Supervisor(s): Central Station of Chinese Medicinal Materials Information Sponsor(s): Central Station of Chinese Medicinal Materials Information; State Food and Drug Administration CN：44-1286/R
Journal of Chinese Medicinal Materials is supervised by Central Station of Chinese Medicinal Materials Information and sponsored by Central Station of Chinese Medicinal Materials Information and State Food and Drug Administration. The journal covers research article of Chinese herbal medicine planting and raising technology, resource exploitation and utilization, concocted processing maintenance of medicinal herbs, identification, ingredient, pharmacology, preparations, and pharmacy.
The journal is included in CA, JST and CSCD.
Objective: To investigate the chemical constituents from the leaves of
Castanopsis eyrei. Methods: The extract from the leaves of
C. eyrei were isolated and purified by Sephadex LH-20, Toyopearl Butyl-650 C, Toyopearl HW-40F and other column chromatography, and their structures were identified on the basis of spectral data. Results: Thirteen compounds were isolated from the leaves of
C. eyrei and identified as crenatin (1), cretanin (2), chesnatin (3), 1,2,3,6-tetra-
O-galloyl-
β-
D-glucose (4), 1,2,3,4,6-penta-
O-galloyl-
β-
D-glucose (5), kaempferol (6), quercetin (7), myricetin (8), quercitrin (9), isoquercitrin (10), myricitrin (11), kaempferol-3-
O-
β-
D-glucoside (12), and kaempferol-3-
O-rutinoside (13), respectively. Conclusion: All the compounds are isolated from the plant for the first time.
Objective: To study the chemical constituents from the stems of
Gordonia kwangsiensis. Methods: The chemical constituents of 95% ethanol extract from the stems of
G. kwangsiensis were isolated and purified by various column chromatographic techniques, including silica gel, medium-pressure HPLC, RP-HPLC and preparative HPLC. Their structures were identified by MS,
1H-NMR,
13C-NMR and other spectroscopic analysis. Results: Nine compounds were isolated and purified from the stems of
G. kwangsiensis and identified as gordonoside I (1), gordonoside J (2), kaempferol-4′-
O-
α-
L-rhamnopyranosyl(1→6)-
β-
D-glucopyranoside (3), apigenin-7-
O-
β-
D-glucoside (4), 2
α,3
α,19
α-trihydroxyurs-12-en-28-
O-
β-
D-glucopyranoside (5), 2
α,3
β,19
α-trihydroxyolean-12-en-23,28-dioic acid (6),
β-daucosterol (7), betulinic acid (8), and oleanolic acid (9), respectively. Conclusion: All compounds are isolated from this plant for the first time.
Objective: To obtain the cellulose synthase-like protein(
Csl)gene in tuber of
Pseudostellaria heterophylla,to analyze the response of gene and
Pseudostellaria heterophylla polysaccharide to gibberellin treatment.Methods:Based on the homology of
Csl and the transcriptome database of
Pseudostellaria heterophylla,the
Csl gene sequence(
PhCslG) of
Pseudostellaria heterophylla was obtained by cloning.The tuber of
Pseudostellaria heterophylla treated with gibberellin and its inhibitor dotriazole with different concentrations was used as material,Real-time fluorescence quantitative PCR(qRT-PCR)and the polysaccharide content determination methods were used for quantitative analysis,the effects of gibberellin on the gene and polysaccharide content were analyzed.Results:The
PhCslG sequence had a open reading frame(2 205 bp),encoding 734 amino acids,the protein coded by the gene had two cellulose_synt conservative structure domain(pfam03552.14)of cellulose synthase gene encoding protein family and 2 D-D-D-QXXRW motifs.The results of qRT-PCR and polysaccharide content detection showed that the gibberellin with low or medium concentration promoted the up-regulation of
PhCslG expression,the content of polysaccharide increased,the effect of high concentration gibberellin was opposite.Conclusion:The
PhCslG gene sequence is cloned successfully for the first time,which provides a preliminary basis for further elucidation of the function of this gene and subsequent comprehensive research on the regulation mechanism of polysaccharide synthesis and accumulation of
Pseudostellaria heterophylla.
Objective: To understand the characteristics of the soil pH,organic matter,enzyme activity and effective components of
Bletilla striata tuber under different planting years.Methods:Taking
Bletilla striata growing soil and its tuber under different planting years as the research object,soil pH,organic matter,enzyme activity and medicinal components were detected and analyzed,and the relationship between soil pH,organic matter,enzyme activity and medicinal components was discussed.Results:The soil was neutral after planting for one year,weak acidic after planting for two year and weak alkaline after planting for three year;The variation rule of organic matter content with the planting years was planting for two year>planting for three year>planting for one year,soil polyphenol oxidase activity was planting for three year>planting for one year>planting for two year,soil phosphatase activity was planting for three year>planting for two year>planting for one year,soil urease and soil sucrase activities were planting for two year>planting for three year>planting for one year;The content of polysaccharides in
Bletilla striata was planting for three year>planting for two year>planting for one year,the content of total phenols was planting for two year>planting for one year>planting for three year.The relationship between soil pH and soil polyphenol oxidase,soil organic matter,soil sucrase was extremely significant positively correlated.The relationship between the total phenols and soil polyphenol oxidase,soil phosphatase,soil pH was significant negative correlated.The relationship between the total phenols and soil organic matter was significant positively correlated.Conclusion:The total phenols content of
Bletilla striata tuber is correlated with soil pH,soil enzyme activity and soil organic matter,but the effect is complicated.The content of polysaccharides in
Bletilla striata is positively correlated with planting years.
Objective: To explore the correlation between the section color and chemical composition contents of
Panax notoginseng. Methods: With
P. notoginseng from different producing areas as the research objects, HPLC was used to determine the contents of saponins in
P. notoginseng, and the chroma value of
P. notoginseng was obtained by color measuring instrument. The relationship between them was analyzed by SPSS 22.0 and GraphPad Prism 5. Results: There was highly significant negative correlation between the content of notoginsenoside R
1 and
a*, indicating that the smaller the
a*, the greener the powder color and the higher the content. Ginsenoside Rg
1, ginsenoside Re and five saponins content were significantly or highly significantly negatively correlated with
b*, indicating that the smaller the
b*, the bluer the powder color and the higher the contents. Ginsenoside Rb
1, ginsenoside Rg
1 and five saponins content were significantly negatively correlated with
L*, indicating that the smaller the
L*, the darker the powder color and the higher the contents. Ginsenoside Rd was not significantly correlated with
a*,
b* or
L*. The results of one-way ANOVA showed that except for notoginsenoside R
1, the contents of saponins in
P. notoginseng with gray-green section were mostly higher than that in the yellow-green section. Conclusion: Chroma value can be used to evaluate the quality of
P. notoginseng. This study can provide the basis for the identification of
P. notoginseng.
Objective: To investigate the expression level of miR-200b in the aortic tissues of diabetic rats and to explore the effect of Guanxin Tongluo Formula on vascular remodeling. Methods: Male SD rats conforming to foodborne obesity were selected. The diabetic rat model was established by single low dose intraperitoneal injection of 1% streptozotocin (STZ) at 28 mg/kg. These rats were randomly divided into the model group, Guanxin Tongluo Formula group (17.325 mg/kg) and atorvastatin calcium tablet (1.05 mg/kg) positive control group, continuously intervening for 12 weeks. A normal control group was set. Fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were measured at the end of the experiment. Carotid intima-media thickness (IMT), carotid peak systolic velocity (PSV), carotid end-diastolic flow velocity (EDV), vascular resistance index (RI), and pulsatility index (PI) were measured by carotid ultrasound. Hematoxylin-eosin (HE) and Victoria blue (VB) staining were used to observe the histopathological change and measure the thickness of aortic wall. The expression of mir-200b in aortic tissues was detected by real-time PCR. Immune protein imprinting method (Western blot) was used to detect transforming growth factor (TGF-
β1) and Collagen typeⅠ (CollagenⅠ) expression in aortic tissue. Results: Compared with the normal control group, the levels of FBG, TC, TG, LDL-C and RI in the model group were significantly increased, while HDL-C, PSV, EDV and PI levels were significantly decreased; IMT and the tube wall thickness increased significantly (
P < 0.05); the aortic endothelial injured; arterial elasticity fiber fractured; miR-200b expression level in aortic tissues was significantly down-regulated; TGF-
β1 and CollagenⅠ protein expression levels were significantly up-regulated (
P < 0.05). Compared with the model group, the levels of FBG, TC, TG, LDL-C and RI in the Guanxin Tongluo Formula group were significantly reduced, while HDL-C, PSV, EDV and PI levels were significantly increased. IMT and tube wall thickness were significantly decreased (
P < 0.05). Aortic endothelial injury and arterial elasticity fiber fracture conditions were significantly improved; miR-200b expression in aortic tissues was increased significantly; TGF-
β1 and CollagenⅠ protein expression levels were significantly decreased (
P < 0.05). Conclusion: MiR-200b in diabetic rat aorta tissues shows lower expression. Guanxin Tongluo Formula can reduce glucose, regulate lipid and inhibit or reverse vascular remodeling. Its mechanism may be related to the upregulation of miR-200b expression in aorta tissue sand the inhibition of TGF-
β1 and Collagen Ⅰ protein expression levels.
