Isolation, identification and optimization of degradation conditions of a deltamethrin-degrading strain

HU Qiong1 TANG Jie1 LEI Dan1 WU Min1 SUN Qing1 ZHANG Qing2

(1.School of Food and Bioengineering, Xihua University, Chengdu, Sichuan, China 610039)
(2.Institute of Ancient Brewing, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, China 610039)

【Abstract】[Background] The degradation of pyrethroid pesticides is important for food safety and environmental health, and biodegradation is considered to be a green effective solution. [Objective] The strain with high ability to degrade deltamethrin (DM) was isolated from the strawberry rhizosphere soil contaminated by pyrethroid pesticides for a long term, and the degradation rate of the DM-degrading strain was improved by optimizing the medium and degradation conditions. [Methods] The DM-degrading strain was screened by enrichment domestication, isolation and purification and identified based on morphological and physio-biochemical properties as well as 16S rRNA sequence analysis. The degradation conditions were optimized by Plackett-Burman design, steepest ascent path design and Box-Behnken design. [Results] Strain LH-1-1 was identified as Acinetobacter junii, and it could degrade 53.43% DM (100 mg/L) within 96 h at initial conditions. The optimized conditions were 75 mg/L DM, 3 g/L tryptone, pH 6.8, 1.5 g/L (NH4)2SO4, 0.01 g/L FeCl3, inoculation amount of 5%, strain age of 12 h and culture temperature at 30 °C. The degradation rate of DM under these conditions reached 82.36% within 96 h, which was 28.93% higher than that at initial conditions. [Conclusion] A. junii LH-1-1, a highly efficient DM-degrading strain, can be used as excellent microbial resource for the bioremediation of environment polluted by DM or pyrethroids.

【Keywords】 Deltamethrin; Plackett-Burman design; Box-Behnken design; Acinetobacter junii LH-1-1;

【DOI】

【Funds】 Application Foundation Project of Sichuan Provincial Department of Science and Technology (2019YJ0389) Key Scientific Research Fund of Xihua University (z1310525) Graduate Student Innovation Fund of Xihua University (ycjj2019122)

Download this article

(Translated by WANG YX)

    References

    [1] Cycoń M, Piotrowska-Seget Z. Pyrethroid-degrading microorganisms and their potential for the bioremediation of contaminated soils: a review [J]. Frontiers in Microbiology, 2016, 7: 1463

    [2] Chung SWC, Lam CH. Development and validation of a method for determination of residues of 15 pyrethroids and two metabolites of dithiocarbamates in foods by ultra-performance liquid chromatography-tandem mass spectrometry [J]. Analytical and Bioanalytical Chemistry, 2012, 403 (3): 885–896

    [3] Liu B, Tang J, Chen TT, et al. Screening and characterization of a 3-phenoxybenzoic acid-degrading Enterobacter ludwigii [J]. Acta Microbiologica Sinica, 2018, 58 (5): 830–841 (in Chinese)

    [4] Hao XX, Zhang XQ, Duan BH, et al. Screening and genome sequencing of deltamethrin-degrading bacterium ZJ6 [J]. Current Microbiology, 2018, 75 (11): 1468–1476

    [5] Chen SH, Lai KP, Li YN, et al. Biodegradation of deltamethrin and its hydrolysis product 3-phenoxybenzaldehyde by a newly isolated Streptomyces aureus strain HP-S-01 [J]. Applied Microbiology and Biotechnology, 2011, 90 (4): 1471–1483

    [6] Zhang H, Zhang YM, Hou ZG, et al. Biodegradation potential of deltamethrin by the Bacillus cereus strain Y1 in both culture and contaminated soil [J]. International Biodeterioration & Biodegradation, 2016, 106: 53–59

    [7] Hou SF. Preparation and application of deltamethrin-degrading bacteria agent [D]. Guiyang: Master’s Thesis of Guizhou University, 2016 (in Chinese)

    [8] Yang XT. Study on isolation and degrading characteristics of deltamethrin-degrading bacteria [D]. Changchun: Master’s Thesis of Jilin Agricultural University, 2008 (in Chinese)

    [9] Su ZJ. Isolation, identification and degradation characteristics of deltamethrin-degrading bacterial strain [D]. Shanghai: Master’s Thesis of Shanghai Ocean University, 2018 (in Chinese)

    [10] Xu ZZ, Wang MY, Du JP, et al. Isolation, identification and fermentation optimization of an antagonistic bacterial strain HQB-1 against banana wilt disease [J]. Microbiology China, 2019, 46 (7): 1611–1618 (in Chinese)

    [11] Lu GY, Zhang ZF, Pan HJ, et al. Optimization of submerged cultivation medium components for lentinan production by Lentinula edodes using response surface methodology [J]. Mycosystema, 2010, 29 (1): 106–112 (in Chinese)

    [12] Liu B, Tang J, Chen TT, et al. Isolation and identification of two pyrethroid pesticides degradation strains and research of degradative capabilities [J]. Science and Technology of Food Industry, 2017, 38 (4): 214–219, 224 (in Chinese)

    [13] Tang J, Liu B, Shi Y, et al. Isolation, identification, and fenvalerate-degrading potential of Bacillus licheniformis CY-012 [J]. Biotechnology & Biotechnological Equipment, 2018, 32 (3): 574–582

    [14] Sánchez-González M, Álvarez-Uribe H, Rivera-Solís R, et al. Analysis of a phenol-adapted microbial community: degradation capacity, taxonomy and metabolic description [J]. Journal of Applied Microbiology, 2019, 126 (3): 771–779

    [15] Liu J, Cai YG, Wang XW, et al. Preparation and rheological properties of nutritional seabuckthorn meal replacement powder [J]. Science and Technology of Food Industry, 2019, 40 (8): 163–169 (in Chinese)

    [16] Weston DP, Holmes RW, Lydy MJ. Residential runoff as a source of pyrethroid pesticides to urban creeks [J]. Environmental Pollution, 2009, 157 (1): 287–294

    [17] Perry MJ, Venners SA, Barr DB, et al. Environmental pyrethroid and organophosphorus insecticide exposures and sperm concentration [J]. Reproductive Toxicology, 2007, 23 (1): 113–118

    [18] Guo XQ, Wang XJ, Sun AL, et al. Advances of studies on microbial degradation of pyrethroid insecticides [J]. China Biotechnology, 2017, 37 (5): 126–132 (in Chinese)

    [19] Zhang QZ, Wang DC, Li MM, et al. Isolation and characterization of diesel degrading bacteria, Sphingomonas sp. and Acinetobacter junii from petroleum contaminated soil [J]. Frontiers of Earth Science, 2014, 8 (1): 58–63

    [20] Ohadi M, Dehghannoudeh G, Shakibaie M, et al. Isolation, characterization, and optimization of biosurfactant production by an oil-degrading Acinetobacter junii B6 isolated from an Iranian oil excavation site [J]. Biocatalysis and Agricultural Biotechnology, 2017, 12: 1–9

    [21] Anwar F, Hussain S, Ramzan S, et al. Characterization of reactive red-120 decolorizing bacterial strain Acinetobacter junii FA10 capable of simultaneous removal of azo dyes and hexavalent chromium [J]. Water, Air, & Soil Pollution, 2014, 225 (8): 2017

    [22] Han YH, Fu T, Wang SS, et al. Efficient phosphate accumulation in the newly isolated Acinetobacter junii strain LH4 [J]. 3 Biotech, 2018, 8 (7): 313

    [23] Yang L, Ren YX, Liang X, et al. Nitrogen removal characteristics of a heterotrophic nitrifier Acinetobacter junii YB and its potential application for the treatment of high-strength nitrogenous [J]. Bioresource Technology, 2015, 193: 227–233

    [24] Shi Y, Tang J, Yao K, et al. Isolation and characterization of fenvalerate-degrading strain and optimization of degradation conditions [J]. Science and Technology of Food Industry, 2016, 37 (2): 217–222, 243 (in Chinese)

    [25] Chen SH, Deng YY, Chang CQ, et al. Pathway and kinetics of cyhalothrin biodegradation by Bacillus thuringiensis strain ZS-19 [J]. Scientific Reports, 2015, 5: 8784

    [26] Xiao Y, Chen SH, Gao YQ, et al. Isolation of a novel beta-cypermethrin-degrading strain Bacillus subtilis BSF01 and its biodegradation pathway [J]. Applied Microbiology and Biotechnology, 2015, 99 (6): 2849–2859

    [27] Zhao HY, Geng YC, Chen L, et al. Biodegradation of cypermethrin by a novel Catellibacterium sp. strain CC-5 isolated from contaminated soil [J]. Canadian Journal of Microbiology, 2013, 59 (5): 311–317

    [28] Huang WY, Hou SF, Long YH, et al. Isolation, screening and application potential evaluation of deltamethrin-degrading microorganism [J]. Chinese Journal of Pesticide Science, 2018, 20 (5): 643–651 (in Chinese)

This Article

ISSN:0253-2654

CN: 11-1996/Q

Vol 47, No. 03, Pages 699-709

March 2020

Downloads:0

Share
Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results
  • 3 Discussion and conclusion
  • References