Distribution and Diversity of Ammonium-oxidizing Archaea and Ammonium-oxidizing Bacteria in Surface Sediments of Oujiang River

LI Hu1,2 HUANG Fu-yi1 SU Jian-qiang1 HONG You-wei1 YU Shen1

(1.Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China 361021)
(2.University of Chinese Academy of Sciences, Beijing, China 100049)

【Abstract】Ammonium-oxidizing archaea (AOA) and ammonium-oxidizing bacteria (AOB) play important roles in the biogeochemical nitrogen cycle. Rivers are important ecosystems containing a large number of functional microbes in nitrogen cycle. In this study, denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR (qPCR) technology were used to analyze the distribution and abundance of AOA and AOB in sediments from Oujiang River. The results showed that the AOA community structure was similar among various sites, while the AOB community structure was significantly different, in which all detected AOB sequences were classified into Nitrosospira and Nitrosomonas, and 90% affiliated to Nitrosospira. The community composition of AOA was influenced by NH4+ and TS; in addition, the AOB composition was affected by NH4+, EC, pH, NO3, TC and TN. Total sulfur (TS) and electrical conductivity ( EC) were the major factors influencing the diversity of AOA and AOB, respectively. AOA abundance was significantly higher than that of AOB. EC, NH4+-N and NO3-N were the main environmental factors affecting the abundance of AOA and AOB. This study indicated that the community composition and abundance of AOA and AOB were significantly influenced by environmental factors, and AOA might be the dominant driver in the ammonia oxidation process in Oujiang River surface sediments.

【Keywords】 ammonium-oxidizing archaea; ammonium-oxidizing bacteria; tidal reach; sediment; community composition; diversity;

【DOI】

【Funds】 Knowledge Innovation Project of Chinese Academy of Sciences (KZCX2-YWJC402) National Natural Science Foundation of China (31270153)

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(Translated by ZHEN Zhongzhi)

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This Article

ISSN:0250-3301

CN: 11-1895/X

Vol 36, No. 12, Pages 4659-4666

December 2015

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Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion
  • 4 Conclusion
  • References