Supervisor(s): Central Station of Chinese Medicinal Materials Information Sponsor(s): Central Station of Chinese Medicinal Materials Information; State Food and Drug Administration CN：44-1286/R
Journal of Chinese Medicinal Materials is supervised by Central Station of Chinese Medicinal Materials Information and sponsored by Central Station of Chinese Medicinal Materials Information and State Food and Drug Administration. The journal covers research article of Chinese herbal medicine planting and raising technology, resource exploitation and utilization, concocted processing maintenance of medicinal herbs, identification, ingredient, pharmacology, preparations, and pharmacy.
The journal is included in CA, JST and CSCD.
Objective: To provide technical support for industrialization promotion of tetraploid of Dioscorea zingiberensis,the manufacturing method for synthetic seeds of tetraploid of Dioscorea zingiberensis was established and the correlated influential factors were studied. Methods: By taking embryogenic calluses of tetraploid of Dioscorea zingiberensis as propagation materials,the influential factors such as components of artificial endosperm,seed coats,storage conditions and germination materials on germination and seedling of the synthetic seeds were evaluated. Results: When 4% alginate + 2% Ca Cl2+ 2% chitosan was served as seed coat materials,and 1 /2 MS+ 0.2 mg/L BA + 0.5 mg/L NAA + 0.1 mg/L penicillin + 0. 3% carbendazim powder + 0.2% sodium benzoate + 1.0% sucrose +0.5% activated carbon + 1.0% tapioca starch was served as endosperm,the synthetic seeds had high germination rate and seedling rate. After storing at 4 oC for 20 d, the germination rate and seedling rate of synthetic seeds was 76.7% and 71.7%, respectively. Conclusion: Manufacturing technology of synthetic seeds of tetraploid of Dioscorea zingiberensis with embryogenic calluses as propagation materials has production prospects.
Objective: To study the effects of aphid occurring on the yield and quality of Lonicera macranthoides. Methods: The yields of fresh and dry flowers in the same plant with different aphid damage were caculated. Content of chlorogenic acid, caffeic acid, luteolin-7-O-glucoside, isochlorogenic acid A, macranthoidin B and dipsacoside B in Lonicera macranthoides which was damaged by aphid were determined by HPLC-ELSD. Results: Aphid accurrence significantly affected the yield of the Lonicera macranthoides, and extremely significantly affected content of luteolin-7-O-glucoside, macranthoidin B and dipsacoside B (P < 0. 01), and significantly affected the content of chlorogenic acid (P < 0. 05), but had no obvious effect on the content of caffeic acid and isochlorogenic acid A in the Lonicera macranthoides. Conclusions: It was supposed to regulate prevention and control of aphids in Lonicera macranthoides GAP production.
Objective: To establish a simple, rapid and efficient method for determination of different inorganic elements in Euryale Semen from different habitats. Methods: Inductively coupled plasma-optical emission spectrometry (ICP-OES) was applied to determine inorganic elements in Euryale Semen, and the results were analyzed by principal component analysis. Results: Euryale Semen from different habitats contained the kind of inorganic elements ranging from 22 to 26, including micronutrient elements like Iron, Zinc, Selenium, Copper, Molybdenum, Chrome and Cobalt, as well as macronutrient elements such as Potassium, Calcium, Sodium, Magnesium and Phosphorus. Five factors were extracted and used to comprehensively evaluate Euryale Semen from 20 different habitats covered almost China. The comprehensive function was F = 0.38828F1 + 0.25603F2 + 0.07617F3 + 0.06860F4 + 0.04868F5, which resulted in the top three samples coming from Jiangsu Gaoyou, Hunan Xiangxi and Jiangsu Suzhou respectively. Conclusion: The study indicates that ICPOES is a quick, accurate and sensitive method to determine the contents of inorganic elements in Euryale Semen,which provides scientific and reliable reference for its quality control and safety assessment.
Objective: To quantify the amount of tetramethylpyrazine in Szechwan Lovage Rhizome (Chuanxiong, the rhizome of Ligusticum chuanxiong Hort.,CX) and Cnidium Rhizome (Japanese Chuanxiong, the rhizome of Cnidium officinale Makino, JCX) for quality assessment. Methods: An HPLC-DAD-MS technique was employed to detect tetramethylpyrazine in 27 CX and 10 JCX samples. Tetramethylpyrazine was separated on a Waters Symmetry C18column (250 mm × 4.6 mm, 5 μm). The mobile phase was methanolacetonitrile-water (27:1:72) at a flow rate of 1.0 mL/min. The column temperature was 35 °C. DAD detection wavelength was 280 nm, while electrospray ionization detector was set at positive mode to collect MS spectrum. Results: In the total of 37 herb samples, 11 samples were found to contain tetramethylpyrazine with the mean amount of 2.19 μg/g (n = 11). Six of 27 CX samples and 5 of 10 JCX samples were found the existence of tetramethylpyrazine with the amount of 0.60–11.75 μg/g and 0.61– 3.05 μg/g, respectively. The correlation was not found between tetramethylpyrazine and the cultivation area, morphological character, processing or storage method for CX and JCX samples. It was possible that tetramethylpyrazine resulted from the microbes in soil. Conclusion: The developed method is accurate to quantify tetramethylpyrazine in CX and JCX herbs. Both the two herbs indeed contain tetramethylpyrazine, but it is not suitable to be a chemical marker to assess the quality of CX and JCX owing to low content.
Objective: To study the chemical constituents from Kalimeris indica. Methods: The chemical structures were identified by pob data which can be obtained by many methods of silica gel, Sephadex LH-20 and preparing HPLC, etc. Results: Fourteen compounds were isolated and identified as α-spinasterol(1), dibutylphthalate(2), (22 E, 24R)-5α, 8α-epidioxy-ergosta-6, 22-dien-3β-ol(3), oleanolic acid(4), coniferyl alcohol(5), umbelliferone(6), syringaresinol(7), 15-oxo-14, 16H-strictic acid(8), lariciresinol(9), (Z)-3, 7, 11-trimethyl-1, 6-dodecadien-3, 10, 11-triol(10), neoechinulin A(11), pinoresinol(12), 4-allyl-3, 5-dimethoxyphenol(13), and 3, 4, 5-trimethoxyacetophenone(14). Conclusion: Except compound 1, the other compounds are isolated from this platymiscium for the first time.
Objective: To study the chemical constituents from the hypocotyls of mangrove Bruguiera gymnorrhiza. Methods: The chemical constituents were isolated and purified by recrystallization, silica gel column chromatography and semi-preparative HPLC. Their structures were identified by spectroscopic analysis and comparison with literatures. Results: Seven compounds were isolated and their structures were identified as 3-β-(Z)-coumaroyllupeol (1), dioslupecin(2), cholesterol (3), menisdaurillide(4), aquilegiolide (5), vomifoliol (6) and roseosideⅡ (7). Conclusion: Compounds 1, 2 and 4–7 are isolated from this plant for the first time.
Objective: To investigate the chemical constituents with anti-hypoxia activity from Saussurea involucrata. Methods: The chemical constituents, isolated and purified by column chromatography from Saussurea involucrata,were identified by several spectroscopic methods. The anti-hypoxic activities of these compounds were examined using the normobaric hypoxic model of mice. Results: Twelve compounds were isolated from petroleum ether extract of Saussurea involucrata and identified as n-octacosane (1),1-undecanol (2), heptadecan-1-ol (3), heptacosan-1-ol (4), myristicin (5), apiol (6), β-sitosterol (7), lupeol (8), moslosooflavone (9), mosloflavone (10), negletein (11), and 5, 6-dihydroxy-7, 8-dimethoxyflavone(12). Conclusion: All compounds except 7 and 8 were isolated from this plant for the first time. Compound 1, 5 and 8–12 can significantly prolong the survival time of hypoxic mice.
Objective: To investigate the chemical constituents of Rubus alceaefolius. Methods: Nine compounds were isolated and purified from the petroleum ether extract of 95% alcohol extract of Rubus alceaefolius by repeated column chromatography on silica, Sephadex LH-20 and structurally identified by spectral analysis. Results: The compounds were identified as chrysophanol (1), physcion(2), β-sitosterol (3), 3-oxotirucalla-7,24-dien-21-oic acid (4), myricadiol (5), 19-α-hydroxy-3-acetyl-ursolic acid(6), N-benzoylphenylalaninyl-N-benzoylphenylalaninate (7), aurantiamide acetate (8) and euscaphic acid (9). Conclusion: Compounds 1 and 4–8 are isolated from this plant for the first time,and compounds 4–8 are found in plants of Rubus genus for the first time.
Objective: To investigate the chemical constituents from Cynanchum paniculatum. Methods: The constituents were isolated and purified by silica gel, Sephadex LH-20 column chromatography, and preparative TLC. Their structures were identified on the basis of spectral data and physiochemical characteristics. Results: 15 compounds were isolated from 70% ethanol extract and identified as β-sitosterol (1), β-daucosterin (2), mudanoside A (3), paeonolide (4), santamarin (5), paeonol (6), annobraine (7), laricircsinol (8), α-asarone (9), 7-angelyheliotridine (10), β-amyrin (11), uridine (12), kaempferol-3- O-β-D-glucopyranosyl (1 →2)- α-L-arabinopyranoside (13), kaempferol-7- O-(4″,6″-di-p-hydroxycinnamoyl-2″,3″-diacetyl)- β-D-glucopyranoside (14), and (2 S, E)-N-[2-hydroxy-2-(4-hydroxyphenyl) ethyl]ferulamide (15). Conclusion: Compounds 4,6,8,11,12 and 15 are isolated from this plant for the first time, Compounds 5 and 14 are firstly isolated from the plants of Cynanchum genus.
Objective: To isolate and identify the phenylpropanoids from Saussurea hieracioides. Methods: The compounds were isolated by chromatographic and their structures were elucidated on the basis of spectra data. Results: Seven compounds were isolated and identified as umbelliferone (1), scopoletin (2), caffeic acid (3), esculetin (4), skimmin (5), scopolin (6) and chlorogenic acid (7), respectively. Conclusion: Compounds 3, 4 and 7 were isolated from this plant for the first time.
