Mechanism of Aerobic Exercise Intervention on Insulin Resistance in Rats Based on Pancreatic Metabonomics
(2.Modern Research Center of Chinese Medicine in Shanxi University, Taiyuan, Shanxi, China 030006)
【Abstract】Objective: This study aimed at exploring the mechanism of aerobic exercise intervention on insulin resistance (IR) by 1H-NMR metabolomics. Methods: Forty 2-month-old rats were randomly divided into normal diet group and high-fat diet group for 5 weeks. IR Rats were evaluated by hyperinsulinemic-glucose improved clamp and then divided into control group (N), model group (IR) and model exercise group (IRe). The rats of IRe group finished 6 weeks aerobics. After the experiment, all IR rats were examined by clamp, serum FINS was tested by FINS, pancreatic tissue metabolites were explored by 1H-NMR and analyzed by multivariate statistical analysis, metabolic pathways were detected by Metabo Analyst, and potential biomarkers were screened by the ROC. Results: 1) Compared with N group, FINS in IR group increased significantly and GIR decreased significantly. Compared with IR group, the body weight and FINS of IRe group decreased significantly and GIR increased significantly. 2) Compared with N group, 11 metabolites in the pancreas tissue of the IR group were different and were closely related to the six metabolic pathways, and 5 IR pancreatic biomarkers were found by ROC analysis. 3) Compared with IR group, 5 biomarkers, such as citric acid, glutamic acid and glutamine, were changed to different degrees in IRe group. Conclusions: This 6-week aerobic exercise can significantly improve the IR induced by high-fat diet, and its mechanism may be related the fact that aerobic exercise can alter the pancreatic biomarkers in different degrees in IR rats and then improve their metabolic pathways.
【Keywords】 aerobic exercise; insulin resistance; pancreas; metabonomics; metabolic pathway;
(Translated by PXJ1)
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