Analysis of Dynamic Changes in Production of Novel Coronavirus Antibodies Based on Three Kits

JIANG Zhongyi1 MAO Naiying2 ZHU Zhen2 ZHANG Yan2 CUI Aili2 WANG Peng1 XU Congshan1 DONG Maoxing1 WANG Xueying1 YU Deshan1 XU Wenbo2

(1.Gansu Provincial Center for Disease Control and Prevention, Lanzhou 730000)
(2.National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, NHC Key Laboratory of Medical Virology and Viral Diseases, Beijing 102206)

【Abstract】In order to understand the dynamic changes of virus-specific antibodies in the serum collected from coronavirus disease 19 (COVID-19) patients, three SARS-CoV-2 antibody detection kits with different principles were used in this study. Fifty-eight serum samples from 33 COVID-19 confirmed cases in Gansu Province, China were tested for virus-specific antibodies including IgM, IgG, and total antibodies. The detection results showed that the positive rates of IgM, IgG and total antibodies gradually increased with the time of onset: within the early 3 days, the positive rate of virus-specific antibody detected by three different kits ranged 13.6%–31.8%; within 4–7 days, the positive rate ranged 36.4%–45.5%; within 8–14 days, the positive rate ranged 55.6%–77.8%; after 15 days, the positive rate increased to 100%. In addition, three kits were used to detect serum samples collected from healthy people, and the specificity was 99%–100%. The statistical analysis indicated that there was no significant difference among the results of three kits (P > 0.05). In summary, three different principles of SARS-CoV-2 antibody detection kits have good sensitivity and specificity, which can provide assistant for clinical diagnosis; in addition, the dynamic characteristics of virus specific antibody from serum obtained through this study provide an important scientific basis for studies such as epidemiological investigation and recessive infection study.

【Keywords】 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Serology detection; Dynamic changes of antibody;

【DOI】

【Funds】 Major National Science and Technology Project (2018ZX10713-002, 2017ZX10103006)

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This Article

ISSN:1000-8721

CN: 11-1865/R

Vol 36, No. 03, Pages 343-347

May 2020

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Abstract

  • Materials and methods
  • Results
  • Discussion
  • References