Supervisor(s): Central Station of Chinese Medicinal Materials Information Sponsor(s): Central Station of Chinese Medicinal Materials Information; State Food and Drug Administration CN：44-1286/R
Journal of Chinese Medicinal Materials is supervised by Central Station of Chinese Medicinal Materials Information and sponsored by Central Station of Chinese Medicinal Materials Information and State Food and Drug Administration. The journal covers research article of Chinese herbal medicine planting and raising technology, resource exploitation and utilization, concocted processing maintenance of medicinal herbs, identification, ingredient, pharmacology, preparations, and pharmacy.
The journal is included in CA, JST and CSCD.
Objective: To confirm the current major diseases and corresponding pathogens of Codonopsis tangshen in Chongqing. Methods: The main cultivation regions of Codonopsis tangshen in Chongqing were systematically investigated, and the pathogens of the obtained specimens were isolated and identified. Results: Totally, five fungal diseases in Codonopsis tangshen were identified, including rust disease (Puccinia campanumoeae Pat.), root rot (Fusarium oxysporum Schl.), violet root rot (Helicobasidium mompa Tanaka), powdery mildew (Sphaerotheca codonopsis (Golov.) Z. Y. Zhao), and blight (Septoria codonopsidis Ziling). Currently, the diseases with the serious damage on Codonopsis tangshen included rust disease, root rot and violet root rot. Conclusion: Rust disease, the severest disease, whose incidence reaches 100%, is an urgent problem waiting to be solved effectively in Codonopsis tangshen cultivation.
Objective: To explore the regulation effects of oxygen carriers on Poria cocos submerged fermentation system which usually can be seriously inhibited by dissolved oxygen limitation. Methods: One-factor-at-a-time design was employed to determine the oxygen carrier addition strategy through analyzing the effects of different oxygen carries, concentration and adding time of oxygen carrier on Poria cocos submerged fermentation. Then the oxygen carrier addition strategy was established and the metabolic processes of Poria cocos submerged fermentation were investigated comprehensively. Results: The optimal oxygen carrier addition strategy was adding 1% (V/V) Tween-80 at 48 h after inoculation. Under this optimized condition, dry cell weight of Poria cocos reached 13.43 g/L in a 10 L bioreactor, while yields of exopolysaccharides and pachymic acid were 8.58 g/L and 989.52 μg /L, respectively, which exhibited obvious promoting effects compared with no addition oxygen carrier fermentation process. Conclusion: Tween-80 can remarkably increase the levels of cell growth, exopolysaccharides biosynthesis and pachymic acid in Poria cocos submerged fermentation system, which may provide new reference for further exploring dissolved oxygen limitation in high density fermentation of medical fungi efficiently.
Objective: To improve the traditional fingerprint method to distinguish vinegar processed Genkwa Flos from raw Genkwa Flos. Methods: Ten batches of vinegar processed Genkwa Flos were collected, processed with vinegar through a standard method, and then analyzed under the optimum HPLC condition. Based on the chromatographic data obtained, a common model of vinegar processed Genkwa Flos fingerprints, including 11 common peaks was established and the components genkwanin, hydroxygenkwanin, luteolin, apigenin and yuanhuacin were identified,. The peak of baicalein, an exogenous component added quantitatively to the samples as an internal standard, served as the reference peak. The similarity between the test samples and the common model was computed using the improving Euclidean distance method developed in this paper. Results: The similarities between vinegar processed Genkwa Flos samples and the common model were higher than 0.9, whereas those between raw Genkwa Flos and the common model were lower than 0.9. Conclusion: The proposed method thus effectively provides a clear distinction between vinegar processed and raw Genkwa Flos samples. The result is helpful to ensure the safe clinical use of the plant and expand the application field of fingerprinting technology.
Objective: To study the genuineness recognition of traditional Chinese medicine by analyzing fingerprint similarity using information entropy theory, Aurantii Fructus was used as the model drug. Methods: Fingerprints of different sources of Aurantii Fructus were analyzed by HPLC. “Jiang Aurantii Fructus” genuineness was evaluated by analyzing fingerprint similarity using information entropy theory and compared with the results of traditional calculated methods. Results: The new method obviously improved the effect of genuineness recognition of “Jiang Aurantii Fructus”. Conclusion: This method provides scientific reference for “Jiang Aurantii Fructus” genuineness identification.
Objective: To study the chemical constituents of the root of Salvia przewalskii. Methods: The chemical constituents were isolated and purified by means of chromatographic techniques and their structures were elucidated by spectroscopic methods. Results: 17 compounds were isolated and identified as danshenxinkun B (1), danshenol C (2), isocryptotanshinone (3), 1, 2-dihydroisodihydrotanshinone-I (4), isotanshinone-IIA (5), dihydrotanshinone-I (6), tanshinlactone (7), danshenol B (8), tanshinone-I (9), danshenspiroketallactone (10), ferruginol (11), epi-danshenspiroketallactone (12), danshenxinkun A (13), sapriolactone (14), 12-hydroxy-6, 7-secoabieta-8, 11, 13-trien-6, 7-diol (15), 6α-hydroxysugiol (16) and hypargenin B (17). Conclusion: Compounds 1–8 are isolated from this plant for the first time.
Objective: To study the chemical constituents in the stem bark of Styrax perkinsiae. Methods: The chemical constituents were separated and purified by chromatographic methods after solvent extraction and identified by spectroscopic analyses. Results: Ten lignans were isolated from the stem bark of Styrax perkinsiae and identified as following: pinoresinol 4-O-β-D-glucopyranoside (1), matairesinoside (2), styraxlignolide B (3), 3-(β-D-glucopyranosyloxymethyl)-2-(4-hydroxy-3-methoxyphenyl)-5-(3-hydroxypropyl)-7-methoxy-(2R, 3S)-dihydrobenzofuran (4), burselignan (5), (+)-neo-olivil (6), threo-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1, 3-propanediol (7), erythro-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1, 3-propanediol (8), isolariciresinol (9) and (+)-lariciresinol (10). Conclusion: Compounds 5–10 are isolated from the plants of genus Styrax for the first time.
Objective: To investigate the steroidal glycoside constituents of Solanum cumingii. Methods: The compounds were isolated by silica gel, Sephadex LH-20, RP-C18 column and Pre-HPLC chromatography. Their structures were identified by ESI-MS and NMR. Results: Six known compounds including torvoside K (1), torvoside J (2), torvoside L (3), khasianine (4), aculeatiside A (5) and solamargine (6) were isolated from S. cumingii. Conclusion: All compounds are isolated from S. cumingii for the first time.
Objective: HPLC-UV-ELSD was established to simultaneously determine the content of nine constituents in Lonicera macranthoides Hand. (neochlorogenic acid, cryptochlorogenic acid, galuteolin, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid, 4, 5-dicaffeoylquinic acid, macranthoidin B and dipsacoside B). Method: A Shiseido C18 (250 mm × 4.6 mm, 5 μm) chromatographic column was used with 0.2% formic acid (A)-acetonitrile solution (B) as the mobile phase by gradient elution. The flow rate was 1.0 mL/min, the column temperature was 35 °C, and the detection wavelength was set at 326 and 350 nm. The detection temperature of ELSD drift tube was 100 °C. And the flow rate of carrier gas (nitrogen) was set at 3 L/min. Results: Within the observatin range, injection volume and the corresponding peak area showed a good linear relation (r > 0.998 3), precision RSD < 2.5%, repeatability RSD < 3.8%, and average recovery was in the range of 96.3%–100.7%. Conclusion: The established method was accurate, rapid and of good repeatability; it can be used to determine the content of nine constituents in Lonicera macranthoides Hand.
Objective: To investigate the protective effects of total saponins of P. japonicus (TSPJ) on H2O2-induced oxidative stress damage in SH-SY5Y cells, and to explore the underlying mechanism. Methods: H2O2-treated SH-SY5Y cells was used to build up oxidative stress damage model. SH-SY5Y cells were incubated with 600 μmol/L H2O2 for 12 h, then treated with various concentrations of TSPJ (0.1, 1, 5 and 20 μg/mL) for 12 h, and then incubated with 600 μmol/L H2O2 for 12 h. Cell viability was detected by MTT assay. Superoxide dicmutase (SOD) activities and malondialdehyde (MDA) contents were measured by biochemical assay kits. Protein levels of Nrf2, p-ERK, and p-P38 were detected by Western blotting. Levels of NQO1 and GCLC mRNA expression were determined by real-time PCR. Results: Compared with control group, H2O2 stimulated the decrease of cell viability and SOD activities as well as the increase of MDA contents, which were reversed by TSPJ treatment. Furthermore, TSPJ treatment up-regulated not only the decreased protein expressions of Nrf2 and p-ERK but also the decreased mRNA expression of NQO1 and GCLC. Conclusion: TSPJ can protect SH-SY5Y cells from H2O2-induced oxidative stress damage. The mechanism may be related to up-regulating the phosphorylation of ERK thereby promoting the Nrf2 nuclear translocation and increasing the mRNA expression of antioxidant genes such as NQO1 and GCLC.
Objective: To explore the cardioprotective effect and its mechanism of total saponins of Panacis Majoris Rhizoma in myocardial infarction (MI) rats. Methods: The MI model rats induced by ligating anterior descending branch of coronary artery were randomly divided into four group: model group, total saponins of Panacis Majoris Rhizoma (100 and 200 mg/kg) groups and compound Danshen dripping pills group. The rats were orally administrated with drugs once a day for four weeks. Another rats were selected as sham operation group. After four weeks intervention, cardiac function was examined, the serum levels of TNF-α, IL-1β, IL-6 and IL-8 were measured by using ELISA, respectively. The myocardial hypertrophy index was investigated, the myocardial infarct size, degree of ventricular dilatation, myocardial interstitial collagen volume fraction and tissue morphology were investigated by HE, Masson, picric acid-sirius red staining and observing with alight microscope and electron microscope. Protein expressions of phosphorylation IκB-α (pIκB-α) and NF-κBp65 in heart tissue were detected by Western blotting. Results: Total saponins of Panacis Majoris Rhizoma might significantly decrease the levels of serum TNF-α, IL-1β, IL-6 and IL-8; decrease myocardial hypertrophy indexes, myocardial infarct size, degree of ventricular dilatation and myocardial interstitial collagen volume fraction; improve heart tissue morphology and cardiac function; downregulate protein expression of pIκB-α and NF-κBp65; and upregulate protein expression of SIRT1. The aforementioned action effects of total saponins of Panacis Majoris Rhizoma (200 mg/kg) were similar with compound Danshen dripping pills. Conclusion: Total saponins of Panacis Majoris Rhizoma possesses cardioprotective effect against ligating left anterior descending branch induced MI in rats. The mechanism may be related to strengthening SIRT1 expression, inhibiting the phosphorylation of IκB-α, and finally inhibiting the activation of NF-κB and proinflammatory production.
