Supervisor(s): Central Station of Chinese Medicinal Materials Information Sponsor(s): Central Station of Chinese Medicinal Materials Information; State Food and Drug Administration CN：44-1286/R
Journal of Chinese Medicinal Materials is supervised by Central Station of Chinese Medicinal Materials Information and sponsored by Central Station of Chinese Medicinal Materials Information and State Food and Drug Administration. The journal covers research article of Chinese herbal medicine planting and raising technology, resource exploitation and utilization, concocted processing maintenance of medicinal herbs, identification, ingredient, pharmacology, preparations, and pharmacy.
The journal is included in CA, JST and CSCD.
Objective: The chemical differences of Polygala tenuifolia varieties-JinYuan 1(JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia. Methods: The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis. The obtained data were analyzed using Principal Component Analysis (PCA) and other statistical analysis methods, and differential metabolites were further figured out. Results: Compared with FY, sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot. Compared with JY1. The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides (such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot. In addition, the relative contents of sibiricaxanthone A, 3, 6'-disinapoly sucrose and senegin Ⅲ showed significant differences among FY, JY1 and FY2. Conclusion: As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY, which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
Objective: To establish an HPLC-UV method for determining pharmacokinetic difference of notoginsenoside R1 between normal rats and ischemic rats. Methods: 48 male SD rats were randomly divided into normal group and acute myocardial ischemia (AMI) model group induced by pituitrin and each group was classified into high, middle and low-dose of groups with notoginsenoside R1 (200, 100 and 50 mg/kg) respectively. Blood samples were collected at different points in time after they were administered once by gavage and separated by Waters symmetry C18 column (250 mm × 4.6 mm, 5 μm) under the detective wavelength 203 nm, the mobile phase was acetonitrile-water with icariin as the internal standard and the pharmacokinetic parameters were calculated by DAS 2.0. Results: Notoginsenoside R1 had good linearity in the ranges of 0.2 to 125 μg/mL (R2 = 0.9997) with SNR 1:3 and the lowest detection limit was 0.053 μg/mL, the extraction rate, RSDs of within-day and between-day, specificity, accuracy and precision accorded with the requirement of bio-sample pretreatment. Compared to the normal group, AUC0-t and AUC0-∞ was significantly increased (P < 0.01) and the terminal half-life was prolonged markedly (P < 0.01) in AMI group. Conclusions: The method is simple, accurate and had high specificity and sensitivity, that could be applied in quantitative determination of notoginsenoside R1 and research of pharmacokinetics; the relative bioavailability of notoginsenoside R1 is increased significantly in AMI group, which indicates that notoginsenoside R1 has better effect in model rat.
Objective: To investigate the immunomodulatory effects of polysaccharide from Hedyotis diffusa on immunosuppressive mice. Methods: Eighty healthy Kunming mice were randomly divided into normal control group (intragastric administration of normal saline), immunosuppressive model group (intragastric administration of normal saline) and three medication groups, including low, middle and high dose groups of polysaccharide from Hedyotis diffusa (50, 100, and 200 mg/kg, respectively, intragastric administration). Except the normal control group, model group and medication groups were given cyclophosphamide (CTX, 100 mg/kg) for consecutively 3 d by intraperitoneal injection to induce immunosuppressive model. After modeling, each group was given corresponding drugs by intragastric administration, once every hour. After consecutively 15 d, immune parameters, including coefficients of immune organs, serum hemolysin, splenic lymphocyte proliferation, NK cell activity in the spleen and content of serum cytokine (IL-2, IL-6 and TNF-α) in mice, were detected. Results: Compared with the normal control group, coefficients of spleen and thymus, serum hemolysin, splenic lymphocyte proliferation, NK cell activity in the spleen and content of serum IL-2, IL-6 and TNF-α significantly decreased in the model group (P < 0.05 or P < 0.01). Compared with the model group, middle and high dose of polysaccharide from Hedyotis diffusa can significantly improve the coefficients of immune organs of mice and serum hemolysin (P < 0.05 or P < 0.01) and significantly promote LPS-induced splenic lymphocyte proliferation and NK cell activity (P < 0.01); ConA-induced splenic lymphocyte transformation was significantly promoted (P < 0.05 or P < 0.01) and the content of serum IL-2, IL-6 and TNF-α was significantly elevated (P < 0.01) in each medication group. Conclusion: Polysaccharide from Hedyotis diffusa can improve the immunological functions of immunosuppressive mice.
Objective: To establish a novel, accurate and valid fingerprint method of Zhenyuan granules dry extract by using HPLC-DAD method, to study herbs belonging of fingerprint peaks and to identify some of the chromatographic peaks by HPLC-MS/MS analysis, for providing the basis for scientific evaluation of the quality. Methods: The sample solutions were analyzed by an Agilent SB C18 (250 mm × 4.6 mm, 5 μm) column, and gradiently eluted with acetonitrile (containing 0.1% formic acid) and aqueous phase (containing 0.1% formic acid) as the mobile phase. The flow rates were 1.2 mL/min (0–70 min) and 0.8 mL/min (70–150 min); the column temperature was 30 °C; and the detection wavelength was 254 nm. Results: 40 peaks were selected as fingerprint peaks under the optimal chromatographic condition, and the similarity coefficients of 10 batches of Zhenyuan granules dry extract were all greater than 0.98. Totally 27 peaks were tentatively identified with reference to literature data based on their mass spectrometry. Conclusion: The chromatographic fingerprint of Zhenyuan granules is proved to be a reliable method for comprehensive quality control and assessment.
Objective: Based on backtracking method, identification and quality evaluation of Xiaoyin Jiedu Decoction (XYJDD) by HPLC fingerprint analysis were carried out. Methods: HPLC-DAD fingerprint of XYJDD was conducted with Dikma Platisil ODS C18 column (250 mm × 4. 6 mm, 5 μm) and gradient elution with the mobile phase consisting of acetonitrile–(0.1% phosphoric acid–0. 1% triethylamine solution) at column temperature of 30 °C. On the basis of the established chromatographic pattern of XYJDD, tracking backward to the corresponding crude herbal drugs in the formula and the attribution of most peaks in the XYJDD fingerprint can be figured out. Results: 16 peaks of XYJDD HPLC fingerprint were assigned by parallel comparison with the fingerprint of the five corresponding crude drugs in the formula of Scutellariae Radix, Glycyrrhizae Radix et Rhizoma, Houttuyniae Herba, Aurantii Fructus, Arnebiae Radix. Four peaks can be identified with the chemical reference substances. Conclusion: The entirety of XYJDD HPLC fingerprint enhances the specialty for control and assessment of the product quality, and the backtracking experimental method can be expected to be applied in chromatographic fingerprinting analysis of complex Chinese patent medicine.
Objective: To provide evidences for identification of Gentianae Macrophyllae Radix by comparing the morphological characteristics of six species of Sect. Cruciata (Gentiana macrophylla, G. crassicaulis, G. straminea, G. dahurica, G. officinalis and G. siphonantha). Methods: By microscope, the tissue characteristics with freehand section of the upper, middle and lower of root and the powder characteristics with chloral hydrate were studied. Results: The vascular cylinder of G. crassicaulis was not split. The vascular cylinder of G. macrophylla, G. dahurica and G. officinalis, were only split in the upper part. The roots of G. straminea and G. siphonantha were completely divided into several smaller roots twisting together. There were a lot of sclerenchyma cells in the powder of G. dahurica, G. straminea and G. siphonantha, but their shapes were different. No sclerenchyma cells were found in the other three species. Conclusion: There are obviously differences among the microscopic morphological characteristics of root of six species of Sect. Cruciata, which can provide the basis for identification of Gentianae macrophyllae Radix.
