Delivery of recombinant enhanced green fluorescent protein to Pichia pastoris cell wall directed by a mammalian nonclassical secretion signal peptide

Qin Yufeng 1 Chen Yaosheng 1 Liu Zhiguo1 Zhang Ying 1 Liu Hailong 1 He Zuyong 1

(1.State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China 510006)

【Abstract】A mammalian nonclassical secretion sequence derived from mouse Engrailed 2 homeoprotein (En2) was used to direct the secretion of the enhanced green fluorescent protein from Pichia pastoris. This signal peptide conferred the transport of enhanced green fluorescent protein into periplasm through an endoplasmic reticulum-golgi independent pathway, without inducing severe unfolded protein response as compared with Saccharomyces cerevisae α-factor preprosequence. This study implies that this mammalian nonclassical signal peptide could be developed as a useful tool for delivering cargoes to the cell surface of yeast.

【Keywords】 ER stress; nonclassical secretion; Pichia pastoris; qPCR;

【DOI】

【Funds】 National Transgenic Major Program (No. 2016ZX08006003-006) National Transgenic Major Program(No.2016ZX08006003-006) Natural Science Foundation of Guangdong Province (No. 2016A030313310) Natural Science Foundation of Guangdong Province(No.2016A030313310)

Download this article

    References

This Article

ISSN:1000-3061

CN: 11-1998/Q

Vol 32, No. 10, Pages 1455-1464

October 2016

Downloads:1

Share
Article Outline

Knowledge

Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion
  • References