Diagnosis of the First African Swine Fever in China
(2.School of Life Science and Engineering, Foshan University, Foshan, China 528231)
(3.Jilin Heyuan Bioengineering Limited by Share Ltd., Songyuan, China 138000)
【Abstract】A systematic diagnostic study was carried out according to the epidemic characteristics, clinical manifestations, anatomical changes, pathogenic morphology and molecular-biological characteristics of classical African swine fever (ASF), which occurred first in China. Epidemic characteristics were mainly pigs fed with food scraps and liquid waste, and were similar to those reported outside China. The clinical manifestations include fever, vomiting, skin cyanosis, constipation/diarrhea, stools with mucus/blood and other symptoms. Spleen enlargement, melanosis and fragility were the typical features of dissection, accompanied by hemorrhage in the heart, kidney, bladder, small intestine, mesenteric lymph nodes and inguinal lymph nodes. Electron microscopy revealed that the liver and spleen had virus-like particles of size 180–200 nm with multilayer envelopes. Polymerase chain reaction (PCR) and real-time PCR were reliable methods for a qualitative diagnosis. In conclusion, clinical manifestations are very important for the detection of African swine fever. Although the change of anatomy was not very specific, it could narrow the scope of examination and was of great importance in establishing a preliminary diagnosis (for which molecular-biology methods should be applied as soon as possible to complete the diagnosis).
【Keywords】 African swine fever (ASF) ; Suspected diagnosis; Deterministic diagnosis;
 Zhou X T, Li N, Luo Y Z, Liu Y, Miao F M, Chen T, Zhang S F, Cao P L, Li X D, Tian K G, Qiu H J, Hu R L. Emergence of African Swine Fever in China, 2018 [J]. Transbound Emerg Dis, 2018, epub ahead of print.
 Galindo I, Alonso C. African Swine Fever Virus: A Review [J]. Viruses, 2017, 9 (5): 103–113.
 African swine fever. In: Manual of diagnostic test and vaccines for terrestrial animals [S]. 2009: 1072–1076.
 Agüero M, Fernández J, Romero L J, Zamora M J, Sánchez C, Belák S, Arias M, Sánchez-Vizcaíno J M. A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples [J]. Vet Res, 2004, 35 (5): 551–563.
 Michaud V, Gil P, Kwiatek O, Prome S, Dixon L, Romero L, Le Potier M F, Arias M, Couacy-Hymann E, Roger F, Libeau G, Albina E. Long-term storage at tropical temperature of dried-blood filter papers for detection and genotyping of RNA and DNA viruses by direct PCR [J]. J Virol Methods, 2007, 146 (1): 257–265.
 Jiang Z J, Gong Z H, Yin Y B, Wang S S, Cai L J, Guo F S, Lu Z M, Sun C Y, Xuan H. Detection of African swine fever virus (ASFV) by polymerase chain reaction (PCR) [J]. Chinese Journal of Preventive Veterinary Medicine, 2000, 22 (S1): 114–117 (in Chinese with English abstract).
 Donald P K, Scott M R, Geoffrey H H, Sylvia S G, Philip J W, Linda K D, Armanda D S B, Trevor W D. Development of a TaqMan PCR assay with internal amplification control for the detection of African swine fever virus [J]. J Virol Methods, 2003, (107): 53–61.