Exploring mechanisms for Bacillus amyloliquefaciens-mediated production performance improvement of laying hens based on cecal microflora and ileum mucosal gene expression

ZHANG Hong-Na1 ZHOU Yu-Fa2 CUI Na1 PANG Quan-Hai2 CHANG Wei-Shan3

(1.College of Biological Science and Engineering, Hebei University of Economics and Business, Shijiazhuang, Hebei, China 050061)
(2.College of Animal Science and Technology, Shanxi Agricultural University, Jinzhong, Shanxi, China 030801)
(3.College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong, China 271000)
【Knowledge Link】exsanguination

【Abstract】[Background] Bacillus amyloliquefaciens BLCC1-0238 supplementation has been reported to effectively improve production performance and egg quality of laying hens in our previous study, the underlying mechanism, however, remains poorly understood. [Objective] The current study was conducted to examine the potential mechanism of B. amyloliquefaciens BLCC1-0238 to increase the production performance of laying hens by evaluating cecal microflora and gene expression in ileum mucosa. [Methods] High-throughput sequencing technology was used to compare the differences of cecal microflora composition and gene transcription level of ileum mucosa between the basal diet group (group C) and B. amyloliquefaciens BLCC1-0238 supplementation group (0.06%, 2.0 × 1010 CFU/g, group T). [Results] B. amyloliquefaciens BLCC1-0238 supplementation can produce higher indexes of Chao1 and Shannon, which demonstrated increased diversity of cecal microflora. At the phylum level, the Firmicutes/Bacteroidetes ratio significantly increased, while relative abundance of Fusobacteria and Proteobacteria reduced. At the genus level, the relative abundance of Phascolarctobacterium, Lactobacillus, Ruminococcaceae UCG-014, Anaerotruncus, Ruminiclostridium 9, and Christensenellaceae_R-7_ group all elevated. These results indicated that B. amyloliquefaciens BLCC1-0238 supplementation altered the cecal microflora composition from different levels. With respect to gene expression change in ileum mucosa, 356 differentially expressed genes were identified, among which 199 genes were up-regulated and 157 genes were down-regulated. Bioinformatics analysis found that these up-regulated genes were involved in the signaling pathways associated with nutrition metabolism, such as glycine, serine and threonine metabolism, starch and sucrose metabolism, and galactose metabolism, which may accelerate nutrition absorption of laying hens. [Conclusion] B. amyloliquefaciens BLCC1-0238 supplementation can effectively improve laying hen production performance via increasing the diversity of cecal microflora and promoting nutrition absorption of laying hens, providing solid evidence for its practical application in the future.

【Keywords】 Laying hens; Bacillus amyloliquefaciens BLCC1-0238; Genomics; Transcriptomics; Production performance;

【DOI】

【Funds】 National Natural Science Foundation of China (81903282) Key Research and Development Project of Shandong Province (2015GSF19023)

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This Article

ISSN:0253-2654

CN: 11-1996/Q

Vol 47, No. 08, Pages 2516-2525

August 2020

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Abstract

  • 1 Materials and methods
  • 2 Results
  • 3 Discussion and conclusions
  • References