Carbon metabolism gene gabT regulates γ-aminobutyric acid metabolism of Streptomyces venezuelae Snea253 on nematicidal activity

YU Dong-Mei1 ZHU Feng2 XING Zhi-Fu1 FAN Hai-Yan1 ZHU Xiao-Feng1 DUAN Yu-Xi1 WANG Yuan-Yuan3 LIU Xiao-Yu4 CHEN Li-Jie1

(1.College of Plant Protection, Shenyang Agricultural University, Shenyang, Liaoning, China 110866)
(2.Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling, Jilin, China 130033)
(3.College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, China 110866)
(4.College of Science, Shenyang Agricultural University, Shenyang, Liaoning, China 110866)

【Abstract】[Background] Streptomyces venezuelae Snea253 is an actinomycete with nematicidal activity to plant-parasitic nematode. Bioinformatics analysis showed that γ-aminobutyrate transaminase gene (gabT) is one of the important genes involved in carbon metabolism in Snea253. [Objective] The purpose of this research is to clarify that the gabT gene affects the activity of the strain by regulating the γ-aminobutyric acid metabolic pathway of Snea253. [Methods] First, the mutant strain (Snea253-R) obtained by UV mutation was used to overexpress the gabT gene, and Meloidogyne incognita J2 was used as the target nematode. γ-aminobutyric acid and the metabolite succinic acid were detected by enzyme-linked immunoassay (ELISA) and high performance liquid chromatography (HPLC), and the nematicidal activity was also detected. Meanwhile, the gabT gene expression level, product content and nematicidal activity of wild-type strain with eight carbon sources culture were detected. [Results] The expression of gabT gene in overexpression strain R-pIB139 was up-regulated, γ-aminobutyric acid content was decreased, the content of succinic acid was increased, and the nematicidal activity was increased by 39% in comparison with the control. In eight carbon sources cultures, the carbon source medium with higher relative expression of gabT gene in wild plants was soluble starch and corn starch. Under this condition, γ-aminobutyric acid content in fermentation broth was lower, the other products of metabolism were increased, and the nematicidal activity was higher. [Conclusion] By changing the expression of gabT gene, we confirmed that γ-aminobutyric acid shunt plays an important role in regulating the metabolism of Snea253 to enhance nematicidal activity.

【Keywords】 γ-Aminobutyrate transaminase gene gabT; Streptomyces venezuelae; γ-Aminobutyric acid(GABA); Succinic acid; Nematicidal activity;

【DOI】

【Funds】 National Key Research and Development Program of China (2017YFD0201104) National Natural Science Foundation of China (31471748)

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(Translated by CHEN T)

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This Article

ISSN:0253-2654

CN: 11-1996/Q

Vol 46, No. 12, Pages 3257-3266

December 2019

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion and conclusion
  • References