Transcriptional regulation of small RNA EsrE in Escherichia coli

SONG Jie1 HOU Bing-Bing1 YE Jiang1 WU Hai-Zhen1 ZHANG Hui-Zhan1

(1.State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, China 200237)

【Abstract】[Background] Small RNA EsrE affects cell growth by regulating the expression of succinate dehydrogenase in Escherichia coli, and the exploration of its regulatory mechanism is conducive to the understanding of EsrE effect on cell growth. [Objective] To explore the transcriptional regulation mechanism of small RNA EsrE in E. coli. [Methods] Transcriptional regulators were screened out using a dual-plasmid reporter system; the interaction between RpoH and PesrE was verified by electrophoretic mobility shift assay (EMSA); qRT-PCR shows effects of the transcriptional regulators on EsrE. [Results] The results of reporter system demonstrated that RpoH up-regulated PesrE and FabZ down-regulated PesrE. The results of EMSAs showed that RpoH bound the PesrE fragment directly, while FabZ did not. [Conclusion] RpoH is involved in the regulation of esrE transcription by directly binding with the PesrE promoter, while FabZ is involved in the transcriptional regulation of EsrE indirectly by other ways.

【Keywords】 Escherichia coli; sRNA EsrE; Transcriptional regulator; RpoH; RNA polymerase;

【DOI】

【Funds】 National Natural Science Foundation of China (31070073, 31372550)

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(Translated by LIU WJ)

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This Article

ISSN:0253-2654

CN: 11-1996/Q

Vol 47, No. 05, Pages 1430-1440

May 2020

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results
  • 3 Discussion and conclusion
  • References