Supervisor(s): Central Station of Chinese Medicinal Materials Information Sponsor(s): Central Station of Chinese Medicinal Materials Information; State Food and Drug Administration CN：44-1286/R
Journal of Chinese Medicinal Materials is supervised by Central Station of Chinese Medicinal Materials Information and sponsored by Central Station of Chinese Medicinal Materials Information and State Food and Drug Administration. The journal covers research article of Chinese herbal medicine planting and raising technology, resource exploitation and utilization, concocted processing maintenance of medicinal herbs, identification, ingredient, pharmacology, preparations, and pharmacy.
The journal is included in CA, JST and CSCD.
Objective: To study the chemical constituents of the ethyl acetate extract of the tubers of
Hemsleya pengxianensis var. jinfushanensis. Methods: The compounds were isolated by various chromatographic techniques, including silica gel, ODS reversed phase column and preparative high-performance liquid. The structures were identified on the basis of MS and NMR data. Results: Eleven compounds were obtained from the ethyl acetate fraction of the tubers of
Hemsleya pengxianensis var.
jinfushanensis and identified as scandenoside R8 (1), 3
-O-β-D-glucopyranosyl-3
β, 27-dihydroxycucurbita-5, 24 (
Z)-diene-11-one-27-
O-β-D-glucopyranoside (2), delavanoside A (3), scandenoside R3 (4),scandenoside R4 (5), jinfushanoside J (6), carnosiflosideⅤ(7), carnosifloside Ⅲ (8), scandenoside R6 (9), jinfushanoside K (10), amabiose (11). Conclusion: Compounds 4, 5, 7, 9, 11 are isolated from this plant for the first time, and the hydroxyl signals of compound 11 are identified for the first time.
Objective: To study the effects of different potassium levels on carbon-nitrogen metabolism and related enzymes of
Angelica sinensis, and to reveal the physiological basis of potassium on the quality of
A. sinensis, in order to provide theoretical support for the rational application of potassium fertilizer in cultivation practice of
A. sinensis. Methods: Different fertilization methods under field condition and different levels of K
2O (150 kg/hm
2, 300 kg/hm
2 and 450 kg/hm
2) were applied for evaluating the activities of related enzymes in carbon-nitrogen metabolism and related growth index, such as SPS, PK, SS, MDH, FUM, AGP, GAPDH, GS, GOGAT, NR and allantoinase, as well as the metabolisms of glucose, fructose, sucrose, total amino acids and total chlorophyll in the leaves of
A. sinensis. Results: Under two fertilizing methods as all basal application and 50% basal application + 50% topdressing, 300 kg/hm
2 potassium level was the optimum application rate, which significantly promoted the activities of SPS, PK, SS, MDH, FUM, AGP, GAPDH, GOGAT, allantion, and increased the contents of fructose, sucrose, total amino acids, total chlorophyll, while had the significant inhibitory effect on the activities of NR, GS in the leaves of
A. sinensis. The correlation analysis showed that the contents of glucose, fructose, sucrose, Chlorophyll a, Chlorophyll b, total chlorophyll and total amino acids had close positive correlation with root diameter (
P < 0.05). There was significant correlation between the contents of fructose, sucrose and total amino acids and underground dry weight (
P < 0.05), which indicated that the yield of
A. sinensis could be predicted by root diameter and underground dry weight. Conclusion: Proper potassium application rate can regulate the synthesis and accumulation of carbon-nitrogen metabolism products in the leaves of
A. sinensis, and its physiological basis is to improve the metabolic enzyme activity related in carbon-nitrogen metabolism products and the contents of related metabolism products.
Objective: To investage the possible mechanisms of Tanshinone Ⅱ
A promoting esophageal squamous cells (Eca109 cells) apoptosis. Methods: The human Eca109 cells were treated with Tanshinone Ⅱ
A for 48 h at different concentrations (2, 4, and 8 g/mL). The mRNA and protein expression levels of apoptosis related proteins BAX, Bcl-2, Caspase-3, Caspase-9, GRP78, XIAP were analyzed by real-time quantitative PCR (qPCR) and Western blotting. Results: Tanshinone Ⅱ
A inhibited the cell proliferation and induced apoptosis in Eca109 cells. The mRNA and protein expression levels of Bcl-2, XIAP, GRP78 were inhibited, while the mRNA and protein expression levels of BAX, Caspase-9 and Caspase-3 were increased, in a concentration-dependent manner. Conclusion: Tanshinone Ⅱ
A inhibits the growth of Eca109 cells and induces apoptosis possibly by suppressing cell apoptosis-related XIAP and GRP78 protein expression.
Objective: To set up a method for identifying and distinguishing Tibet
Cordyceps (TC) using X-ray diffraction (XRD). Methods: The power XRD technique were used to obtain the XRD patterns and their characteristic peaks for polypide and fungal stroma in one kind of TC and three kinds of inexpensive
Cordycepses (IC). Simulation of organic molecular crystals was applied to identify the constituent origin of XRD peaks of TC and IC. Results: The XRD spectra of IC consisted of a series of clear and discrete sharp peaks, which were confirmed to originate from
α-
D-mannitol and
δ-
D-mannitol, which was different from the artificial mannitol in crystalline phase; the content of mannitol in polypide of TC was three times that in fungal stroma. The XRD peaks of IC arose mainly from itaconic acid and a small amount of mannitol. The content of mannitol in IC was lower than 31% of that in TC. No mannitol existed in fungal stroma in all kinds of ICs and in polypide in one kind of IC. The link between constituents in TC and XRD, polymorphy phenomenon of mannitol, and quality control of TC, were discussed. Conclusion: XRD technique can provide an efficient means for rapidly and accurately identifying TC, and it has broad application prospects for identification and quality standard research of Chinese medicinal materials with higher-content organic molecular crystals.
Objective: To study the chemical constituents of
Mesona chinensis. Methods: The chemical constituents were isolated by chromatographic methods and structurally elucidated by spectral analysis. Results: Fifteen compounds were obtained and identified as rosmarinic acid (1), methyl rosmarinate (2), salviaflaside (3), salvianolic acid A (4), salviaflaside methyl ester (5), 9', 9'''-dimethyl lithospermate B (6), methyl lithospermate (7), lithospermic acid (8), lithospermic acid B (9), caffeic acid (10), 1-
O-[(
E)-caffeoyl]-
β-
D-glucopyranose (11), protocatechuic acid (12), danshensu (13), salvianolic acid J (14), 4-hydroxy-2, 6-dimethoxyphenol-1-
O-
β-
D-glucoside (15). Conclusion: Compounds 1–9, 11, 13–15 are isolated from Mesona genus for the first time, and compound 12 is isolated from this plant for the first time.
Objective: To evaluate the anti-influenza virus (H
3N
2) activity
in vitro of
Bletilla striata extracts, and to study the mechanisms. Methods: The viral hemagglutination titer and nucleic acid amount were used as measurement indicators to evaluate anti-influenza virus activity in embryonated chicken eggs from aqueous extracts and alcohol extracts of the
Bletilla striata. The cytopathic effect (CPE) and the hemagglutination inhibition (HI) assay were used to investigate the inhibitory effects and the targets of viral invasion. CPE and the levels of intracellular influenza RNA were carried out to evaluate the inhibition of viral replication and RNA synthesis. The neuraminidase inhibition (NAI) assay was employed to evaluate the effects of
Bletilla striata extracts on the neuraminidase activity (NA). Results: The study showed that the viral hemagglutination titer and nucleic acid amount reduced significantly (
P < 0.01) compared with the virus control after treated with the extracts of
Bletilla striata in embryonated chicken eggs. Both the aqueous extract and alcohol extract had good effect on virus inactivated directly, the highest inhibition rate reached (87.04 ± 7.81)% and (60.14 ± 6.88)% respectively. Aqueous extracts can block the viral attachment by inhibition of MDCK cells viral HA receptor. The aqueous extracts and ethanol extracts showed effective antiviral activity, compared with virus control,
Bletilla striata extracts can significantly reduce the flu virus RNA synthesis (
P < 0.01). Alcohol extracts had good neuraminidase inhibitory activities, NA-IC
50 was 16.0 mg/mL. Conclusion: The extracts of
Bletilla striata can play an anti-influenza virus effect by inhibiting virus HA receptor, and intervene the viral RNA synthesis and neuraminidase activity.
