China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline.
Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study.
The journal is included in CA, JST and CSCD.
Honorary Editor-in-Chief Xiao Peigen Editor-in-Chief Wang Yongyan
Associate Editors Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board Cai Shaoqing, Chen Shilin
The 70% ethanol extract of the whole plant of
Souliea vaginata was purified by multi-chromatographic methods including macroporous resin, silica gel, Sephadex LH-20, and C
18-reversed-phase column chromatography. A new spirocyclic cycloartane triterpenoid was isolated and identified as (16
R*,20
R*,23
S*,24
R*,25
S*)-16,23:23,26-diepoxy-15α,24,25-trihydroxy-9,19-cycloart-3
β-
O-
β-D-xylopyranoside (1), and named as soulieoside S. Its planar structure and relative configuration were determined by spectroscopic techniques including 2D NMR and HR-ESI-MS. As one of the main components of
S. vaginata, compound 1 was evaluated for its anti-inflammatory activity by a lipopolysaccharide (LPS)-stimulated NO production model in RAW264.7 macrophages, but it didn’t show NO production inhibitory effect.
To assess the effectiveness and safety of Xueshuantong (lyophilized powder) injection in the treatment of unstable angina pectoris. Literatures were retrieved from China National Knowledge Infrastructure (CNKI), Wanfang Data Knowledge Service Platform, Chongqing Weipu Database for Chinese Technical Periodicals (VIP), Chinese Biomedical Literature Service System (SinoMed), Cochrane Library, National Center for Biotechnology Information by National Library of Medicine, the US (PubMed) and EMbase. The retrieval time was set from their inception to April 28, 2019. The assessment of ethodological quality was based on the Cochrane Handbook 5.1, and the data were analyzed using Rev Man 5.3 software. A total of 38 RCTs involving 4 074 patients were included. The included trials were all of low quality. Xueshuantong (lyophilized powder) injection combined with routine basic treatment (RBT) was superior to RBT alone in alleviating clinical symptoms, with statistically significant differences between the groups (RR = 1.19, 95% CI [1.15, 1.24]). Xueshuantong (lyophilized powder) injection combined with RBT was better than RBT alone in alleviating angina pectoris symptoms (RR = 1.23, 95% CI [1.18, 1.29]). Xueshuantong (lyophilized powder) injection combined with RBT reduced the consumption of nitroglycerin, which was more effective than RBT alone, with statistically significant difference between the groups (RR = 1.64, 95% CI [1.23, 2.19]). Xueshuantong (lyophilized powder) injection combined with RBT decreased hs-CRP and FIB levels, which was more effective than RBT alone, with statistically significant differences between the groups (MD = −0.48, 95%CI [−0.57, −0.40]), and (RR = −0.45, 95% CI [−0.68, −0.21]), respectively. However, there was no statistical difference between groups in the incidence of myocardial infarction. The reported adverse effects of Xueshuantong (lyophilized powder) injection were mainly dizziness, headache, skin itching and gastrointestinal symptoms. Xueshuantong (lyophilized powder) injection combined with RBT alleviated unstable angina pectoris. However, due to the low quality of the included studies, further well-designed multicenter and large-scale RCTs are still needed to evaluate the efficacy of Xueshuantong (lyophilized powder) injection.
This study was to investigate the hypoglycemic effect of wogonoside to improve hepatic insulin resistance (IR) and its relative anti-inflammatory mechanism. The stable IR-HepG2 cell model was established by the combination of 1 × 10
−9mol·L
−1insulin and 3.75 × 10
−6mol·L
−1dexamethasone for 48 h. The changes of glucose consumption in IR-HepG2 cells with different concentrations of wogonoside (1, 5, 10, 20, and 50 μmol·L
−1) at different time points (30, 36, 48, and 54 h) were detected by glucose oxidase assay to determine the optimal onset time. Glycogen content and cell viability were respectively detected by ketone method and CCK-8 method. Cryptothermal protein 3 (NLRP3), suppressor of cytokine signaling 3 (SOCS3), Toll-like receptor 4 (TLR4), nuclear factor (NF-κB), interleukin (IL-1β), IL-6, tumor necrosis factor (TNF-α) involving in the inflammatory signaling pathway, as well as leptin, Ob-R, p-IRS2/IRS2, p-PI3K/PI3K (p85), p-Akt/Akt and glucose transporter (GLUT1/2/4) involving in the insulin signaling pathway were detected in IR-HepG2 cells by Western blot. Results showed that 20 and 50 μmol·L
−1 wogonoside significantly up-regulated the glucose consumption of IR-HepG2 cells (
P < 0.001) as compared with IR model group, and the optimal onset time was 48 h. Wogonoside had no obvious effect on the cell viability of HepG2 cells. Further studies showed that 20 and 50 μmol·L
−1wogonoside respectively increased the glycogen content of IR-HepG2 cells after 48 h treatment, especially in 50 μmol·L
−1group (
P < 0.001). Compared with IR model group, wogonoside not only inhibited the protein expression of inflammatory nuclear transcriptional factors NLRP3, SOCS3, TLR4, NF-κB, but also decreased the expression of downstream inflammatory effect factors IL-1β, IL-6 and TNF-α. In addition, wogonoside elevated Ob-R, p-IRS2/IRS2, p-PI3K/PI3K (p85), p-Akt/Akt and GLUT1/2/4 protein expression, whereas it suppressed leptin expression that was regulated by SOCS3. Wogonoside could promote glucose uptake and increase glycogen content to enhance insulin sensitivity in IR-HepG2 cells. The hypoglycemic effect may be related to the intervention of NLRP3/SOCS3-TLR4-NF-κB inflammatory pathway and decrease of inflammatory factor expression.
The objectives of study were to explore the effect of exogenous methyltestosterone (MT) on the growth and gonadal development of overwintering
Whitmania pigra. Before overwintering, 0.1, 1.0, 10.0, 100.0, and 150.0 μg·L
−1 MT was added to the aquaculture water for six weeks. The changes of growth performance, gonad index, endogenous steroid hormone level and internal quality were measured after hibernate for 60 days. Then the tissue slice technique was used to observe the testis (ovary) of
Wh. pigra. The results showed that the body weight, survival rate, and gonad index increased first and then decreased with the increase of exogenous MT concentration; the male gland index was found the highest when the concentration of MT was 10.0 μg·L
−1and the female gland index was the highest when the concentration of MT was 1.0 μg·L
−1. The survival rate of
Wh. pigra peaked at the concentration of MT 10.0 μg·L
−1. The weight reached a peak at the MT concentration of 100.0 μg·L
−1(
P < 0.05). The number of primary spermatocytes in the testis was negatively correlated with the concentration of exogenous MT. The number of secondary spermatocytes and spermatids increased first and then decreased. The concentration of secondary spermatocytes was the highest when the concentration of MT was 100.0 μg·L
−1. The number and volume of egg cells in the ovary and the yolk granules increased first and then decreased with the increase of exogenous MT concentration, and the highest values were observed at the MT concentration of 100.0 μg·L
−1. The endogenous steroid hormone of
Wh. pigra increased first and then decreased with the increase of exogenous MT concentration. The concentration of androgen and progesterone was the highest in 100.0 μg·L
−1MT treatment (
P < 0.05), and the concentration of estrogen was found the highest in 10.0 μg·L
−1MT treatment (
P < 0.05). After the exogenous MT was added,
Wh. pigra moisture content, acid-insoluble ash content, pH and anti-thrombin activity met the quality criterion of Hirudo in
Chinese Pharmacopoeia (2015 edition). In conclusion, the short-term addition of 1.0–100.0 μg·L
−1 exogenous MT before hibernation could promote the growth, the development of spermatids and the antithrombin activity of
Wh. pigra.
This study was aimed to investigate the mechanism of Danzhi Jiangtang Capsules (DJC) in the treatment of diabetic macroangiopathy in Goto-Kakizaki (GK) rats. The diabetic macroangiopathy rat model was induced by feeding high-fat and high-sugar combined with endothelial nitric oxide synthase (NOS) inhibitor N-nitro-L-arginine methyl ester (L-NAME) (0.1 g·L
−1·d
−1). According to the random number table, the model rats were randomly divided into the model group, DJC groups (1 260, 630, and 320mg·kg
−1), atorvastatin group (105 mg·kg
−1) and metformin group (10 mg·kg
−1), with 12 rats in each group. The rats received intragastric administration for eight weeks. Twelve Wistar rats were selected as the normal control. The changes in the body weight, water intake, blood glucose, plasma total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-C), low density lipoprotein (LDL-C), interleukin (IL-1
β), IL-6, tumor necrosis factor (TNF-
α), nitric oxide (NO), endothelin (ET-1) were observed in these rats. Aortic tissue was taken and the pathological changes were observed by HE staining. RT-PCR was used to detect the mRNA levels of IL-1
β, IL-6, and TNF-
α in rat aorta. The stem loop RT-PCR was conducted to detect the levels of miRNA-126, miRNA-155, miRNA-146a, and miRNA-21 in rat plasma and aortic tissue. The canonical correlation between miRNAs and inflammatory factors was then analyzed. The results showed that DJC increased the rat body weight, lowered water intake, reduced the random blood glucose, reversed the rat aorta tissue damage, reduced serum TC, TG, LDL-C, ET-1, IL-1
β, IL-6, and TNF-
α, as well as miRNA-155, miRNA-146a and miRNA-21 levels in serum, elevated plasma HDL-C, NO content, reduced the aorta mRNA of IL-1
β, IL-6, TNF-
α, and the miRNA-155, miRNA-146a and miRNA-21, elevated miRNA-126 expression in aorta. Aortic miRNA-126, miRNA-155, miRNA-146a and miRNA-21 expression levels were typically correlated with the expression of inflammatory factors, among which miRNA-126 was negatively correlated, miRNA-155, miRNA-146a and miRNA-21 were positively correlated with the factors. These results suggested that DJC had therapeutic effects on diabetic vascular complications, and the mechanism of action might be related to its regulation of miR-NA-126, miRNA-155, miRNA-146a and miRNA-21 levels, as well as the reduction of inflammatory factors and alleviation of vascular inflammatory response.
To evaluate the pharmacodynamic effect of Four-Ingredients Decoction on blood deficiency in mice under multidimensional pharmacodynamic indexes by factor analysis. The mouse blood deficiency model was established with cyclophosphamide combined with acetophenone. Mouse viscera index, white blood cell, red blood cell, hemoglobin, platelet counts in whole blood, serum granulocyte-macrophage colony-stimulating factor, macrophagecolony-stimulating factor, promotion erythropoietin, interleukin-3 and interleukin-6 were used as indexes to characterize the blood-tonifying effect of Four-Ingredients Decoction. The pharmacodynamic effect of Four-Ingredients Decoction on blood deficiency model was evaluated comprehensively by factor analysis. Four common factors were extracted from 14 pharmacodynamics indexes through the factor analysis, namely blood phase factor, viscera index, hematopoietic regulatory factor 1-spleen index and hematopoietic regulatory factor 2-viscera index. The cumulative contribution rate of variance reached 86.581%. The comprehensive score of factor analysis showed that Four-Ingredients Decoction had the best effect on blood replenishment, and it is significant compared with the model group (
P < 0.01). The effect of alcohol precipitation of Four-Ingredients Decoction was slightly decreased. The study showed that Four-Ingredients Decoction had the best blood-tonifying effect, followed by water decoction and traditional decoction. Alcohol precipitation had the worst effect. Factor analysis can be used for the comprehensive evaluation of blood deficiency mouse model, and is a suitable evaluation method for animal model for multi-dimensional multistage complex data analysis. It provides a new model to evaluate the efficacy of multidimensional data in the future.
The effectiveness and safety of Xiyanping Injection in the treatment of acute bronchitis in children was evaluated. The research systematically retrieved four Chinese databases ( namely CNKI, VIP, Wan Fang, Sino Med) and four English databases ( namely EMbase, Cochrane Library, Medline, Clinical Trail.gov). The retrieval time ranged from the commencement of each database to April, 2019. According to pre-set inclusion criteria and exclusion criteria, randomized controlled trials ( RCTs) of Xiyanping Injection in the treatment of acute bronchitis in children were screened out. The quality assessment of the included studies was performed using the “Cochrane Bias Risk Assessment” tool, and the Meta-quantitatively analysis on the included studies was performed using Rev Man 5.3 software. A total of 648 articles were retrieved, and 10 studies were finally included. Except for one multi-arm test, the total sample size was 1 260, including 630 cases in the test group and 630 cases in the control group. The overall quality of the included study was not high. The results of Meta-analysis showed that in terms of antipyretic time, Xiyanping Injection combined with conventional treatment was superior to conventional treatment in the shortening of fever time in children with acute bronchitis (MD = −0.94, 95%CI [−1.18, −0.70],
P < 0.000 01); in the overall efficacy, Xiyanping Injection combined with conventional treatment was superior to conventional treatment (RR = 1.34, 95% CI [1.26, 1.42],
P < 0.000 01) and Yanhuning Injection + conventional treatment (RR = 1.28, 95%CI [1.19, 1.38] ,
P < 0.000 01); descriptive analysis showed that Xiyanping Injection was excellent in the overall efficacy in treating acute bronchitis in children. The differences between the two groups were statistically significant (
P < 0.000 1). The adverse reactions included in the study were mild adverse reactions, with no impact on treatment. Based on the results of this study, Xiyanping Injection combined with conventional treatment or other Western medicine had a certain effect on acute bronchitis in children, especially in improving the overall efficacy of acute bronchitis in children. No serious adverse reactions were observed. And in the time of fever, disappearance time of cough and sputum, disappearance time of lung sound, Xiyanping Injection + conventional treatment or Western medicine therapy was better than conventional treatment or Western medicine therapy. However, the small size of included studies, the low quality of the included studies, and the existence of publication bias and the low quality of the evidence had impacts on the reliability of the conclusion. Therefore, more large-sample, multi-center, well-designed, rigorous randomized controlled trials with best case reports are required to further verify the efficacy and safety.
