Study on the mechanism of Radix Salviae Miltiorrhizae–Radix Puerariae in treating coronary heart disease based on network pharmacology
(2.The First Affiliated Hospital of Air Force Medical University, Xi’an, Shaanxi Province, China 710032)
【Abstract】To explore the mechanism of action of Radix Salviae Miltiorrhizae–Radix Puerariae in treating coronary heart disease (CHD). Based on network pharmacology, the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) was retrieved to collect the active ingredients and targets of Radix Salviae Miltiorrhizae–Radix Puerariae. Based on the TDD, DrugBank and Disgenet databases, the targets of CHD were harvested, and the network pharmacology analysis software and related databases were used for establishing the protein–protein interaction (PPI) network, conducting the target enrichment analysis and constructing the active ingredient–target–pathway network. The results showed that 61 active ingredients and 68 common targets were screened out from Radix Salviae Miltiorrhizae and Radix Puerariae. GO analysis showed that Radix Salviae Miltiorrhizae and Radix Puerariae participated in the transcription and inflammation of RNA polymerase II promoter and other biological processes (BPs). The targets were mainly concentrated in the extracellular space, plasma membrane and nucleus. The results of KEGG analysis showed that the Radix Salviae Miltiorrhizae–Radix Puerariae exerted the effects via the signaling pathways such as HIF-10, PI3 K-Akt, TNF and Jak-STAT. Above all, some active ingredients of Radix Salviae Miltiorrhizae–Radix Puerariae, such as tanshinone ⅡA, mignonette and puerarin, played a role of regulating the blood pressure, angiogenesis and inflammation by affecting the HIF-10, PI3K-Akt, TNF and Jak-STAT signal pathways. These pathways were primarily associated with the common targets of TNF, VEGFA, TP53 and IL6. The results of this study have provided a reference for further study of the pharmacological effects of Radix Salviae Miltiorrhizae–Radix Puerariae.
【Keywords】 network pharmacology; coronary heart disease; Radix Salviae Miltiorrhizae–Radix Puerariae; mechanism of action;
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