Genetic Characteristics of Coxsakievirus A6 Circulating in Xinjiang, China, from 2012 to 2016

DENG Hong1 JI Tianjiao2 HUANG Yongdi1 HAN Taoli2 ZHANG Xuan1 SONG Xinxin1 ZHAO Jun1 AYIGULI·Yierhali1 WANG Fengying1 YUE Xihong1

(1.Xinjiang Center for Disease Control and Prevention, Urumqi, China 830002)
(2.National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China 102206)
【Novelty】It is the first time to analyze comprehensively and systematically the Genetic characteristics of coxsakievirus A6 circulated in Xinjiang of China.

【Abstract】To analyze the genetic characteristics of the VP1 region of coxsackievirus A6 (CV-A6) strains associated with hand, foot and mouth disease (HFMD) circulating in Xinjiang, China. A total of 75 strains of CV-A6 were isolated from the samples of HFMD patient in Xinjiang from 2012 to 2016. The nucleotide sequences of the VP1 region were amplified by reverse transcription polymerase chain reaction (RT-PCR) and sequenced. The homological analyses and phylogenetic tree of VP1 gene of CV-A6 were conducted using MEGA v5.05 and BioEdit v7.1.9 software. The 75 strains of CV-A6 shared nucleotide homology of 86.9%–100% with each other, and 81.4%–82.5% with the prototype strain (Gdula). The 71 strains in the D3 branch shared 93.1%–99.6% nucleotide homology with representative D3 strains. The 4 strains in the D2 branch shared 92.6%–96.5% nucleotide homology with representative D2 strains. According to the phylogenetic tree, all 75 strains of CV-A6 in Xinjiang belonged to D genotype, the majority of them (71/75) were in the D3 branch, belonged to the D3 subgenotype, with distribution in every year from 2012 to 2016. A few (4/75) in the D2 branch belonged to the D2 subgenotype. , and only separated in 2013 and 2014. The phylogenetic tree showed that Xinjiang CV-A6 strains formed several different small branches, and the strains in different prefectures or in different years were closely related to that in some regions in China, which suggested that the transmission chain and genotype distribution of Xinjiang CV-A6 was the same as that of the inland one and they evolved together. It is necessary to strengthen the pathogenic surveilliance, and related molecular characteristics analyses must be carried out.

【Keywords】 Hand, foot and mouth disease (HFMD) ; Coxsakievirus A6; Xinjiang Uygur Autonomous Region;


【Funds】 Natural Science Foundation of Xinjiang Uygur Autonomous Region (2016D01A063)

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This Article


CN: 11-1865/R

Vol 34, No. 06, Pages 839-843

November 2018


Article Outline



  • Materials and methods
  • Results
  • Discussion
  • References