Isolation, identification and mutation breeding of Chlorella sorokiniana TX with high protein content

XIE Feng-Xing1 ZHOU Ke1 ZHANG Feng-Feng1 ZHAO Qiong1 SUN Hai-Bo1 WANG Shu1 ZHAO Yu-Jie1

(1.Tianjin Research Center of Agricultural Biotechnology, Tianjin, China 300384)

【Abstract】[Background] Chlorella sp. contains 20 amino acids needed for animal growth and is rich in proteins, polysaccharides, unsaturated fatty acids, carotenoids, astaxanthin and vitamins. It can be used as high-quality natural bait for fish, shrimp and shellfish. [Objective] Chlorella TX was isolated from aquaculture environment. The method of mutagenesis was used to breed mutants with high biomass and protein content to obtain excellent algae resources for natural bait production in aquaculture. [Methods] Chlorella TX, a fast-growing and protein-rich alga, was screened out from the aquaculture environment as the starting strain. The 18S rRNA gene sequence analysis was employed to identify strain Chlorella TX, and then ultraviolet mutagenesis, ethyl methane sulfonate (EMS) mutagenesis, compound mutagenesis, 96-well plate high-throughput screening and progressive repeated screening were used to breed the mutants with high biomass and protein content. [Results] TX was identified as Chlorella sorokiniana by 18S rRNA gene sequence analysis. Eight mutants with genetic stability and growing fast were screened out from 540 mutants after progressive repeated screening. The total protein, soluble protein content and dry weight of mutant H10 were 64.2%, 0.44 g/L and 0.72 g/L respectively, which were 3.4%, 15.8% and 26.2% higher than those of strain TX. [Conclusion] The mutant H10 with high protein and growing fast can be used for natural bait production.

【Keywords】 Chlorella sorokiniana; Mutation breeding; Total protein; Soluble protein;

【DOI】

【Funds】 Innovative Research and Experimental Projects for Young Researchers of Tianjin Academy of Agricultural Sciences (2018007) Science and Technology Demonstration Project for Integrated Development of Primary, Secondary and Tertiary Industries (17ZXYENC00070) Tianjin Enterprise Technology Commissioner Project (19JCTPJC57700)

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This Article

ISSN:0253-2654

CN: 11-1996/Q

Vol 47, No. 03, Pages 821-828

March 2020

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results
  • 3 Discussion and conclusions
  • References