Delivery of recombinant enhanced green fluorescent protein to Pichia pastoris cell wall directed by a mammalian nonclassical secretion signal peptide
【Abstract】A mammalian nonclassical secretion sequence derived from mouse Engrailed 2 homeoprotein (En2) was used to direct the secretion of the enhanced green fluorescent protein from Pichia pastoris. This signal peptide conferred the transport of enhanced green fluorescent protein into periplasm through an endoplasmic reticulum-golgi independent pathway, without inducing severe unfolded protein response as compared with Saccharomyces cerevisae α-factor preprosequence. This study implies that this mammalian nonclassical signal peptide could be developed as a useful tool for delivering cargoes to the cell surface of yeast.
【Keywords】 ER stress; nonclassical secretion; Pichia pastoris; qPCR;