China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline.
Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study.
The journal is included in CA, JST and CSCD.
Honorary Editor-in-Chief Xiao Peigen Editor-in-Chief Wang Yongyan
Associate Editors Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board Cai Shaoqing, Chen Shilin
Pancreatic lipase (PL) inhibitors were firstly screened from
Prunella vulgaris with PL immobilized on carboxylic acid-terminated magnetic nanoparticles, and then these possible inhibitors were identified by LC-MS/MS and mixed reference alignment. Finally, their inhibitory effects and types on PL were tested by
p-nitrophenol method. The results showed that four PL inhibitors were screened out from
P.vulgaris and confirmed by LC-MS/MS and mixed reference alignment. The IC
50 values and inhibition types are as follows: caffeic acid [(252.3 ± 3.6) mg·L
−1, anti-competitive inhibition], rutin [(91.2 ± 1.6) mg·L
−1, competitive inhibition], hesperidin [(31.5 ± 4.4) mg·L
−1, competitive inhibition] and ursolic acid [(41.3 ± 2.2) mg·L
−1, competitive inhibition]. Their inhibitive types and abilities on PL were related to their molecular size, hydrophobicity and the number of hydrogen bond with PL triplet.
Intestinal absorption liquid was prepared by using everted intestinal sac method; meanwhile, its recipes were decomposed or restructured. Platelet aggregation activity was examined by biochemical tests and a microplate reader. One or more kinds of Chinese medicines which displayed inhibiting activity in Naoxintong Capsules were screened through separation and combination of prescription. The results showed that Naoxintong Capsules could inhibit ADP-induced platelet aggregation. Recipe decomposition and restructuring results showed that Salviae Miltiorrhizae Radix et Rhizoma, Paeoniae Radix Rubra, Cinnamomi Ramulus and Hirudo were the main effective medicines in inhibiting platelet aggregation. Furthermore, Cinnamomi Ramulus played a vital role in inhibiting activity among those four kinds of Chinese medicines. Coumarin derived from intestinal absorption liquid of Cinnamomi Ramulus had inhibiting activity in the range of 50–200 μmol·L
−1, and other ingredients such as cinnamyl alcohol and cinnamaldehyde also had inhibiting activities. In conclusion, Salviae Miltiorrhizae Radix et Rhizoma, Paeoniae Radix Rubra, Cinnamomi Ramulus and Hirudo are the main components for inhibiting ADP-induced platelet aggregation, and Cinnamomi Ramulus has the most strongest inhibiting activity in Naoxintong Capsules.
The model of drug-induced liver injury (DILI) induced by acetaminophen (APAP) in mice was established to investigate the anti-oxidation and anti-ferroptosis mechanisms of Fuzheng Yanggan Mixture on DILI. C57 BL/6 mice were randomly divided into five groups: control group, model group, positive group, and low and high-dose Fuzheng Yanggan Mixture groups (0.12 and 0.24 g·kg
−1) . Mice were intragastrically administration with Fuzheng Yanggan Mixture (0.12 and 0.24 g·kg
−1) once per day for 21 consecutive days, and at the same time, mice were weighted every day. The mice were injected intraperitoneally with 600 mg·kg
−1 of APAP to establish a mouse model of acute DILI after 16 h from the last administration of Fuzheng Yanggan Mixture. After 6 h from APAP challenge, the experimental animals were weighted and sacrificed to collect blood and liver tissue samples. And then, the effect of Fuzheng Yanggan Mixture on liver weight and the liver weight ratio of mice were examined; the content of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum and the level of malondialdehyde (MDA) , glutathione (GSH) and nicotinamide adenine dinucleotide phosphate (NADPH) in the liver tissue were measured. Prostaglandinendoperoxide synthase 2 (ptgs2) mRNA level in liver tissues was detected by Q-PCR, and protein expression levels of SLC7 A11 and GPX4 in liver tissues were detected by Western blot. Moreover, HE staining, immunohistochemical assay and TUNEL staining were used to observe pathological changes of the liver tissue sections. It is found that Fuzheng Yanggan Mixture could relieve APAP-induced liver enlargement and inhibit hepatic weight ratio increase. Compared with model group, the mice in Fuzheng Yanggan Mixture groups showed decreases in the content of ALT, AST and MDA, and increases in the content of GSH and NADPH. What is more, Fuzheng Yanggan Mixture could down-regulate ptgs2 mRNA level and up-regulate SLC7 A11 and GPX4 protein levels. All of the results lead to a conclusion that Fuzheng Yanggan Mixture plays a protective effect on DILI in mice, which may be associated with the inhibition of ferroptosis.
Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances. Total flavonoid (TFC), Phenolic acid (TPC), triterpenoid (TSC), polysaccharides (PC) and their antioxidant capacities (by the FRAP, DPPH and ABTS
+ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study. Then the multivariate statistical method was adopted to analyze the relationship between the multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica. The results showed that ethyl acetate fraction had relatively high concentration of TFC (0.61 ± 0.10) g·g
−1 DW, TPC (0.52 ± 0.09) g·g
−1 DW, and TSC (0.21 ± 0.03) g·g
−1 DW, with high scavenging capacity of DP-PH (3.1 ± 0.38) mmol·L
−1·g
−1 DW and FRAP (2.56 ± 0.35) mmol·L
−1·g
−1 DW. Hierarchical clustering analysis (HCA) and principal component analysis (PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the “differences” of different fractions. Canonical correlation analysis (CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities (
r = 0.970 0), and the first canonical variate had been reached. Moreover, ABTS
+ method showed a low response to the compositions of different fractions, so this method may not be suitable for evaluation of Prunellae Spica antioxidant capacities, while DPPH evaluation method was more suitable for TSC and TPC. The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.
A new device was designed to measure the adhesive force and predict whether Chinese herbal extracts would appear hot-melt sticking in spray drying process. Based on the physical property tester, the device was fixed with a camera, a temperature and humidity sensor probe and a set of equipment for hot air. This device could simulate the dry environment of spray dryer and analyze the variables of testing solutions' adhesiveness against drying time. To establish and validate the model in predicting hot-melt sticking in spray drying, data were collected by using data mining. The results of this study have proved that the device could be used to predict the hot-melt sticking in spray drying based on the adhesive force of materials (testing solutions). In addition, the study has also found that the adhesiveness of the hot-melt sticking materials gradually increased with the evaporation of water, while that of non-sticking materials was first increased and then rapidly decreased.
Ten compounds were isolated from the methanol extract of
Zanthoxylum nitidum through silica gel, Sephadex LH-20, RP-18 and HPLC chromatography techniques. Their structures were elucidated by the MS and NMR spectra as zanthonitidine B (
1), cyclo-(Leu-Leu-Leu-Leu-Ile) (
2), 6
S-10-
O-demethylbocconoline (
3), liriodenine (
4), isoplatydesmine (
5), 5,5′-dimethoxylariciresinol (
6), syringaresinol (
7), episyringaresinol (
8), marmesin (
9) and syringaldehyde (
10). Among them,
1 is a new alkaloid,
2 is a cyclopentapeptide isolated from plant kingdom for the first time, and
3 is from the genus
Zanthoxylum for the first time. Compounds
3 and
4 exhibit cytotoxicity against three human cancer cell lines HT29, A549 and MDA-MB-231 with IC
50 values of 27.37, 24.10, 33.58 μmol·L
−1 and 9.12, 6.05, 11.35 μmol·L
−1, respectively.
