China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline.
Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study.
The journal is included in CA, JST and CSCD.
Honorary Editor-in-Chief Xiao Peigen Editor-in-Chief Wang Yongyan
Associate Editors Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board Cai Shaoqing, Chen Shilin
The purpose of this experiment was to study the effects of different shading conditions on the growth, physiological characteristics and biomass allocation of
Polygonatum cyrtonema, so as to provide a theoretical basis for its cultivation. Different light environments (100%, 80%, 60% and 35% light transmittance) were simulated with shading treatments. Growth and photosynthetic indexes of
P.cyrtonema were measured and the variances were analyzed. The results showed that shading decreased superoxide anion radical (O
2−·) production rate and hydrogen peroxide (H
2O
2) accumulation, and maintained the activities of SOD, POD and CAT enzyme at a high level. Furthermore, the contents of chlorophyll a and chlorophyll b, net photosynthetic rate (
Pn), stomatal conductance (
Gs), transpiration rate (
Tr), maximal photochemical efficiency of photosystem Ⅱ (
Fv/
Fm), photochemical quenching index (qP) and effective quantum yield of photosystem II (
ΦPSⅡ) of
P. cyrtonema were increased while the intercellular CO
2 concentration (
Ci),
Fo and NPQ were decreased by shading. Shading was beneficial to
P.cyrtonema growth, and it could increase the total biomass in
P.cyrtonema. The allocation proportion of biomass on the aerial portion of
P.cyrtonema was increased but that in the underground parts was decreased with an increase in shading conditions. In this study,
P. cyrtonema could grow well in shading conditions, and 60% light transmission condition was most suitable for the growth of
P. cyrtonema, thus improving the rhizome yield and quality.
This research was aimed to evaluate the protective effect and potential mechanism of Yiqi Tongluo Particles (YQTLs). Firstly, an animal model of multiple cerebral infarction (MCI) with Qi deficiency and blood stasis was established. Rats were randomly divided into six groups: SHAM group, Vehicle group, Buyang Huanwu decoction original group (BYHWO), EGb761 group, high and low dose of YQTLs group. Rats underwent sleep deprivation after one week of MCI and the tongues and pulses of rats after six weeks of sleep deprivation were detected, followed by collecting blood to analysis the blood coagulation. Differential expression of angiogenesis associated proteins was examined using proteomic research and verified by immunohistochemical. Results showed that neurological function score was obviously declined, G and B value of tongue surface was increased significantly and the pulse distension, the activated partial thromboplatin time (APTT) as well as prothrombin time (PT) was recovered following YQTLs 7.56 g·kg
−1 treatment. Furthermore, G value of tongue surface, APTT and PT were also improved by YQTLs 3.78 g·kg
−1. The results of proteomic technology showed that proteins associated with angiogenesis were reversed compared with Vehicle group. Moreover, the expression of VEGFR2 from immunohistochemical was promoted after YQTLs treatment. The MCI with Qi deficiency and blood stasis was alleviated obviously following YQTLs treatment and the possible mechanism was that YQTLs may enhance angiogenesis during cerebral ischemia.
To explore the mechanism of
β-carboline alkaloids inhibiting the migration and invasion of SGC-7901 cells and its correlation with FAK gene expression, CCK-8 method was used to determine the inhibitory rate of
β-carboline alkaloids on the proliferation of gastric cancer SGC-7901 cells under different concentrations. The effect of
β-carboline alkaloids on the migration and invasion of SGC-7901 cells was used by Transwell compartment. Detection of mRNA and protein expression of FAK genes were used by qRT-PCR and Western blot. Then si-FAK-1051 recombinant plasmid was transfected into SGC-7901 cells. FAK gene silencing effect was identified by qRT-PCR and Western blot technique again. Finally, the effects of FAK gene silencing on proliferation and migration of gastric cancer SGC-7901 cells were detected by CCK-8 kit and Transwell chamber assay respectively. With the increase of the concentration of
β-carboline alkaloids, the inhibitory rate of SGC-7901 cells in human gastric cancer cells increased gradually, with IC
50 13.364 mg·L
−1. The number of SGC-7901 cells of Transwell compartment in the positive experimental group (5-FU, 5 mg·L
−1) and the
β-carboline alkaloids group decreased significantly (
P < 0.01) and the number of SGC-7901 cells in the
β-carboline alkaloids group was significantly lower than that in the positive experimental group (
P < 0.01). Compared with the blank control group, the mRNA and protein expression level of FAK genes in the positive experimental group was significantly lower than that in the experimental group of
β-carboline alkaloids (
P < 0.05). After transfection of si-FAK-1051 into gastric cancer SGC-7901 cells, the expression of mRNA and protein of FAK gene was significantly down regulated (
P < 0.05). SGC-7901 cell proliferation and cell migration ability also decreased significantly (
P < 0.05).
β-Carboline alkaloids are more effective than 5-FU in inhibiting migration and invasion of gastric cancer SGC-7901 cells, and the mechanism may be related to the inhibition of mRNA and protein expression of FAK gene by
β-carboline alkaloids.
Ten fractions (A-J) were prepared by separation of Longxue Tongluo Capsules (LTC) by using silica gel column chromatography and orthogonal experimental design, showing similar chemical profiles with different abundances of peaks. These ten samples were assessed with UHPLC-QE Orbitrap HRMS for 97 common peaks. For the pharmacological activity experiment, three kinds of in vitro cell models including lipopolysaccharide (LPS)-induced BV-2 microglial cells NO release model, oxygen-glucose deprivation/reoxygenation (OGD/R)-treated HUVEC vascular endothelial cells injury model, and OGD/R-treated PC-12 nerve cells injury model were employed to evaluated the bioactivity of each fraction. Based on the contribution of each identified component, grey relation analysis and partial least squares (PLS) analysis were performed to establish component-activity relationship of LTC, identify the potential active components. After that, validation of the potential active components in LTC was carried out by using the same models. The results indicated that 4 phenolic compounds including 7,4'-dihydroxyhomoisoflavanone, loureirin C, 4,4'-dihydroxy-2,6-dimethoxydihydrochalcone, and homoisosocotrin-4'-ol, might be the active components for anti-neuroinflammation effect; five phenolic compounds such as 3,5,7,4'-tetrahydroxyhomoisoflavanone, loureirin D, 7,4'-dihydroxyhomoisoflavanone, 7,4'-dihydroxyhomoisoflavane, and 5,7-dihydroxy-4'-methoxy-8-methyflavane, might have positive effects on the vascular endothelial injury; three phenolic compounds including 5,7,4'-trihydroxyflavone, 7,4'-dihydroxy-5-methoxyhomoisoflavane, and loureirin D, might be the active components in LTC against neuronal injury
To achieve a comprehensive understanding of heavy metals and harmful elements residues in Niuhuang Qingwei Pills, 49 samples from 18 manufactures were collected from 31 provinces in China. Risk assessment and control preparations were applied innovatively in evaluation of exogenous pollution in traditional Chinese Medicine. Determination methods for Pb, Cd, As, Hg and Cu were established by inductively coupled plasma mass spectrometry (ICP-MS). Based on the procedures including hazard identification, hazard characterization, exposure assessment and risk characterization, risk assessment was performed and residual limits for Pb, Cd, As, Hg and Cu in the drug were formulated. The results showed that the hazardous quotients (HQ) of the elements were decreased in the following order: Pb > As > Cu > Hg > Cd, and the total hazardous index (HI) of heavy metals and harmful elements in Niuhuang Qingwei Pills was above 1, implying health risk of the drug. Under the proposed limits, 5 elements in the control preparation as well as Cd and Cu in the samples were within the limits range, but the excess rates of Pb, As and Hg in the samples were 12%, 12% and 14%, respectively. For the first time, basic steps for risk assessment of Chinese patent medicine were established, which provided model and reference for risk assessment and limit formulation of other drugs.
In order to find the endogenous potential biomarkers of in vitro hepatic injury caused by sodium norcantharidin (NCTD-Na) and elucidate the mechanism of NCTD-Na-induced hepatic injury, ultra-high performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) was used for lipidomics detection. Multivariate statistical analysis was used to study the endogenous lipid metabolic changes of human normal liver LO2 cell injury after the treatment with NCTD-Na. The results showed that the half maximal inhibitory concentration (IC
50) of NCTD-Na was 0.034 mmol·L
−1. A total of 280 differential metabolites VIP > 2.0 and
P < 0.05 were found between the control group and the low-dose group. At the same time, a total of 273 differential metabolites with VIP > 2.0 and
P < 0.05 were found between the control group and the high-dose group. Cell metabolite profiles showed clear separation among control group, the low-dose group and the high-dose group, and 111 differential metabolites with VIP > 2.0,
P < 0.05, RSD < 30% and dose-dependent efficacy were found. It was found that most of the above differential metabolites were lipid metabolites after pretreatment and database search. A total of 32 potential biomarkers were identified, including three phosphatidylcholines (PCs), five lysophosphatidylcholines (Lyso PCs), three ceramides (Cers), one sphingomyelin (SM), one phosphatidylethanolamine (PE), ten lysophosphatidylethanolamines (LysoPEs), four diacylglycerols (DGs), one phosphatidic acid (PA), one lysophosphatidic acid (Lyso PA), one phosphatidyl glycerol (PG), one fatty acid hydroxy fatty acid (FAHFA) as well as one phosphatidylserine (PS). The changes in PCs, Cers, SM, PE and DGs were closely related to liver protection, DNA methylation and self-repair in hepatocytes, apoptosis, methylation and detoxification of carcinogens, as well as lipid peroxides production process. Also, they had an impact on the proliferation of hepatocytes, differentiation and gene transcription disorders. Cells stimulated by NCTD-Na could promote the production of PA as well as the synthesis and catabolism of FAHFA via a variety of ways. The levels of Lyso PCs, LysoPEs and Lyso PA were correlated with PCs, PE and PA. The outflow of PE and PS might be noticed during apoptosis, thus triggering phagocytosis.
