Isolation, identification and structural characterization of secondary metabolites from amarine sponge-derived rare actinobacterium Dermacoccus sp. X4

Yanfeng Zhang1,2 Yong Xu1,2 Lei Chen1,2 Jun Hu1,2 Xuecheng Zhang1,2 Wei Fang1,2 Zemin Fang1,2 Yazhong Xiao1,2

(1.School of Life Science, Anhui University, Hefei, Anhui, China 230601)
(2.Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui, China 230039)
【Knowledge Link】polymer

【Abstract】We isolated and identified the symbiotic and adnascent microorganisms from an unidentified sponge collected from 10-meter-deep seawater of the Xisha Islands in China. A total of 16 strains were obtained and identified. Through bacteriostatic activity assay, one of the strains, Dermacoccus sp. X4, was found to effectively inhibit the growth of Staphylococcus aureus. Subsequently, its secondary metabolites were purified by silica gel partition, octadecylsilane (ODS) reverse phase, Sephadex TMLH-20 size exclusion, and C18 reverse phase chromatography. Using liquid chromatography, mass spectrometry, and nuclear magnetic resonance, three of the purified compounds were structurally characterized to be one 3-(4-hydroxybenzyl) hexahydropyrrolo [1, 2-a]pyrazine-1, 4-dione and two indole acid glycerides. This is the first report about indole acid glyceride isolated from microbial secondary metabolites, enriching marine drug candidate resources.

【Keywords】 sponge associated microorganism; secondary metabolite; bacteriostatic activity; isolation and purification; structural characterization;

【DOI】

【Funds】 National High Technology Research and Development Program of China (863 Program) (No. 2013AA092901) National High Technology Research and Development Program of China(863 Program)(No.2013AA092901) Scientific Research Foundation for Returned Scholars, Ministry of Education of China Scientific Research Foundation for Returned Scholars,Ministry of Education of China the Introduction Project of Academic and Technology Leaders in Anhui University (No. 32030066); Innovative Research Team Program of 211 Project in Anhui University the Introduction Project of Academic and Technology Leaders in Anhui University(No.32030066),Innovative Research Team Program of 211 Project in Anhui University

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(Translated by ZHAO Bo)

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This Article

ISSN:0438-1157

CN: 11-1946/TQ

Vol 70, No. 01, Pages 271-279

January 2019

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Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion
  • References