Susceptibility of Influenza A (H3N2) Viruses to Neuraminidase Inhibitor Isolated during 2013–2017 in China's Mainland

HUANG Weijuan1 TAN Minju1 LI Xiyan1 CHENG Yanhui1 WEI Hejiang1 XIAO Ning1 ZHAO Xiang1 ZENG Xiaoxu1 WANG Dayan1

(1.Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health Commission, Beijing, China 102206)
【Novelty】The sensitivity of influenza A(H3N2) viruses to neuraminidase inhibitors that were prevalent in mainland China from 2013 to 2017 was first summarized in China, providing scientific basis for clinical use of antiviral drugs.

【Abstract】Antiviral-resistance data for 4 907 influenza A (H3N2) viruses isolated from 30 provinces (autonomous regions/municipalities) tested with aphenotypic method during 2013–2017 were analyzed to assess the susceptibility of influenza A (H3N2) viruses to neuraminidase inhibitors. All 4 907 influenza A (H3N2) viruses were sensitive to oseltamivir, although seven viruses (0.14%) exhibited reduced inhibition by zanamivir, among which six were detected in 2014. The IC50value tested was 24.03–49.21 folds greater than the reference IC50 value (0.28 nM). All six such viruses showed amino acid substitution of Q136K in the NA gene. In 2017, one virus showed mixed amino acid substitution of Q136Q/K in the NA gene, and the IC50 value tested was 10.90 folds greater than the reference IC50 value (0.31 nM). The seven viruses have been propagated in Madin-Darby canine kidney cells. Since the sequencing data for the original respiratory swab samples were not available, it cannot be excluded that the Q136K substitution arose in cell culture. These data indicated that circulating influenza A (H3N2) viruses in China's mainland form 2013 to 2017 retained susceptibility to neuraminidase inhibitors. This study provides a scientific basis for the use of clinical anti-influenza drugs.

【Keywords】 Influenza virus; A (H3N2) subtype; Neuraminidase inhibitor; Half-maximal inhibitory concentration; Susceptibility;

【DOI】

【Funds】 Emergency Prevention and Control Project of Ministry of Science and Technology (10600100000015001206)

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This Article

ISSN:1000-8721

CN: 11-1865/R

Vol 34, No. 06, Pages 793-799

November 2018

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Novelty

Abstract

  • Materials and methods
  • Results
  • Discussion
  • References