Large Field-of-View and Deep Tissue Optical microscopy Based on Parallel Wavefront Correction Algorithm
(2.Center for Neuroscience, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China 310058)
【Abstract】As for deep tissues, the field of view of one-time correction in the widely used adaptive optics is limited and the refresh rate of a spatial light modulator or a deformable mirror is also limited. Therefore, it is difficult for them to satisfy the requirement of large field-of-view (FOV) rapid correction of wavefront distortion and thus that of high-speed imaging. A parallel wavefront correction method is proposed based on the conjugate adaptive optics correction system and the coherent optical adaptive technique. In this method, without increasing the number of refresh times of spatial light modulator, the large FOV of one-time correction can be realized by means of the parallel measurement of wavefront distortion of multiple guide stars, which provides a feasible reference solution for the high-speed and high-resolution imaging of deep tissues. The simulation results show that when 9 guiding stars are used, the effective FOV of one-time correction by the proposed method is about 4.7 times that of the conventional method for a thin scattering medium composed of 5 layers of random phasemasks, and 4.6 times that of the conventional method for 120-μm thick mouse brain tissue. Moreover, the proposed method can further improve the FOV of one-time correction by increasing the number of guide stars while the correction time does not significantly increase, which has broad application prospect in the large FOV imaging of in vivo biological tissues.
【Keywords】 imaging systems; microscopy; deep tissue imaging; scattering measurement; adaptive optics; wavefront shaping;
(Translated by LIU T)
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