属为别样杆菌属和营发酵单胞菌属,这与培菌白蚁肠道菌多样性组成一致。培菌白蚁菌圃和粪便样品共生真菌主要为担子菌门(Basidiomycota)和子囊菌门(Ascomycota)。菌圃优势真菌为鸡枞菌属(Termitomyces),相对含量在51.83%以上,菌圃中还鉴定到炭角菌属(1%,Xylaria)。【结论】为今后培菌白蚁-体内外微生物共生关系研究以及微生物的分离培养提供了依据和参考。

【关键词】 培菌白蚁; 菌圃; 粪便; 细菌和真菌多样性;

【DOI】

【基金资助】 国家自然科学基金(31970119,31272370) National Natural Science Foundation of China (31970119;31272370)

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This Article

ISSN:0253-2654

CN: 11-1996/Q

Vol 47, No. 10, Pages 3091-3101

October 2020

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摘要

  • 1 材料与方法
  • 2 结果与分析
  • 3 讨论与结论
  • 参考文献