华美奥锹甲不同颚型雄性成虫肠道细菌群落多样性

蒋宇1 孙丙华1 曹玉言1 翟勇宁1 万霞1

(1.安徽大学资源与环境工程学院生态学系, 合肥 230601)

【摘要】【目的】分析比较华美奥锹甲Odontolabis fallaciosa不同颚型雄性成虫肠道细菌的群落结构和多样性。【方法】分别提取华美奥锹甲雄性3种颚型(大颚型、中颚型和小颚型)31头成虫样本(15个大颚型、10个中颚型、6个小颚型)的肠道DNA,利用Illumina Mi Seq技术对其肠道细菌的16S r DNA V3-V4变异区序列进行测序,统计样品操作分类单元(OTU)数量,分析物种组成丰度、α多样性和β多样性。【结果】共获得优质序列2 238 637条,在97%相似度下可将其聚类为用于物种分类的3 256个OTU,共注释到的主要分类阶元有42个门、328个科和542个属。门分类水平上,变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、柔膜菌门(Tenericutes)为优势门类;属分类水平上,优势属为不动杆菌属Acinetobacter、Dysgonomonas属、巴尔通氏体属Bartonella、金黄杆菌属Chryseobacterium。α多样性分析结果表明,华美奥锹甲雄性成虫肠道细菌群落整体多样性比较丰富;β多样性分析结果表明,大颚型、中颚型和小颚型之间OTU组成有差异。【结论】采用Illumina Mi Seq测序技术,分析了华美奥锹甲雄性成虫肠道细菌群落的结构和组成。大颚型与中颚型雄性成虫间的肠道细菌群落组成差异显著,但大颚型与小颚型间、中颚型与小颚型间雄性成虫肠道细菌群落组成无差异。

【关键词】 华美奥锹甲; 肠道细菌; 16S rDNA; 细菌群落组成; 群落多样性;

【DOI】

【基金资助】 国家自然科学基金项目(31201745,31071954,31572311)

Download this article

    References

    Berry D,2016.The emerging view of Firmicutes as key fibre degraders in the human gut.Environ.Microbiol.,18(7):2081-2083.

    Briones-Roblero CI,Rodríguez-Díaz R,Santiago-Cruz JA,Zú1iga G,Rivera-Ordu1a FN,2016.Degradation capacities of bacteria and yeasts isolated from the gut of Dendroctonus rhizophagus(Curculionidae:Scolytinae).Folia Microbiol.,62(1):1-9.

    Brucker RM,Bordenstein SR,2012.Speciation by symbiosis:trends in ecology&evolution.Trends Ecol.Evol.,27(8):443-451.

    Cejanavarro JA,Nguyen NH,Karaoz U,Gross SR,Herman DJ,Andersen GL,Bruns TD,Pett-Ridge J,Blackwell M,Brodie EL,2014.Compartmentalized microbial composition,oxygen gradients and nitrogen fixation in the gut of Odontotaenius disjunctus.ISMEJ.,8(1):6-18.

    Chen BS,Lu XM,Shao YQ,2017.Diversity of the gut microbiota in lepidopteran insects and their interaction with hosts.Acta Entomol.Sin.,60(6):710-722.

    Colman DR,Toolson EC,Takacsvesbach CD,2012.Do diet and taxonomy influence insect gut bacterial communities?Mol.Ecol.,21(20):5124-5137.

    Douglas AE,2009.The microbial dimension in insect nutritional ecology.Funct.Ecol.,23(1):38-47.

    Douglas AE,2015.Multiorganismal insects:diversity and function of resident microorganisms.Annu.Rev.Entomol.,60(1):17-34.

    Du L,Liu F,Zhao P,Zhao T,Doyle MP,2017.Characterization of Enterococcus durans 152 bacteriocins and their inhibition of Listeria monocytogenes in ham.Food Microbiol.,68:97-103.

    Engel P,Moran NA,2013a.The gut microbiota of insects-diversity in structure and function.FEMS Microbiol.Rev.,37(5):699-735.

    Engel P,Moran NA,2013b.Functional and evolutionary insights into the simple yet specific gut microbiota of the honey bee from metagenomic analysis.Gut Microbes,4(1):60-65.

    Evans JD,Lopez DL,2004.Bacterial probiotics induce an immune response in the honey bee(Hymenoptera:Apidae).J.Econ.Entomol.,97(3):752-756.

    Franzini PZN,Jean-Baptiste R,Scholtz CH,Sole CL,Sandra R,Cowan DA,2016.The gut microbiomes of two Pachysoma Macleay desert dung beetle species(Coleoptera:Scarabaeidae:Scarabaeinae)feeding on different diets.PLo S ONE,11(8):e0161118.

    Geib SM,Jimenez-Gasco MM,Carlson JE,Tie M,Hoover K,2009.Effect of host tree species on cellulase activity and bacterial community composition in the gut of larval Asian longhorned beetle.Environ.Entomol.,38(3):686-699.

    Genta FA,Dillon RJ,Terra WR,Ferreira C,2006.Potential role for gut microbiota in cell wall digestion and glucoside detoxification in Tenebrio molitor larvae.J.Insect Physiol.,52(6):593-601.

    Gotoh H,Miyakawa H,Ishikawa A,Ishikawa Y,Sugime Y,Emlen DJ,Lavine LC,Miura T,2014.Developmental link between sex and nutrition;doublesex regulates sex-specific mandible growth via juvenile hormone signaling in stag beetles.PLo S Genet.,10(1):e1004098.

    Goyens J,Soons J,Aerts P,Dirckx J,2014.Finite-element modelling reveals force modulation of jaw adductors in stag beetles.J.R.Soc.Interface,11(101):20140908.

    Guo J,Wu J,Deng XY,Lin LB,Liu S,Li JL,2015.Advances in research on insect gut microbiota and their functions.J.Appl.Entomol.,52(6):1345-1352.

    Hamdi C,Balloi A,Essanaa J,Crotti E,Gonella E,Raddadi N,Ricci I,Boudabous A,Borin S,Manino A,Bandi C,Alma A,Daffonchio D,Cherif A,2011.Gut microbiome dysbiosis and honeybee health.J.Appl.Entomol.,135(7):524-533.

    Handique G,Phukan A,Bhattacharyya B,Baruah AA,Rahman SW,Baruah R,2017.Characterization of cellulose degrading bacteria from the larval gut of the white grub beetle Lepidiota mansueta(Coleoptera:Scarabaeidae).Arch.Insect Biochem.Physiol.,94(2):e21370.

    Harvey DJ,Gange AC,Hawes CJ,Rink M,2011.Bionomics and distribution of the stag beetle,Lucanus cervus(L.)across Europe.Insect Conserv.Diver.,4(1):23-38.

    Hongoh Y,Deevong P,Inoue T,Moriya S,Trakulnaleamsai S,Ohkuma M,Vongkaluang C,Noparatnaraporn N,Kudo T,2005.Intra-and interspecific comparisons of bacterial diversity and community structure support coevolution of gut microbiota and termite host.Appl.Environ.Microb.,71(11):6590-6599.

    Hu X,2014.Gut-associated Microbiota Diversity of the White Pine Beetle(Dendroctonus armandi)and Cellulolytic Microbial Community in Its Larval Gut.Ph D Dissertation,Northwest A&FUniversity,Yangling,Shaanxi.

    Ikedaohtsubo W,Desai M,Stingl U,Brune A,2007.Phylogenetic diversity of‘endomicrobia’and their specific affiliation with termite gut flagellates.Microbiology,153(10):3458-3465.

    Kim JM,Choi MY,Kim JW,Lee SA,Ahn JH,Song J,Kim SH,Weon HY,2017.Effects of diet type,developmental stage,and gut compartment in the gut bacterial communities of two Cerambycidae species(Coleoptera).J.Microbiol.,55(1):21-30.

    Liu XG,Yang YJ,Liao QJ,Xu HX,Liu YH,Lu ZX,2016.Analysis of the bacterial community structure and diversity in the intestine of Cnaphalocrocis medinalis(Lepidoptera:Pyralidae).Acta Entomol.Sin.,59(9):965-976.

    Martínez-Falcón AP,Durbán A,Latorre A,Antón J,2011.Bacteria associated with Copestylum(Diptera,Syrphidae)larvae and their cactus host Isolatocereus dumortieri.PLo S ONE,6(11):e27443.

    Mason CJ,Lowe-Power TM,Rubert-Nason KF,Lindroth RL,Raffa KF,2016.Interactions between bacteria and aspen defense chemicals at the phyllosphere-herbivore interface.J.Chem.Ecol.,42(3):193-201.

    Montagna M,Chouaia B,Mazza G,Prosdocimi EM,Crotti E,Mereghetti V,Vacchini V,Giorgi A,Biase AD,Longo S,Cervo R,Lozzia GC,Alma A,Bandi C,Daffonchioet D,2015.Effects of the diet on the microbiota of the red palm weevil(Coleoptera:Dryophthoridae).PLo S ONE,10(1):e0117439.

    Neuvonen MM,Tamarit D,Näslund K,Liebig J,Feldhaar H,Moran NA,Guy L,Andersson SG,2016.The genome of Rhizobiales bacteria in predatory ants reveals urease gene functions but no genes for nitrogen fixation.Sci.Rep.,6:39197.

    Rowland JM,Emlen DJ,2009.Two thresholds,three male forms result in facultative male thrimorphism in beetles.Science,323(5915):773-776.

    Salmassi TM,Leadbetter JR,2003.Analysis of genes of tetrahydrofolatedependent metabolism from cultivated spirochaetes and the gut community of the termite Zootermopsis angusticollis.Microbiology,149(9):2529-2537.

    Schmitt-Wagner D,Friedrich MW,Wagner B,Brune A,2003.Phylogenetic diversity,abundance,and axial distribution of bacteria in the intestinal tract of two soil-feeding termites(Cubitermes spp.).Appl.Environ.Microb.,69(10):6007-6017.

    Segers FH,Ke2nerováL,Kosoy M,Engel P,2017.Genomic changes associated with the evolutionary transition of an insect gut symbiont into a blood-borne pathogen.ISME J.,11(5):1232-1244.

    Sheng P,2014.Diversities of Gut Microbes and Glycoside Hydrolases in Different Developmental Stages of Holotrichia parallela.Ph DDissertation.Huazhong Agricultural University,Wuhan.

    Shil RK,Mojumder S,Sadida FF,Uddin M,Sikdar D,2014.Isolation and identification of cellulolytic bacteria from the gut of three phytophagus insect species.Braz.Arch.Biol.Technol.,57:927-932.

    Shu RH,Lu SL,Xu XL,2011.Study on biogenic amines interaction by Enterococcus faecalis and Enterococcus faecium.Food Fermn.Ind.,37(6):16-20.

    Sun XX,Ning N,Tan HJ,NI JF,2017.Research progress of diversity and function of symbiotic microbes in the gut of termites.Chin.J.Appl.Environ.Biol.,23(4):764-770.

    Suzuki K,Sakamoto H,Shinozaki Y,Tabata J,Watanabe T,Mochizuki A,Koitabashi M,Fujii T,Tsushima S,Kitamoto HK,2013.Affinity purification and characterization of a biodegradable plasticdegrading enzyme from a yeast isolated from the larval midgut of a stag beetle,Aegus laevicollis.Appl.Microbiol.Biot.,97(17):7679-7688.

    Tanahashi M,Kubota K,2013.Utilization of the nutrients in the soluble and insoluble fractions of fungal mycelium by larvae of the stag beetle,Dorcus rectus(Coleoptera:Lucanidae).Eur.J.Entomol.,110(4):611-615.

    Tanahashi M,Kubota K,Matsushita N,Togashi K,2010.Discovery of mycangia and the associated xylose-fermenting yeasts in stag beetles(Coleoptera:Lucanidae).Naturwissenschaften,97(3):311-317.

    Thomaes A,Kervyn T,Maes D,2008.Applying species distribution modelling for the conservation of the threatened saproxylic stag beetle(Lucanus cervus).Biol.Conserv.,141(5):1400-1410.

    Tian XY,Song FP,Zhang J,Liu RM,Zhang XP,Duan JY,Shu CL,2017.Diversity of gut bacteria in larval Protaetia brevitarsis(Coleoptera:Scarabaedia)fed on corn stalk.Acta Entomol.Sin.,60(6):632-641.

    Turnbaugh PJ,Bäckhed F,Fulton L,Gordon JI,2008.Marked alterations in the distal gut microbiome linked to diet-induced obesity.Cell Host Microbe,3(4):213.

    Ulyshen MD,Zachos LG,Stireman JO,Sheehan TN,Garrick RC,2017.Insights into the ecology,genetics and distribution of Lucanus elaphus Fabricius(Coleoptera:Lucanidae),North America’s giant stag beetle.Insect Conserv.Divers.,10(4):331-340.

    Wang J,Hui C,Ming T,2017.Community structure of gut bacteria of Dendroctonus armandi(Coleoptera:Curculionidae:Scolytinae)larvae during overwintering stage.Sci.Rep.,7(1):14242.

    Wang Y,Sheng HF,He Y,Wu JY,Jiang YX,Tam NF,Zhou HW,2012.Comparison of the levels of bacterial diversity in freshwater,intertidal wetland,and marine sediments by using millions of Illumina tags.Appl.Environ.Microb.,78(23):8264-8271.

    Werren JH,Windsor DM,2000.Wolbachia infection frequencies in insects:evidence of a global equilibrium?Proc.Biol.Sci.,267(1450):1277-1285.

    Wong AC,Chaston JM,Douglas AE,2013.The inconstant gut microbiota of Drosophila species revealed by 16S rRNA gene analysis.ISME J.,7(10):1922-1932.

    Xiang H,Huang YP,2008.Symbiosis between gut microbiota and insects.Chin.Bull.Entomol.,45(5):687-693.

    Xiang XJ,He D,He JS,Myrold DD,Chu H,2017.Ammonia-oxidizing bacteria rather than archaea respond to short-term urea amendment in an alpine grassland.Soil Biol.Biochem.,107:218-225.

    Yang H,2004.Structure and Diversity of the Bacterial Community in the Gut of Wood-Feeding Lower Termites(Reticulitermes spp.).Ph DDissertation,Huazhong Agricultural University,Wuhan.

    Yun JH,Roh SW,Whon TW,Jung MJ,Kim MS,Park DS,Yoon C,Nam YD,Kim YJ,Choi JH,Kim JY,Shin NR,Kim SH,Lee WJ,Bae JW,2014.Insect gut bacterial diversity determined by environmental habitat,diet,developmental stage,and phylogeny of host.Appl.Environ.Microb.,80(17):5254-5264.

    Zhang W,Zheng Y,Wang YF,2017.Effects of Wolbachia infection on the expression of DNA 6m A demethylase gene DMAD in gonads and early embryos of Drosophila melanogaster.Acta Entomol.Sin.,60(11):1292-1299.

This Article

ISSN:0454-6296

CN: 11-1832/Q

Vol 61, No. 03, Pages 322-330

March 2018

Downloads:0

Share
Article Outline

摘要

  • 1 材料与方法
  • 2 结果
  • 3 讨论
  • 参考文献