Isolation, identification and optimization of degradation conditions of a deltamethrin-degrading strain

HU Qiong1 TANG Jie1 LEI Dan1 WU Min1 SUN Qing1 ZHANG Qing2

(1.School of Food and Bioengineering, Xihua University, Chengdu, Sichuan, China 610039)
(2.Institute of Ancient Brewing, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, China 610039)

【Abstract】[Background] The degradation of pyrethroid pesticides is important for food safety and environmental health, and biodegradation is considered to be a green effective solution. [Objective] The strain with high ability to degrade deltamethrin (DM) was isolated from the strawberry rhizosphere soil contaminated by pyrethroid pesticides for a long term, and the degradation rate of the DM-degrading strain was improved by optimizing the medium and degradation conditions. [Methods] The DM-degrading strain was screened by enrichment domestication, isolation and purification and identified based on morphological and physio-biochemical properties as well as 16S rRNA sequence analysis. The degradation conditions were optimized by Plackett-Burman design, steepest ascent path design and Box-Behnken design. [Results] Strain LH-1-1 was identified as Acinetobacter junii, and it could degrade 53.43% DM (100 mg/L) within 96 h at initial conditions. The optimized conditions were 75 mg/L DM, 3 g/L tryptone, pH 6.8, 1.5 g/L (NH4)2SO4, 0.01 g/L FeCl3, inoculation amount of 5%, strain age of 12 h and culture temperature at 30 °C. The degradation rate of DM under these conditions reached 82.36% within 96 h, which was 28.93% higher than that at initial conditions. [Conclusion] A. junii LH-1-1, a highly efficient DM-degrading strain, can be used as excellent microbial resource for the bioremediation of environment polluted by DM or pyrethroids.

【Keywords】 Deltamethrin; Plackett-Burman design; Box-Behnken design; Acinetobacter junii LH-1-1;


【Funds】 Application Foundation Project of Sichuan Provincial Department of Science and Technology (2019YJ0389) Key Scientific Research Fund of Xihua University (z1310525) Graduate Student Innovation Fund of Xihua University (ycjj2019122)

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(Translated by WANG YX)


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This Article


CN: 11-1996/Q

Vol 47, No. 03, Pages 699-709

March 2020


Article Outline


  • 1 Materials and methods
  • 2 Results
  • 3 Discussion and conclusion
  • References