China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline.
Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study.
The journal is included in CA, JST and CSCD.
Honorary Editor-in-Chief Xiao Peigen Editor-in-Chief Wang Yongyan
Associate Editors Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board Cai Shaoqing, Chen Shilin
Chemical constituents of the Fufang Huangbai Ye were analyzed and identified by UPLC-ESI-LTQ-Orbitrap MS. The analysis was performed on an Waters HSS T3 reverse phase column (2.1 mm × 100 mm, 1.8 μm). The mobile phase consisting of 0.1% aqueous formic acid (A) and acetonitrile (B) was used with gradient elution, and the flow rate was 0.3 mL·min
−1. Based on the information of the accurate mass, the multistage fragment ions, the mass spectrometric data of the standard substance and the relative reference literature, the structure of the chemical constituents in Fufang Huangbai Ye were identified. Based on the identified compounds, network pharmacology study, including target prediction, functional enrichment, and molecular docking was applied to screen out the main active substances for treatment of diabetes foot and explore the potential mechanism. The results showed that a total of 138 compounds were identified, including 28 alkaloids, 16 flavonoids, 11 phenylethanoid glycosides, 9 cycloolefins, 11 cyclohexylethanol derivatives, 28 phenolic acids and derivatives, 3 lignans, 4 terpenes, 28 volatile oils and the others. Further, 36 active substances for diabetes foot were screened out, and the functional enrichment showed the potential mechanism of Fufang Huangbai Ye were mainly seven functional items including inflammatory response, growth factor activity. This study combining the UPLC-LTQ-Orbitrap-MS technology and the network pharmacology provide a useful reference and basis for active compounds, quality control markers and the pharmacological mechanism of Fufang Huangbai Ye for diabetic foot treatment.
To isolate and identify secondary metabolites of marine-derived
Streptomyces sp. MDW-06, the isolations and purifications of compounds were performed by means of column chromatography over silica gel. And their structures were elucidated through the spectroscopic analysis of MS, NMR and specific rotations.The bioactivities were assayed by paper diffusion and DPPH method. From the fermentation broth of marine-derived
Streptomyces sp. MDW-06, six compounds (
1–6) were isolated and identified as streptopentanoic acid (
1), germicidin A (
2), germicidin B (
3), isogermicidin A (
4), isogermicidin B (
5) and oxohygrolidin (
6) , respectively. Compound
1 is a new compound. Compound
1 shows DPPH radical scavenging activity with 36.4% at 100 mg·L
−1.
To construct a quality management model for the whole industry chain of compound Danshen Tablets, and quality control system for all key links in the production of compound Danshen Tablets, three batches of mix standards were prepared, and three sets of relative correlation factors between salvianolic acid B and other phenolic acids were calculated in parallel with salvianolic acid B as internal reference substance in this study. Finally, the correlation factors were obtained on average. The mass transfer process was studied by optimizing the concentration of
Salvia miltiorrhiza extract. The results showed that RSD among three sets of relative correlation factors ranged from 1.7% to 4.1%, with no significant difference between the quantitative results of two methods. In addition, the mass transfer study showed that with the rise of the concentration temperature, the content of phenolic acid components was changed, which had significant effect on the salvianolic acid B at over 80 °C. It was suggested to rationally control the concentration temperature during the industrial production. The results of this study provide a methodology for the establishment of the quality control system for the whole industry chain of compound Danshen Tablets, and quality control methods for the improvement of the quality of medicinal materials and finished medicine products.
The family of flavonoid 3-
O-glucosyltransferase catalyzes the modification of anthocyanin from unstable-structure to stable-structure. In this study, based on homology cloning and transcriptome library, we isolated the full-length cDNA of UDP-glucose: flavonoid 3-
O-glucosyltransferase (named
SmUF3GT) from the flower tissues of
S. miltiorrhiza. This gene was consisted of 1 353 bp open reading frames (ORF) encoding 450 amino acids. And the
SmUF3GT protein was performed for the bioinformatic analysis. Our results showed that the protein was preliminary localized in the Golgi and peroxisome of cytosol, as well as plasma membrane and cell nuclear. QRT-PCR analyses indicated that
SmUF3GT expressed differently in all tissues and organs but roots of
S. miltiorrhiza and
S. miltiorrhiza f.
alba. During floral development, the expression of
SmUF3GT showed a trend of rising fist and then down in purple-flower Danshen, whereas decreasing sharply fist and then slowly in white-flower Danshen. The present study provides basic information for further research on the network of synthesis and accumulation of flavonoids in
S. miltiorrhiza.
The endophytic fungi from root, stem, branch, and leaf of
Scrophularia ningpoensis were isolated from Zhejiang, whether these strains could yield harpagide or harpagoside were tested by HPLC and LC-MS. According to the morphological characteristic and the similarity of the nucleotide sequence of internal transcribed spacer ( ITS) between rDNAs, the strains producing harpagide or harpagoside were identified. The results showed that 210 strains were isolated from the samples, which were classified into 9 orders, 13 families and 17 genera by morphological study. Harpagide was detected in endogenous fungi ZJ17 and harpagoside was detected in endogenous fungi ZJ25 by HPLC coupled with LC-MS. ZJ17 was identified as
Alternaria alternate and ZJ25 was identified as
A. gaisen by its morphology and authenticated by ITS ( ITS4 and ITS5 regions and the intervening 5.8S rDNA region).
The aim of this paper was to investigate the therapeutic effects of psoralen and isopsoralen in the treatment of lipid accumulation in LO2 cells and its underlying mechanisms. The non-alcoholic fatty liver disease models were established in human LO2 cells by palmitic acid (PA). The cells were then treated with psoralen and isopsoralen. The intracellular level of triglycerides (TG) and total cholesterol (TC) content, and the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the supernatant were determined by enzymatic assay. The expression of proinflammatory cytokines (IL-6, TNF-α) and chemokines (IL-8, MCP-1) in the supernatant was determined by ELISA. Western blot was performed to determine the intracellular protein expression of nuclear factor (NF-κB), phosphorylated p65 (p-p65), nonphosphorylated p65, and transforming growth factor-β1 (TGF-β1). According to the results, compared with the model group, the intracellular levels of TG and TC in psoralen intervention group were decreased (
P < 0.01,
P < 0.05). The levels of ALT, AST, proinflammatory cytokines and chemokines in the cell supernatant were decreased (
P < 0.01,
P < 0.05). The p-p65/p65 ratio and protein expression of TGF-β1 were also significantly decreased (
P < 0.01,
P < 0.05) in the psoralen intervention group. As compared with the model cells, the intracellular level of TG was not significantly changed, but the levels of all the other parameters were reduced (
P < 0.01,
P< 0.05) in the cells of isopsoralen intervention group. The therapeutic effect of psoralen was better than that of isopsoralen (
P < 0.01,
P < 0.05). It is concluded that psoralen could ameliorate the steatosis of LO2 cells induced by PA. Both psoralen and isopsoralen are effective in ameliorating the LO2 cells injury induced by PA, reducing inflammation via inhibiting the activation of NF-κB and down-regulating the expression of TGF-β1.
Cordyceps is one of the most valuable traditional Chinese medicines. There are various counterfeits in markets because of high price and limited output. In this study, 116
Cordyceps, 146 hosts and 29 related products were collected and detected by using normal DNA barcoding technology and specific PCR method. The results indicated that
Cordyceps and its adulterants could be distinguished from each other through DNA barcoding technology based on ITS and COⅠ sequences. Two pairs specific primers ITSSF1/ITSSR1 and ITSSF2/ITSSR2 were developed to amplify 297 bp and 136 bp ITS regions of
Cordyceps sinensis, respectively. It could be used to identify
C. sinensis specifically and rapidly. Furthermore, specific primers ITSSF1/ITSSR1 and ITSSF2/ITSSR2 combined with ITS and COⅠ sequences could differentiate powder
Cordyceps from fermentation mycelia and could identify related products. Therefore, the method developed from this study could be applied to identify the powder of
Cordyceps from fermentation mycelia and related products efficiently.
