The mechanism of tagitinin D-induced apoptosis of HepG2 cells

YANG Shan1 WEI Rui-ran1 CHEN Yuan-zhi2 YANG Xu2 WANG Juan2 YANG Xing-zhi2 HE Zhi-xu1 DING Xiao2

(1.Department of Immunology, Basic Medical College, Tissue Engineering and Stem Cell Research Center, Guizhou Medical University, Guiyang 550004)
(2.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201)

【Abstract】Tagitinin D was identified as one of the main compounds present in plants of Asteraceae, which exhibited anti-tumor activities. To explore the mechanism of HepG2 cell apoptosis induced by tagitinin D, we used colony formation assay to detect the cell proliferation ability. Then, we determined the cell cycle and apoptosis by flow cytometry, the reactive oxygen species (ROS) level by DCFH-DA, and ER stress-related protein expression level by Western blot assay. The results showed that tagitinin D increased ROS levels, inhibited the cell proliferation, arrested the cell cycle in the G2/M phase, and induced apoptosis of HepG2 cells. The expression levels of proteins, such as PDI, Calnexin, Ero1-Lα, Bip, IRE1-Lα, and p-eIF2α, involved in ER stress signaling pathway were up-regulated. In summary, all the data suggested that tagitinin D induced ROS which further elicited the ER stress response and led to apoptosis of HepG2 cells.

【Keywords】 tagitinin D; ROS; apoptosis; ER stress signaling pathway;

【DOI】

【Funds】 National Natural Science Foundation of China (81703393)

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This Article

ISSN:1001-6880

CN: 51-1335/Q

Vol 32, No. 03, Pages 359-364

March 2020

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Article Outline

Abstract

  • 1 Material and instruments
  • 2 Methods
  • 3 Results
  • 4 Discussion and conclusion
  • References