利用糖芯片技术检测氨基糖苷类抗生素与RNAs和蛋白质之间的相互作用
(2.江南大学无锡医学院, 江苏无锡 214122)
【摘要】氨基糖苷类抗生素是一类广谱型抗细菌感染药物,其不断增加的细菌耐药性很大程度上限制了它的临床应用,研究和开发新型氨基糖苷类抗生素具有重要意义。将氨基糖苷类抗生素固定到玻璃片基上,制成糖芯片,再分别与荧光标记的RNAs和蛋白质杂交,通过分析杂交后的荧光信号强度检测它们之间的相互作用。结果显示,氨基糖苷类抗生素芯片可以特异性地与r RNA的A位点模拟物、I型核酶和蛋白酶结合。因此糖芯片技术不仅可以检测氨基糖苷类抗生素与r RNAs的特异性结合,而且可以应用于寻找新型RNA结合配体的研究,为快速鉴定和筛选可紧密结合RNA靶标且毒性较低的新型氨基糖苷类抗生素奠定了一定的基础。
【关键词】 氨基糖苷类抗生素芯片; rRNA的A位点模拟物; I型核酶; Klenow DNA聚合酶; 磷脂酶C;
【DOI】
【基金资助】
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ISSN:1000-3061
CN: 11-1998/Q
Vol 32, No. 10, Pages 1362-1371
October 2016
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