Sequence analysis and expression patterns of histone H2A variants in Phytophthora infestans

Xiaowen Wang1 Liyun Guo1

(1.The Ministry of Agriculture Key Laboratory for Plant Pathology, Department of Plant Pathology, China Agricultural University, Beijing, China 100193)

【Abstract】In eukaryotic cells, DNA is packaged inside the nucleus together with histones to form nucleosomes. Each histone molecule contains two of each core histone subunits H2A, H2B, H3 and H4. Among core histones, the H2A family is of great interest due to the high diversity of specialized variants. These variants have shown important role in critical cellular processes. Epigenetic mechanism in oomycetes is barely known. Phytophthora infestans is a severe pathogen and a model species in oomycetes. In this study, we studied the sequence and expression pattern of H2A variants of P. infestans through genome search, sequences alignment, phylogenetic analysis and realtime qPCR detection. P. infestans contains conserved genes encoding histone variants H2A.X.1, H2A.X.2 and H2A.Z, and these genes have specific expression patterns during development and infection stages. Our datasets provide useful inputs to help explore the epigenetic mechanisms of oomycetes.

【Keywords】 oomycetes; late blight pathogen; H2A.X; H2A.Z; phylogeny; expression pattern;

【DOI】

【Funds】 National Natural Science Foundation of China (No. 30270862) National Natural Science Foundation of China(No.30270862)

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(Translated by FANG Huaming)

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This Article

ISSN:1000-3061

CN: 11-1998/Q

Vol 32, No. 11, Pages 1564-1575

November 2016

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Abstract

  • 1 Materials and methods
  • 2 Results and Analyses
  • 3 Discussion
  • References