China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline.
Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study.
The journal is included in CA, JST and CSCD.
Honorary Editor-in-Chief Xiao Peigen Editor-in-Chief Wang Yongyan
Associate Editors Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board Cai Shaoqing, Chen Shilin
Near infrared spectroscopy (NIRS) was used for rapid quantitative analysis of saponins in Pien Tze Huang lozenges and powders. The near infrared spectra of Pien Tze Huang were collected, and the contents of notoginsenoside R
1, ginsenoside Rg
1 and ginsenoside Rb
1 in Pien Tze Huang were determined by high performance liquid chromatography (HPLC) as the reference values. Then the near infrared spectra of the samples were associated with the reference values to establish the quantitative analysis models by using partial least squares (PLS) method. Finally, the models were verified by unknown samples. The results showed that root mean square error of cross-validation (RMSECV) of R
1, Rg
1, Rb
1 and the total content was 0.095 1, 0.555, 0.414, 0.960 mg·g
−1 for the lozenge models, 0.085 6, 0.443, 0.405, 0.913 mg·g
−1 for the powder models. After external validations, root mean square error of prediction (RMSEP) of R
1, Rg
1, Rb
1and the total content was 0.111, 0.274, 0.276, 0.807 mg·g
−1 for the lozenge models, 0.059 2, 0.322, 0.327, 0.705 mg·g
−1 for the powder models. The averages of relative standard deviation between the predicted values and the chemical measured values were all less than 2.0%. According to the results of paired-
t tests at the level of α = 0.05, there were no significant differences between the predicted values and the measured values. The established quantitative analysis models can be used to predict the contents of saponins in Pien Tze Huang accurately and the proposed method is simple, fast, non-destructive and environmentally friendly for the rapid detection and quality control of saponins in Pien Tze Huang.
To study the effect of modified Buyang Huanwu Decoction on the hemorrhagic transformation after intravenous thrombolysis with recombinant tissue type plasminogen activator (rt-PA) in patients with super early cerebral infarction (onset time < 4.5 h). From March 2016 to July 2018, 212 cases with super early cerebral infarction were selected from the Encephalopathy Ward of the First Affiliated Hospital of Henan University of Chinese Medicine, and divided into two group according to the randomized complete blocks designs: the control group (106 cases) and Chinese medicine group (106 cases). The patients in the control group were treated with rt-PA intravenous thrombolysis, while those in the Chinese medicine group were treated with modified Buyang Huanwu Decoction in addition to the therapies adopted in the control group. Both groups were treated for 14 days. Neurological deficit score, serum matrix metalloproteinase-9 (MMP-9), neuron specific enolase (NSE), vascular endothelial growth factor (VEGF), and plasma cellular fibronectin (c-FN) levels, the incidence of hemorrhagic transformation, clinical efficacy, as well as adverse drug reactions before and after treatment were compared between the two groups. According to the findings, on the 14
thday after treatment, the rank sum test of the grade data showed that the clinical efficacy of the Chinese medicine group was better than that of the control group (
Z = −2.033,
P = 0.042). The
χ2 test revealed that the total efficiency of the Chinese medicine group was higher than that of the control group (
χ2 = 4.895,
P = 0.027). The hemorrhagic transformation rate of the Chinese medicine group was lower than that of the control group within14 days of treatment (
χ2 = 3.962,
P = 0.047). The MMP-9 levels in the Chinese medicine group were lower than those in the control group on the 3
rd, 5
th, 7
th, 10
th, and 14
th days after treatment (
t = −2.474, −3.022, −5.163, −6.998, and −9.821;
P = 0.014, 0.003, 0, 0, and 0). The improvements of c-FN, NSE, VEGF, and NIHSS scores in the Chinese medicine group were superior to those of the control group after 14 days of treatment (
t = −2.343, −3.187, −2.129, and −3.105;
P = 0.020, 0.002, 0.034, and 0.002). No obvious adverse reactions of modified Buyang Huanwu Decoction were observed during 14 days of treatment. Modified Buyang Huanwu Decoction could reduce the expression of MMP-9, c-FN, NSE and VEGF after intravenous thrombolysis with rt-PA for super early cerebral infarction, and decrease the hemorrhagic transformation rate after thrombolysis, with high safety.
This paper was aimed to investigate the inhibitory effect of aconitine (AC) on angiotensin Ⅱ (Ang Ⅱ)-induced H9c2 cell hypertrophy and explore its mechanism of action. The model of hypertrophy was induced by Ang Ⅱ (1 × 10
−6 mol·L
−1), and cardiomyocytes were incubated with different concentrations of AC. Western blot was used to quantify the protein expression levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP),
β-myosin heavy chain (
β-MHC), and α-smooth muscle actin (α-SMA). Real-time quantitative PCR (qRT-PCR) was used to quantify the mRNA expression levels of cardiac hypertrophic markers ANP, BNP and
β-MHC. In addition, the fluorescence intensity of the F-actin marker, an important component of myofibrils, was detected by using laser confocal microscope. AC could significantly reverse the increase of total protein content in H9c2 cells induced by Ang Ⅱ; the qRT-PCR results showed that AC could significantly inhibit the ANP, BNP and
β-MHC mRNA up-regulation induced by Ang Ⅱ. The Western blot results showed that AC could significantly inhibit the ANP, BNP and
β-MHC protein up-regulation induced by Ang Ⅱ. In addition, the F-actin expression induced by Ang Ⅱcould be inhibited by AC, and multiple indicators of cardiomyocyte hypertrophy induced by Ang Ⅱ could be down-regulated, indicating that AC may inhibit cardiac hypertrophy by inhibiting the expression of hypertrophic factors, providing new clues for exploring the cardiovascular protection of AC.
In order to improve the quality control level of Ligustri Lucidi Fructus (LLF) and to explore the changes of chemical components after processing, the HPLC method for fingerprint and simultaneous determination of the major polar components in LLF were established. The octadecylsilane bonded silica gel was used as the stationary phase, with acetonitrile as the mobile phase A and 0.2% formic acid as the mobile phase B in a gradient elution procedure at a flow rate of 1.0 mL·min
−1. The detection wavelength was set at 280 nm and the column temperature was 25 °C. There were 22 common peaks, 20 of which were selected from the fingerprint of LLF and its wine-steamed product, respectively, and 14 chromatographic peaks were identified with reference substances. With the same chromatographic conditions, seven components were quantitatively analyzed and the results of system adaptability and methodology investigation all met the requirements of content determination. Compared with the crude LLF, the content of 5-hydroxymethyl furfural and salidroside significantly increased in wine-steamed LLF, while the contents of iridoid glycosides generally decreased. The method provided a basis for quality control of LLF and its processed products as well as the related preparations.
Rhizosphere bacteria play a vital role in plant nutrition absorption, growth and disease resistance. In this study, high-throughput sequencing technology was used to analyze the rhizosphere bacterial communities of
Salvia miltiorrhiza and
S. miltiorrhiza f.
alba. Moreover, the function of dominant rhizosphere bacterial communities was analyzed. We found that Sphingobacteriales, Sphingomonadales and Nitrosomonadaceae were both dominant and specific bacteria in the rhizosphere of
S. miltiorrhiza. The main functions of dominant rhizosphere bacteria communities in both species include promoting transformation of soil nutrients, improving plant immunity and ability of stress tolerance. This study was first compare the rhizobacterial community structure and function of
S. miltiorrhiza and
S. miltiorrhiza f.
alba, which provides a new theoretical reference for studying the rhizosphere mechanism of healthy
S. miltiorrhiza planting in the future.
Because the red and bright color of corolla is the main indicator for the quality assessment of good safflower, the dyed safflower is sometimes found at the herbal market, which influences the quality and efficacy of this herb. A total of 127 safflower samples were therefore collected from different cultivating areas and herbal markets in China to develop a rapid method to identify the dyed safflower. Near-infrared spectroscopy (NIRS) combined with characteristic identification, high performance liquid chromatography (HPLC), principal component analysis (PCA) and partial least squares (PLS) regression analysis were employed to differentiate safflower from dyed safflower samples, and further quantify the levels of the 6 dyes, i.e., tartrazine, carmine, sunset yellow, azorubine, acid red 73 and orange Ⅱ in the dyed safflower. The results indicated that the 50 safflower samples and 77 dyed safflower samples were located at different regions in PCA cluster diagram by NIR spectra. Tartrazine, carmine and sunset yellow were found in the 77 dyed safflower samples with the amounts of 0.60–3.66, 0.11–1.37, 0.10–0.71 mg·g
−1, respectively. It indicated that the three dyes were the common and main dyes in the dyed safflower. However, azorubine, acid red 73 and orange Ⅱ were not detected in all herb samples. A total of 62 dyed safflower samples were chosen as calibration samples to develop the model for estimating the amount of dyes in dyed safflower. The estimating accuracy was verified by another 15 dyed safflower samples. The values of tartrazine, carmine and sunset yellow in dyed safflower samples were compared between the NIRS and HPLC methods. Each value of mean absolute difference (MAD) was less than 5%. The correlation coefficients of tartrazine, carmine and sunset yellow were 0.970, 0.975, 0.971, respectively. It indicated the data quantified by NIRS and HPLC were consistent. It is concluded that NIRS can not only differentiate safflower from dyed safflower, but also quantify the amount of the dyes. NIRS is suitable for rapidly identify the quality of safflower.
To explore the anti-platelet aggregation and anti-thrombotic mechanisms of Trichosanthis Fructus combined with aspirin based on network pharmacology and the validation of arteriovenous bypass model in rats. The databases of Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), Drug Repositioning and Adverse Drug Reaction Chemical-Protein Interactome (DRAR-CPI), Universal Protein Resource (Uniprot) and the Database for Annotation, Visualization, and Integrated Discovery (DAVID) were used to predict protein targets and analyze the biological pathway and signaling pathway of Trichosanthis Fructus combined with aspirin. The effects of Trichosanthis Fructus pellets, aspirin pellets and their combination on thromboxane B
2 (TXB
2), 6-keto prostaglandin F
1
α (6-keto-PGF
1
α), and cyclic adenosine monophosphate (c AMP) in the rat thrombotic model were studied. Based on network pharmacology, 12 components of aspirin and Trichosanthis Fructus, including hydroxygenkwanin, quercetin, and adenosine, were found to exert the anti-platelet aggregation and anti-thrombosis effects through nine common protein targets, such as SRC, RAC1, MAPK14, MAPK1, AKT1, and 14 common signaling pathways, such as VEGF signaling pathway. After the intervention with Trichosanthis Fructus pellets combined with aspirin pellets, the vascular endothelial growth factor (VEGF) signaling pathway could be activated to inhibit platelet aggregation and improve vascular endothelial function, which verified the results of the network pharmacology, and explained the mechanism of Trichosanthis Fructus pellets combined with aspirin pellets in anti-platelet aggregation and anti-thrombosis.
