Supervisor(s): Central Station of Chinese Medicinal Materials Information Sponsor(s): Central Station of Chinese Medicinal Materials Information; State Food and Drug Administration CN：44-1286/R
Journal of Chinese Medicinal Materials is supervised by Central Station of Chinese Medicinal Materials Information and sponsored by Central Station of Chinese Medicinal Materials Information and State Food and Drug Administration. The journal covers research article of Chinese herbal medicine planting and raising technology, resource exploitation and utilization, concocted processing maintenance of medicinal herbs, identification, ingredient, pharmacology, preparations, and pharmacy.
The journal is included in CA, JST and CSCD.
Objective: To select the best Ganoderma lucidum cultivation medium of replacing sawdust with loquat-branch dust, in order to realize high output and high quality production of Ganoderma lucidum. Methods: Loquat-branch dust was added as substitution in Ganoderma lucidum cultivation, and its effects on the biomass and the content of Ganoderma polysaccharides, triterpenoids and flavonoids were analyzed. Results: By using loquat-branch dust in culture, Ganoderma lucidum grew well with normal fruiting body obtained and spores released. Compared with control group, the biological efficiency was increased by 11.34%, when the addition of the loquat-branch dust was 80%, while the difference of the spore amount was little. When the addition of the loquat-branch dust was 90%, the content of Ganoderma polysaccharides and triterpenoids was increased by 32.29% and 30.58% respectively, while the influence on flavonoids was little. Conclusion: Using loquat-branch dust cultivation can improve the quality of Ganoderma lucidum. According to the comprehensive score, 80% loquat-branch dust is the most suitable cultivation medium.
Objective: To study the impact of climatic factors on the major medical component in Smilax china, and to supply a scientific and standard operation protocol on the introduction and cultivation. Methods: Detect the content of major medical components, such as baicalin and astibin, in Smilax china from eight counties of Xiangxi Autonomous Prefecture. And establish their relationship with annual average temperature, Jan average temp, Jul average temp, valid accumulative temp (≥ 10 °C), annual maximum temp, annual minimum temp, annual precipitation, annual sunshine amount, non-frost period and relative humidity by using the methods of partial least squares regression analysis (PLS). Results: Relative humidity, annual minimum temp and annual precipitation are the dominant factors. Annual minimum temp, annual average temp and valid accumulative temp were significantly correlated to the content of major medical components, thus, relative humidity, annual precipitation and non-frost period were negatively related to them. Conclusion: This study provides a scientific basis for resources protection, introduction and cultivation of Smilax china.
Objective: To establish the HPLC fingerprint of the whole plant of Nerviliae fordii and Nervilia plicata, in order to provide a method for evaluation of authenticity and quality control of the whole plant of Nerviliae fordii. Methods: 15 batches of Nerviliae fordii and 5 batches of Nervilia plicata were analyzed on a Kromasil C18 column (250 mm × 4.6 mm, 5 μm) at room temperature with gradient elution using acetonitrile and 0.2% phosphoric acid as the mobile phase at a flow rate of 1.0 mL/min. The wavelength of detector was 256 nm. Results: Significant differences were found between Nerviliae fordii and Nervilia plicata. Furthermore, there were no obvious differences observed between Nerviliae fordii of big-leaf and Nerviliae fordii of small-leaf. Conclusion: The present-developed HPLC fingerprints method provides a rapid, effective and valuable benchmark for distinguishing of Nerviliae fordii and Nervilia plicata, which is favorable to improve overall quality control of Nerviliae fordii.
Objective To study the chemical constituents of traditional Mongolian medicine Usnea longissima. Methods The compounds were isolated and purified by the methods of solvent extraction and chromatographic technique, and their structures were identified on the basis of the analyses of spectral data. Results Three compounds were obtained and identified as 4-hydroxy-2-[(2-hydroxy-4-methoxy-6-methylbenzoyl)oxy]-6- methylbenzoic acid (1), dibutyl phthalate (2) and diisobutyl phthalate (3). Conclusion Compound 1 is a new compound and named as isoevernic acid, compounds 2 and 3 are isolated from Usnea longissima for the first time.
Objective: To develop a procedure for preparing paclitaxel encapsulated PEGylated liposomes. Methods: The membrane hydration followed extraction method was used to prepare PEGylated liposomes. The process and formulation variables were optimized by “Box-Behnken Design (BBD)”of response surface methodology (RSM) with the amount of Soya phosphotidylcholine (SPC) and PEG 2000 -DSPE as well as the rate of SPC to drug as independent variables and encapsulation efficiency as dependent variables for optimization of formulation variables while temperature, pressure and cycle times as independent variables and particle size and polydispersion index as dependent variables for process variables. The optimized liposomal formulation was characterized for particle size, Zeta potential, morphology and in vitro drug release. Results: For encapsulation efficiency, particle size, polydispersion index, Zeta potential, and in vitro drug release of PEGylated liposomes was found to be 80.3%, (97.15 ± 14.9) nm, 0.117 ± 0.019, (−30.3 ± 3.7) mV, and 37.4% in 24 h, respectively. The liposomes were found to be small, unilamellar and spherical with smooth surface as seen in transmission electron microscopy. Conclusion: The Box-Behnken response surface methodology facilitates the formulation and optimization of paclitaxel PEGylated liposomes.
Objective: To identify the metabolites of higenamine, liensinine, isoliensinine and neferine in Caco-2 cells by LC/MS/MS. Methods: After Caco-2 cells were treated with higenamine, liensinine, isoliensinine or neferine for 3, 6 and 12 h, samples were collected, purified and then analyzed by LC/MS/MS. The structures of the metabolites were elucidated by molecular masses, retention times, MS and MS/MS spectra comparing with those of the parent drug. Results: The procedure identified that the major metabolites of higenamine were methyl-higenamine and higenamine-glucuronide, the major metabolite of liensinine was demethyl-liensinine, the major metabolite of isoliensinine was demethyl-isoliensinine, the major metabolites of neferine were liensinine, isoliensinine and their further demethylation products. Conclusion: LC/MS/MS is simple, rapid and sensitive for the metabolites identification. Methylation, demethylation and glucuronidation are main metabolic pathways of alkaloids from Nelumbinis Plumula in Caco-2 cells.
Objective: To investigate the anti-aging effects of Urtica polysaccharide on subacute aging mice induced by D-galactose. Methods: A total of 90 C57BL mice were randomly divided into the following six groups: a normal control group, a model group, a VE (100 mg/kg) positive control group, a high-dose (200 mg/kg) Urtica polysaccharide group, a medium-dose (100 mg/kg) Urtica polysaccharide group and a low-dose (50 mg/kg) Urtica polysaccharide group. The normal control group was subcutaneously injected with normal saline (10 mL/kg) daily, while mice in other groups were subcutaneously injected with 150 mg/kg D-galactose for modeling. Mice in six groups were intragastrically administrated with corresponding drugs, while those in the normal control and modeling groups were intragastrically administrated with the same volume of normal saline. After administration for 42 days, the mice weighted were subjected to the eight-arm maze test and swimming endurance test. Then, the mice were euthanized and their serum, liver and brain tissues were collected for determination of total protein content. Meanwhile, the total anti-oxidative capacity (T-AOC) in the liver and brain, the activities of total superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) content were measured using corresponding kits. Results: Compared with the model group, mice exposed to Urtica polysaccharide showed more body weight gain and improved swimming endurance, required shorter time to cross the maze, produced less time of errors but remarkable increases in the protein content within the serum, liver and brain tissues, T-AOC in the liver and brain, and SOD and GSH-Px activities, in addition to significant decreases in MDA content in the liver and brain. Conclusion: Urtica polysaccharide has anti-aging effects on D-galactose induced aging mice, which may be associated with antioxidation.
Abstract Objective: This study is aimed to screen and verify the effective fractions of
Aegiceras corniculatum (L.) Blanco leaves for the anti-inflammatory and analgesic activities. Methods:
Aegiceras corniculatum (L.) Blanco leaves were extracted by 75% ethanol to obtain five fractions, petroleum ether fraction, dichloromethane fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction. Acetic acid-induced writhing test in mice screened out ethyl acetate fraction with stronger anti-inflammatory and analgesic effects, which were then comprehensively evaluated through a series of tests in mice such as acetic acid-induced writhing test, the hot-plate test, carrageenan-induced paw edema and xylene-induced ear edema. Spectrophotometer was used to detect the total superoxide dismutase (T-SOD) activity and malondialdehyde (MDA) content in liver homogenate of mice with carrageenan-induced paw edema. Results: After i.g. administration of high dose (200 mg/kg) ethyl acetate fraction of
Aegiceras corniculatum (L.) Blanco leaves, acetic acid-induced writhing times of mice were significantly reduced, pain threshold of mice in the hot-plate test increased, and the degrees of carrageenan-induced paw edema and xylene-induced ear edema were inhibited (
P < 0.01). Three days after i.g. administration of ethyl acetate fraction of
Aegiceras corniculatum (L.) Blanco leaves of different doses (100, 150 and 200 mg/kg), T-SOD activity was significantly elevated and MDA content significantly decreased in the liver of mice with carrageenan-induced paw edema (both
P < 0.01). Conclusion: Ethyl acetate fraction of
Aegiceras corniculatum (L.) Blanco leaves has significant anti-inflammatory and analgesic effects and antioxidant effect may be one of the mechanisms underlying the anti-inflammatory effect.
