Characteristics of Distribution of Blood Vessels and Nerve Fibers in the Skin Tissues of Acupoint “Taichong” (LR3) in the Rat

XU Dong-sheng1 SHE Chen1 WANG Jia1 CUI Jing-jing1 CAI Hong1 BAI Wan-zhu1

(1.Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing, China 100700)

【Abstract】Objective To investigate the microstructure (blood vessels and nerve fibers) of the skin tissue in “Taichong” (LR3) region for reveling morphological characteristics of acupoint. Methods Five SD rats were used in the present study. The skin tissue in the region of acupoint LR3 was taken from the dorsum of hind foot following transcardial perfusion with 4% paraformaldehyde. Then, the skin samples were sagittally or horizontally cut into sections (20 μm or 40 μm in the thickness) to be stained with Phalloidin and calcitonin gene related peptide (CGRP) by using fluorescent histochemistry and immunohistochemistry. The labeled vascular structure and nerve fibers were observed and recorded using fluorescent microscope and laser scanning confocal microscope. Results In the skin tissue of LR3, different types of blood vessels labeled by phalliodin, including capillaries and glomera mainly distributing in the superficial layer of the dermis, and thicker blood vessels and their sub-branches mainly existing in the deeper layer of the dermis and the subcutaneous layer, were found. In addition, CGRP positive nerve fibers were found to run parallel to the thin blood vessels or to gather around the thicker blood vessels. From the subcutaneous layer to the epidermis, blood vessels and nerve fibers coexisted and formed a stereo-network structure. Conclusion In the skin of LR3 area, there exists a stereo-network structure consisting of different types of blood vessels and nerve fibers. Although this structure is not specifically associated with acupoint area alone, this result may provide a new sight to further understand the microstructure of acupoint.

【Keywords】 Acupoint; Microstructure; Blood vessel; Nerve fiber; Phalloidin; Calcitonin gene related peptide (CGRP) ;

【DOI】

【Funds】 National Natural Science Foundation of China (81373557 and 81403327) Self-Determination Research Program of Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences (ZZ 16010)

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This Article

ISSN:1000-4289

CN: 11-1299/B

Vol , No. 01, Pages 132-141

February 2019

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Abstract

  • 1 Materials and methods
  • 2 Results
  • 3 Discussions
  • References