China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline.
Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study.
The journal is included in CA, JST and CSCD.
Honorary Editor-in-Chief Xiao Peigen Editor-in-Chief Wang Yongyan
Associate Editors Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board Cai Shaoqing, Chen Shilin
Gastrodia elata B1., a traditional Chinese medicine, was frequently applied as a cure for headache or migraine due to its efficacy in extinguishing wind, stopping convulsions, pacifying liver, suppressing yang, expelling wind and dredging collaterals. A proposal has been made that
G. elata should be added to
The List of Substances that Are Traditionally Both Food and Chinese Medicinal Materials. The dry
G. elata was commonly used in clinic, and its efficacy and mechanism have been explored. However, fresh
G. elata which was added to herbal cuisine very often lacks corresponding research. The interaction between diet, intestinal flora and host as well as its effects on human health has aroused much attention from the scholars. This research sequenced the 16 rRNA of mouse cecal contents on Mi Seq platform to understand the effect of fresh
G. elata. The results revealed that multiple probiotics grew after intake of fresh
G. elata extract, including
Ruminiclostridium,
Butyricicoccus, and
Parvibacter. By contrast, some pathogens or potential pathogens, such as
Escherichia/Shigella and
Parasutterella were decreased. These suggested that fresh
G. elata played a certain role in regulating the gut microbiota of mouse. In particular, the low-dose fresh
G. elata extract could restructure the microbiota apparently. Our result reveals that microbiota might be a new target for
G. elata extract, which will provide an important basis for further research on the interaction between gut microbiota and pharmacological activity of
G. elata.
The phenolic constituents of
Wikstroemia chamaedaphne were investigated by various column chromatographic methods including silica gel, Sephadex LH-20, ODS and preparative HPLC, and their chemical structures were identified based on physico-chemical properties and spectral analyses. Thirteen phenolic compounds were isolated and elucidated, including five flavonoids: luteolin 7-
O-
β-D-glucopyranoside (
1), luteolin 4'-
O-
β-D-glucopyranoside (
2), kaempferol 3-
O-
β-D-glucopyranoside (
3), chrysoeriol 4'-
O-
β-D-glucopyranoside (
4), chrysoeriol (5) ; and eight lignans: (−)-secoisolariciresinol (
6), acanthosessilin A (
7), (−)-nortrachelogenin (
8), (+)-isolariciresinol (
9), sesamin (
10), syringaresinol (
11), (+)-epipinoresinol (
12), and [3, 3', 4, 4'-tetrahydro-6, 6'-dimethoxy-3, 3'-bi-2
H-benzopyran]-4, 4'-diol (
13). Compounds
1,
3, 5–8,
10,
11 and
13 were obtained from the plant
W. chamaedaphne for the first time, and compounds
1,
5,
7,
10 and
13 were obtained from the
Wikstroemia genus for the first time.
Longshengzhi Capsule consisting of 12 herbs is widely used in clinically treating cerebral ischemia during recovery period. In this study, in order to investigate the consistency of different batches of Longshengzhi Capsules, a high performance liquid chromatography coupled to triple quadrupole mass spectrometry method (HPLC-QQQ/MS) was developed for the determination of 19 representative components in Longshengzhi Capsules within 9 min. Methodology validation indicated this method was simple, rapid, accurate, highly sensitive and reproducible, and it could be used for the content determination of components in Longshengzhi Capsules. The consistency analysis results showed that paeoniflorin and calycosin-7-glucoside in Longshengzhi Capsules had the highest content; RSD value of total content of 19 compounds was 5.2% and the RSD value of main compounds such as astragaloside and calycosin-7-glucoside was all less than 15%, reflecting good consistency among different batches. This study has provided a scientific method and basis for the quality control and consistency evaluation of Longshengzhi Capsules.
In this study, in-depth systematic evaluation of rat model with acute kidney injury (AKI) caused by renal arteriovenous ligation was conducted to better master and apply this model for drug research. Male SD rats of 2–3 months old were employed in this study. The left kidney was removed, and the right kidney received ligation for 40 min and reperfusion for 24 h. Serum creatinine (Crea), urea nitrogen (BUN) and the renal tissue sections were assayed as the basic indicators to evaluate their renal function. The mRNA expression levels of inflammatory necrosis factors and apoptotic factors were used to evaluate the mechanism of molecular pathophysiological changes. The results showed that the serum Crea and BUN caused by ligation of both renal arteries and veins were significantly higher than those of rats with renal artery ligation. After renal arteriovenous ligation for 40 min and reperfusion for 24 h in rats, the serum Crea of the rats varied from less than 100 μmol·L
−1 to more than 430 μmol·L
−1. Among them, five rats showed less than 100 μmol·L
−1 serum Crea, with 20 rats exhibiting 100-200 μmol·L
−1 serum Crea and 12 rats exhibiting more than 430 μmol·L
−1. Rats with serum Crea between 300–430 μmol·L
−1 accounted for 66.3% (122/184) of the total number of the experiment rats. After 72-h reperfusion, serum Crea in the 370–430 μmol·L
−1 group continued to increase, while the serum Crea levels in the Crea 200–300 μmol·L
−1 group and the Crea 300–370 μmol·L
−1 group were recovered quickly. No matter the serum Crea was elevated or decreased, the renal tubules showed pathological changes such as vacuolar degeneration or even necrosis. The mRNA expression levels of Toll-like receptor (TLR4) , tumor necrosis factor (TNF-
α) and interleukin (IL-6) in renal tissues were significantly up-regulated, and the elevation in the serum Crea 370–430 μmol·L
−1 group was most obvious. The study indicated that the renal arteriovenous ligation and reperfusion is an easy operation for induction of AKI model, and the serum Crea and BUN would continuously increase, which was beneficial to the observation of drug effects. This acute kidney injury is mainly related to inflammatory necrosis.
To research the correlation between accumulation of triterpenoids and expression of key enzymes genes in triterpenoid biosynthesis of
Alisma orientale, the study utilized UPLC-MS/MS method to detect eight triterpenoids content in the tuber of
A. orientale from different growth stages, including alisol A, alisol A 24 acetate, alisol B, alisol B 23 acetate, alisol C 23 acetate, alisol F, alisol F 24 acetate and alisol G, and then the Real time quantitative PCR was used to analyze the expression of key enzymes genes
HMGR and
FPPS in triterpenoid biosynthesis. Correlation analysis showed that there was a significant positive relation between the total growth of these eight triterpenoids and the average relative expression of
HMGR and
FPPS (
HMGR:
r = 0. 998,
P < 0. 01;
FPPS:
r = 0. 957,
P < 0. 05), respectively. Therefore, the study preliminarily determined that
HMGR and
FPPS genes could regulate the biosynthesis of triterpenoids in
A. orientale, which laid a foundation for further research on the biosynthesis and regulation mechanism of triterpenoids in
A. orientale.
A stable hepatoma cell line (Hep G2 cell) insulin resistance (IR) model was established and used to analyze the effect of effective components of Mori Folium in alleviating IR, and preliminary explore the mechanism for alleviating IR. The Hep G2 insulin action concentration and the duration of action were investigated using the glucose oxidase method (GOD-POD method) to establish a stable Hep G2 IR model. Normal control group, model group, Mori Folium polysaccharide group, Mori Folium flavonoid group and RSG group were set up to determine the glucose consumption. The effect of effective components in Mori Folium on Hep G2 IR was analyzed. The mRNA expressions of JNK, IRS-1 and PDX-1 in each group were detected by Real-time quantitative PCR (qRT-PCR). The protein expressions of p-JNK, IRS-1 and PDX-1 were detected by Western blot. And the mechanism of effective components of Mori Folium in alleviating IR was investigated. The results showed that the glucose consumption was significantly decreased in the IR cells after incubation with 25. 0 mg·L
−1 insulin for 36 h (
P < 0. 01), and the model was relatively stable within 36 h. Mori Folium polysaccharides and flavonoids both alleviated IR, among which Mori Folium flavonoids had better efficacy in alleviating Hep G2 IR (
P < 0.05). The qRT-PCR analysis showed that Mori Folium polysaccharides and flavonoids could inhibit JNK and IRS-1 mRNA expressions, while enhancing PDX-1 mRNA expression. Western blot analysis displayed that Mori Folium polysaccharides and flavonoids could inhibit p-JNK and IRS-1 protein expressions, while enhancing PDX-1 protein expression. Mori Folium polysaccharides and flavonoids can alleviate IR in Hep G2 cells, and its mechanism may be the alleviation of IR by inhibiting JNK signaling pathway.
The study aimed to establish an UPLC-MS/MS method for the determination of baicalin in rat plasma, in order to study the effect of PEG400 on the pharmacokinetics of baicalin and baicalein in normal and gut microbiota dysbiosis rats. Plasma was precipitated with ethyl acetate and determined by UPLC-MS/MS method, with genistein as an internal standard. In terms of specificity, linearity, range, accuracy, precision, and stability, the method was suitable for the determination of baicalin in plasma. The gut microbiota dysbiosis rat model was induced through the oral administration with lincomycin hydrochloride (5 g·kg
−1·day
−1) for one week. Samples of plasma of rats were obtained at different time points. After the rats were administrated with baicalin, baicalin and PEG400, baicalin in rats was detected by UPLC-MS/MS method, and pharmacokinetic parameters were calculated by DAS 3.2.2 software. The results showed that the
β-glucosidase activity and the number of colonies in the feces of gut microbiota dysbiosis rats induced by lincomycin hydrochloride were significantly reduced. The
Cmax and AUC
0-
t of the baicalin and PEG400 group in the intestinal flora were significantly lower than those in the normal rat treated with baicalin and PEG400. There was no significant difference in
Cmax and AUC
0-
t between the baicalin group and the baicalin + PEG400 group of gut microbiota dysbiosis rats. The
Cmax and AUC
0-
t of the normal rats in the baicalin group were significantly higher than those of the gut microbiota dysbiosis rats in the baicalin group and the baicalin + PEG400 group. There was no significant difference in
Cmax and AUC
0-
t between the normal rat in the baicalein and PEG400 group and the baicalein group. The
Cmax and AUC
0-
t of the baicalein group in the gut microbiota dysbiosis rats were lower than those of normal rats in the baicalein group, but significantly higher than those in the baicalein and PEG400 group. PEG400 could increase the absorption of baicalin in normal rats, but was ineffective in gut microbiota dysbiosis rats, with no impact on the absorption of baicalein in rats.
