Pilot-scale Purification of Lipopeptide from Marine-derived Bacillus marinus

Kangbo Gu1 Cheng Guan1 Jiahui Xu1 Shulan Li2 Yuanchan Luo1 Guomin Shen1 Daojing Zhang1 Yuanguang Li1

(1.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China 200237)
(2.Shanghai Zeyuan Marine Biotechnology Co., Ltd., Shanghai, China 200237)

【Abstract】This research was aimed at establishing the pilot-scale purification technology of lipopeptide from marine-derived Bacillus marinus. We studied lipopeptide surfactivity interferences on scale-up unit technologies including acid precipitation, methanol extraction, solvent precipitation, salting out, extraction, silica gel column chromatography and HZ806 macroporous absorption resin column chromatography. Then, the unit technologies were combined in a certain order, to remove the impurities gradually, and to gain purified lipopeptide finally, with high recovery rate throughout the whole process. The novel pilot-scale purification technology could effectively isolate and purify lipopeptide with 87.51% to 100% purity in hectograms from 1 ton of Bacillus marinus B-9987 fermentation broth with more than 81.73% recovery rate. The first practical hectogram production of highly purified lipopeptide derived from Bacillus marinus was achieved. With this new purification method, using complex media became possible in fermentation process to reduce the fermentation cost and scale-up the purification for lipopeptide production. For practicability and economy, foaming problem resulting from massive water evaporation was avoided in this technology.

【Keywords】 Bacillus marinus; lipopeptide; pilot-scale purification; hectogram; scale-up;


【Funds】 National Key Technology Research and Development Program of the Ministry of Science and Technology of China (No. 2011BAE06B04-16) Key Technologies Research and Development Program of China(No.2011BAE06B04-16)

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(Translated by SONG Junfeng)


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This Article


CN: 11-1998/Q

Vol 32, No. 11, Pages 1549-1563

November 2016


Article Outline



  • 1 Materials and methods
  • 2 Results and Analysis
  • 3 Discussion
  • References