Isolation, identification and characterization of an antagonistic bacterium against Bipolaris papendorfii

ZHANG Xin-Jie1 LIU Yan-Ce1 QIAN Xin-Yu1 XIAO Sheng-Lin1 YU Bo1 DONG Jin-Gao2 HAO Zhi-Min1

(1.College of Life Sciences, Hebei Agricultural University, Baoding, Hebei, China 071001)
(2.College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, China 071001)

【Abstract】 [Background] Bipolaris papendorfii is the main fungal pathogen of Curvularia leaf spot, and harmful to agricultural production. At present, the use of bacteria to control B. papendorfii becomes a research focus in this field. [Objective] To screen the strains with high antagonistic activity against B. papendorfii, and identify the antagonistic mechanism. [Methods] Twenty-two bacterial strains were isolated from the soil under the surface of maize field by the method of plate confrontation. One strain L-14 with high antagonistic activity to B. papendorfii was obtained by repeated screening. [Results] Strain L-14 with the highest antagonism was obtained by using the plate diffusion method. After morphological observation, 16S rRNA gene sequence analysis, physiological and biochemical characteristics detection, the strain was finally identified as Bacillus subtilis. Moreover, the strain is a wide-spectrum antagonistic biocontrol strain with inhibitory effects on Fusarium proliferatum, Fusarium graminearum, Exserohilum turcicum, Bipolaris papendorfii and Botrytis cinerea. Then, we studied the antibacterial mechanism of strain L-14 and obtained that the fermentation crude protein extracts had no effect on the morphology of the aerial hyphae of B. papendorfii but can cause substrate mycelial aberration, and had an inhibitory effect on spore germination. [Conclusion] The antagonistic bacterium B. subtilis obtained in this study has a broad spectrum and high antagonistic activity in the control of plant diseases, and can effectively control Curvularia leaf spot.

【Keywords】 Bipolaris papendorfii; Isolation; Screening; Identification; Bacillus subtilis; Antagonism;

【DOI】

【Funds】 National Key Research and Development Program of China (2018YFD0200607-02) China Agriculture Research System (CARS-02)

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This Article

ISSN:0253-2654

CN: 11-1996/Q

Vol 47, No. 08, Pages 2417-2424

August 2020

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Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion and conclusion
  • References