Optimization of the Mainstream Anaerobic Ammonium Oxidation Process and Its Changes of the Microbial Community

FU Kun-ming1 FU Chao1 LI Hui1 JIANG Shan1 QIU Fu-guo1 CAO Xiu-qin1

(1.Sino-Dutch R&D Center for Future Wastewater Treatment, Key Laboratory of Urban Storm Water System and Water Environment, School of Environment and Energy Engineering, Beijing University of Civil Engineering and Architecture, Beijing, China 100044)

【Abstract】The completely autotrophic nitrogen removal over nitrite (CANON) biofilm reactor acclimated by high-strength ammonia wastewater was used to treat low-strength ammonia wastewater. The treatment can be divided into three stages: (1) the nitrogen removal efficiency of anaerobic ammonia oxidation was low during the continuous aeration stage with inorganic wastewater as raw water (day 0 to day 59) and with an aeration amount of 30 mL·min−1 and ammonia concentration of 80 mg·L−1 (until day 56), and the TN removal load was only 0.13 kg·(m3·d)−1; (2) during the continuous aeration stage with domestic wastewater as raw water (day 60 to day 110), the addition of organic carbon improved the TN removal load to 0.22 kg·(m3·d)−1 on day 79; the removal rate of NH4+–N then reached 100% when the aeration volume improved to 100 mL·min−1 on day 103; however, the TN removal efficiency and TN removal load decreased to 42.36% and 0.14 kg·(m3·d)−1, respectively. (3) To increase both the NH4+–N and TN removal efficiency during the intermittent aeration stage with domestic wastewater as raw water (day 110 to day 160), the aeration amount was increased to 50 mL·min−1, while aeration was continued for 30 min and was stopped for the next 30 min; on day 131, the NH4+–N removal efficiency increased to 86.34%, and the TN removal efficiency and removal load reached 85.87% and 0.3 kg·(m3·d)−1, respectively; on day 141, the aeration was increased to 100 mL·min−1 and the removal efficiency of NH4+–N reached 100%, while the removal efficiency and removal load of TN were 64.28% and 0.22 kg·(m3·d)−1, respectively, indicating that the intermittent aeration strategy effectively improved the nitrogen removal performance of the CANON reactor. To analyze the variation of the microbial community during different stages, the samples of three stages (day 0, day 56, and day 152) were analyzed using high-throughput sequencing technology. The results show that: (1) Candidatus Brocadia was less affected than Candidatus Kuenenia during the low-strength ammonia stages with inorganic and domestic wastewater as raw water; (2) Nitrosomonas and Nitrospira were the dominant bacteria of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB), respectively. Domestic wastewater had a greater impact on Nitrosomonas than on Nitrospira; (3) denitrifying bacteria were present during the whole stage; Pseudomonas and Paracoccus were the most adaptable, even though their relative abundance during each stage was below 0.5%.

【Keywords】 completely autotrophic ammonium removal over nitrite (CANON) ; domestic wastewater; intermittent aeration; anaerobic ammonium oxidation (ANAMMOX) ; denitrifying bacteria;


【Funds】 Fundamental Research Funds for Universities of Beijing Municipality, Beijing University of Civil Engineering and Architecture (X18214) Science and Technology Development Plan Project of Beijing Municipal Education Commission (SQKM201710016006)

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(Translated by REN XF)


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This Article


CN: 11-1895/X

Vol 39, No. 12, Pages 5596-5604

December 2018


Article Outline


  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion
  • 4 Conclusions
  • References