Optimization of the Mainstream Anaerobic Ammonium Oxidation Process and Its Changes of the Microbial Community

FU Kun-ming1 FU Chao1 LI Hui1 JIANG Shan1 QIU Fu-guo1 CAO Xiu-qin1

(1.Sino-Dutch R&D Center for Future Wastewater Treatment, Key Laboratory of Urban Storm Water System and Water Environment, School of Environment and Energy Engineering, Beijing University of Civil Engineering and Architecture, Beijing, China 100044)

【Abstract】The completely autotrophic nitrogen removal over nitrite (CANON) biofilm reactor acclimated by high-strength ammonia wastewater was used to treat low-strength ammonia wastewater. The treatment can be divided into three stages: (1) the nitrogen removal efficiency of anaerobic ammonia oxidation was low during the continuous aeration stage with inorganic wastewater as raw water (day 0 to day 59) and with an aeration amount of 30 mL·min−1 and ammonia concentration of 80 mg·L−1 (until day 56), and the TN removal load was only 0.13 kg·(m3·d)−1; (2) during the continuous aeration stage with domestic wastewater as raw water (day 60 to day 110), the addition of organic carbon improved the TN removal load to 0.22 kg·(m3·d)−1 on day 79; the removal rate of NH4+–N then reached 100% when the aeration volume improved to 100 mL·min−1 on day 103; however, the TN removal efficiency and TN removal load decreased to 42.36% and 0.14 kg·(m3·d)−1, respectively. (3) To increase both the NH4+–N and TN removal efficiency during the intermittent aeration stage with domestic wastewater as raw water (day 110 to day 160), the aeration amount was increased to 50 mL·min−1, while aeration was continued for 30 min and was stopped for the next 30 min; on day 131, the NH4+–N removal efficiency increased to 86.34%, and the TN removal efficiency and removal load reached 85.87% and 0.3 kg·(m3·d)−1, respectively; on day 141, the aeration was increased to 100 mL·min−1 and the removal efficiency of NH4+–N reached 100%, while the removal efficiency and removal load of TN were 64.28% and 0.22 kg·(m3·d)−1, respectively, indicating that the intermittent aeration strategy effectively improved the nitrogen removal performance of the CANON reactor. To analyze the variation of the microbial community during different stages, the samples of three stages (day 0, day 56, and day 152) were analyzed using high-throughput sequencing technology. The results show that: (1) Candidatus Brocadia was less affected than Candidatus Kuenenia during the low-strength ammonia stages with inorganic and domestic wastewater as raw water; (2) Nitrosomonas and Nitrospira were the dominant bacteria of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB), respectively. Domestic wastewater had a greater impact on Nitrosomonas than on Nitrospira; (3) denitrifying bacteria were present during the whole stage; Pseudomonas and Paracoccus were the most adaptable, even though their relative abundance during each stage was below 0.5%.

【Keywords】 completely autotrophic ammonium removal over nitrite (CANON) ; domestic wastewater; intermittent aeration; anaerobic ammonium oxidation (ANAMMOX) ; denitrifying bacteria;

【DOI】

【Funds】 Fundamental Research Funds for Universities of Beijing Municipality, Beijing University of Civil Engineering and Architecture (X18214) Science and Technology Development Plan Project of Beijing Municipal Education Commission (SQKM201710016006)

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(Translated by REN XF)

    References

    [1] Siegrist H, Salzgeber D, Eugster J, et al. Anammox brings WWTP closer to energy autarky due to increased biogas production and reduced aeration energy for N-removal [J]. Water Science & Technology, 2008, 57(3): 383–388.

    [2] Fu KM, Qiu FG, Zuo ZR. Prospective analysis of application of anaerobic ammonia oxidation technology to municipal wastewater treatment [J]. China Water & Wastewater, 2015, 31(4): 8–13 (in Chinese).

    [3] Lackner S, Gilbert EM, Vlaeminck SE, et al. Full-scale partial nitritation/anammox experiences-an application survey [J]. Water Research, 2014, 55: 292–303.

    [4] Scherson Y, Wells G, Criddle CA, et al. Shortcut nitrogen removal—nitrite shunt and deammonification [M]. Alexandria, Virginia, USA: Water Environment Federation, 2015.

    [5] Cao YS, Hong KB, Van Loosdrecht MCM, et al. Mainstream partial nitritation and anammox in a 200,000 m3/day activated sludge process in Singapore: scale-down by using laboratory fedbatch reactor [J]. Water Science & Technology, 2016, 74(1): 48–56.

    [6] Cao YS, Hong KB, Zhou Y, et al. Mainstream partial nitritation/Anammox nitrogen removal process in the Largest Water Reclamation Plant in Singapore [J]. Journal of Beijing University of Technology, 2015, 41(10): 1441–1454 (in Chinese).

    [7] Cao YS, Van Loosdrecht MCM, Daigger GT. Mainstream partial nitritation-anammox in municipal wastewater treatment:status, bottlenecks, and further studies [J]. Applied Microbiology and Biotechnology, 2017, 101(4): 1365–1383.

    [8] Jenni S, Vlaeminck SE, Morgenroth E, et al. Successful application of nitritation/anammox to wastewater with elevated organic carbon to ammonia ratios [J]. Water Research, 2014, 49: 316–326.

    [9] Cao SB, Wang SY, Wu CC, et al. Shock effect of organic matters on anaerobic ammonia oxidation system [J]. China Environmental Science, 2013, 33(12): 2164–2169 (in Chinese).

    [10] Han XY, Zhang SJ, Gan YP, et al. Start up and maintain of nitritation by the Inhibition of FA and FNA [J]. Environmental Science, 2009, 30(3): 809–814 (in Chinese).

    [11] Chandran K, Smets BF. Single-step nitrification models erroneously describe batch ammonia oxidation profiles when nitrite oxidation becomes rate limiting [J]. Biotechnology and Bioengineering, 2000, 68(4): 396–406.

    [12] Wang HF. Research on N2O emission during completelyautotrophic nitrogen removal over nitrite process [D]. Beijing: Beijing University of Civil Engineering and Architecture, 2016 (in Chinese).

    [13] Environmental Protection Administration of China. 水和废水监测分析方法 [M]. (4th ed.). Beijing: China Environmental Science Press, 2002, 258–415 (in Chinese).

    [14] Fu KM, Zhang J, Cao XS, et al. Effect of aeration rate on CANON reactors with different carriers [J]. CIESC Journal, 2010, 61(2): 496–503 (in Chinese).

    [15] Anthonisen AC, Loehr RC, Prakasam TBS, et al. Inhibition of nitrification by ammonia and nitrous acid [J]. Journal (Water Pollution Control Federation), 1976, 48(5): 835–852.

    [16] Vadivelu VM, Keller J, Yuan ZG. Effect of free ammonia on the respiration and growth processes of an enriched Nitrobacter culture [J]. Water Research, 2007, 41(4): 826–834.

    [17] Han XY, Sun YF, Zhang SJ, et al. Effect of influent ammonia concentration on CANON process for two sludge systems [J]. Journal of Harbin Institute of Technology, 2018, 50(2): 40–45 (in Chinese).

    [18] Ji M, Liu LJ, Zhai HY, et al. Mechanism for effects of high free ammonia loadings on biological nitrification [J]. Environmental Science, 2017, 38(1): 260–268 (in Chinese).

    [19] Sun HW, You YJ, Zhao HN, et al. Inhibitory effect of free ammonia on the activity of nitrifying bacteria and recoverability [J]. China Environmental Science, 2015, 35 (1): 95–100 (in Chinese).

    [20] Zheng ZM, Li J, Ma J, et al. Nitrogen removal via simultaneous partial nitrification, anammox and denitrification (SNAD) process under high DO condition [J]. Biodegradation, 2016, 27(4–6): 195–208.

    [21] Van Der Star WRL, Miclea AI, Van Dongen UGJM, et al. The membrane bioreactor: a novel tool to grow anammox bacteria as free cells [J]. Biotechnology and Bioengineering, 2008, 101(2): 286–294.

    [22] Burrell PC, Keller J, Blackall LL. Microbiology of a nitriteoxidizing bioreactor [J]. Applied and Environmental Microbiology, 1998, 64(5): 1878–1883.

    [23] Daims H, Nielsen PH, Nielsen JL, et al. Novel Nitrospira-like bacteria as dominant nitrite-oxidizers in biofilms from wastewater treatment plants: diversity and in situ physiology [J]. Water Science & Technology, 2000, 41(4–5): 85–90.

    [24] Kornaros M, Dokianakis SN, Lyberatos G. Partial nitrification/denitrification can be attributed to the slow response of nitrite oxidizing bacteria to periodic anoxic disturbances [J]. Environmental Science & Technology, 2010, 44(19): 7245–7253.

    [25] Bao P, Wang SY, Ma B, et al. Effect of dissolve oxygen on the microbial community of the nitrite-oxidizing bacteria in an intermittent aeration REACTOR [J]. China Environmental Science, 2016, 36(9): 2696–2702 (in Chinese).

    [26] Zhang Q, Wang SY, Miao YY, et al. Start-up of CANON process on domestic wastewater using intermittent aeration with low DO [J]. CIESC Journal, 2017, 68(1): 289–296 (in Chinese).

    [27] Bournazou MNC, Hooshiar K, Arellano-Garcia H, et al. Model based optimization of the intermittent aeration profile for SBRs under partial nitrification [J]. Water Research, 2013, 47(10): 3399–3410.

    [28] Zheng ZM, Li J, Yang JY, et al. Nitrogen removal performance of the SNAD process under different intermittent aerobic conditions [J]. China Environmental Science, 2017, 37(2): 511–519 (in Chinese).

    [29] Fu KM, Zhou HT, Su XY, et al. Short-cut nitrification recovery and its transformation into CANON Process in a biofilm reactor [J]. Environmental Science, 2017, 38(4): 1536–1543 (in Chinese).

    [30] Wang SY. Study on nitrogen removal enhancement and microbial characteristics of anammox in low-ammonia sewage treatment [D]. Harbin: Harbin Institute of Technology, 2016 (in Chinese).

    [31] Mi WX, Zhao JQ, Ding XQ, et al. Treatment performance, nitrous oxide production and microbial community under lowammonium wastewater in a CANON process [J]. Water Science & Technology, 2017, 76(12): 3468–3477.

    [32] Ma B. Nitritation and anammox achieved in continous reactors treating sewage [D]. Harbin: Harbin Institute of Technology, 2012 (in Chinese).

    [33] Gao JF, Li T, Fan XY, et al. Abundance and diversity of four nitrogen removal microbes in completely autotrophic nitrogen removal over nitrite (CANON) systems [J]. Journal of Basic Science and Engineering, 2016, 24(5): 863–877 (in Chinese).

    [34] Van Der Star WRL, Abma WR, Blommers D, et al. Startup of reactors for anoxic ammonium oxidation: experiences from the first full-scale anammox reactor in Rotterdam [J]. Water Research, 2007, 41(18): 4149–4163.

    [35] Liu T, Li D, Zeng HP, et al. Biodiversity and quantification of functional bacteria in completely autotrophic nitrogen-removal over nitrite (CANON) process [J]. Bioresource Technology, 2012, 118: 399–406.

    [36] Patureau D, Zumstein E, Delgenes JP, et al. Aerobic denitrifiers isolated from diverse natural and managed ecosystems [J]. Microbial Ecology, 2000, 39(2): 145–152.

    [37] Zheng P. Environmental microbiology (2nd ed.) [M]. Hangzhou: Zhejiang University Press, 2012 (in Chinese).

    [38] Chèneby D, Philippot L, Hartmann A, et al. 16S rDNA analysis for characterization of denitrifying bacteria isolated from three agricultural soils [J]. FEMS Microbiology Ecology, 2000, 34(2): 121–128.

This Article

ISSN:0250-3301

CN: 11-1895/X

Vol 39, No. 12, Pages 5596-5604

December 2018

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion
  • 4 Conclusions
  • References