Study on the mechanism of Radix Salviae Miltiorrhizae–Radix Puerariae in treating coronary heart disease based on network pharmacology

MA Yang1,2 WANG Wen-jun1,2 HUANG Shao-jie2 LEI Lu2 WANG Kai1,2 FENG Xiao-na2 DING Yi2 WEN Ai-dong1,2

(1.School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi Province, China 710042)
(2.The First Affiliated Hospital of Air Force Medical University, Xi’an, Shaanxi Province, China 710032)

【Abstract】To explore the mechanism of action of Radix Salviae Miltiorrhizae–Radix Puerariae in treating coronary heart disease (CHD). Based on network pharmacology, the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) was retrieved to collect the active ingredients and targets of Radix Salviae Miltiorrhizae–Radix Puerariae. Based on the TDD, DrugBank and Disgenet databases, the targets of CHD were harvested, and the network pharmacology analysis software and related databases were used for establishing the protein–protein interaction (PPI) network, conducting the target enrichment analysis and constructing the active ingredient–target–pathway network. The results showed that 61 active ingredients and 68 common targets were screened out from Radix Salviae Miltiorrhizae and Radix Puerariae. GO analysis showed that Radix Salviae Miltiorrhizae and Radix Puerariae participated in the transcription and inflammation of RNA polymerase II promoter and other biological processes (BPs). The targets were mainly concentrated in the extracellular space, plasma membrane and nucleus. The results of KEGG analysis showed that the Radix Salviae Miltiorrhizae–Radix Puerariae exerted the effects via the signaling pathways such as HIF-10, PI3 K-Akt, TNF and Jak-STAT. Above all, some active ingredients of Radix Salviae Miltiorrhizae–Radix Puerariae, such as tanshinone ⅡA, mignonette and puerarin, played a role of regulating the blood pressure, angiogenesis and inflammation by affecting the HIF-10, PI3K-Akt, TNF and Jak-STAT signal pathways. These pathways were primarily associated with the common targets of TNF, VEGFA, TP53 and IL6. The results of this study have provided a reference for further study of the pharmacological effects of Radix Salviae Miltiorrhizae–Radix Puerariae.

【Keywords】 network pharmacology; coronary heart disease; Radix Salviae Miltiorrhizae–Radix Puerariae; mechanism of action;

【DOI】

【Funds】 National Natural Science Foundation of China (81603385)

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This Article

ISSN:1001-6880

CN:51-1335/Q

Vol 32, No. 02, Pages 209-223

February 2020

Downloads:13

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results
  • 3 Discussion
  • References