China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline.
Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study.
The journal is included in CA, JST and CSCD.
Honorary Editor-in-Chief Xiao Peigen Editor-in-Chief Wang Yongyan
Associate Editors Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board Cai Shaoqing, Chen Shilin
Nineteen compounds were isolated from the water-soluble extract of the dry roots of
Paeonia lactiflora by using various chromatographic techniques. Their structures were identified by MS, NMR and other spectroscopic analysis as paeoniflorin (1), 4-
O-ethylpaeoniflorin (2), 2′-
O-benzoylpaeoniflorin (3), benzoylpaeoniflorin (4), 4′′-hydroxy-benzoyloxypaeoniflorin (5), moudanpioside C (6), 6′-
O-benzoyl-4′′-hydroxy-3′′-methoxy-paeoniflorin (7), paeoniflorin B (8), 6-
O-benzoylalbiflorin (9), secoisolariciresinol (10), (+)-lyoniresinol (11), dihyrodehydrodiconiferyl alcohol (12), (7
S, 8
S)-threo-7, 9, 9′-trihydroxy-3, 3′-dimethoxy-8-
O-4′-neolignan (13), (+)-neo-olivil (14), [(3
S)-5-methyl-2, 3-dihydro-1-benzofuran-3-yl] methanol (15), 5-hydroxy-3
S-hydroxymethyl-6-methyl-2, 3-dihydrobenzofuran (16),(+)-(
R)-2-hydroxy-1-(4-methoxyphenyl)-1-propan-1-one (17), (+)-(2
R)-1-(4-hydroxy-3-methoxyphenyl)-2-propanol (18), (+)-(4
S)-(2
E)-4-hydroxy-2-nonenoic acid (19). Compounds 15 and 18 are new natural products, while Compounds 10, 11, 13, 14, 17 and 19 are isolated from the genus
Paeonia for the first time.
This study focused on the protective effect of earthworm active ingredients (EWAs) on hepatocyte apoptosis induced by endoplasmic reticulum stress (ERS) in L-02 cells. The L-02 cells were cultured in vitro. The cell viability was measured with CCK-8, the apoptosis of L-02 cells was detected by flow cytometry, and the relevant protein and mRNA expression levels were detected by Western blot and qPCR. According to the findings, tunicamycin (TM) could obviously reduce the survival rate of L-02 cells in a time-dependent and dose-dependent manner. Compared with normal group, the apoptosis rate in model group was significantly increased (
P < 0.05 or
P < 0.01). The protein and mRNA expression levels of ERS-related signal molecules, such as GRP78, PERK, e LF2
α, ATF4, CHOP and Bax, were significantly up-regulated (
P < 0.05 or
P < 0.01), while Bcl-2 was significantly down-regulated (
P < 0.05 or
P < 0.01). After the administration with different concentrations of EWAs, compared with model group, EWAs could significantly increase the survival rate of L-02 hepatocyte and decrease the cell apoptosis rates. It could also reduce the protein and mRNA expressions of ERS-related signal molecules, such as GRP78, PERK, e LF2
α, ATF4, CHOP and Bax, in a dose-dependent manner (
P < 0.05 or
P < 0.01) and increased the protein and mRNA expression levels of Bcl-2 (
P < 0.05 or
P < 0.01). These results showed that EWAs had a significantly protective effect on the hepatocyte apoptosis induced by ERS in L-02 cells. Its mechanism may be related to the down-regulation of mRNA and protein expression levels of GRP78, PERK, ATF4, e LF2
α, CHOP and Bax, and the up-regulation, the alleviation of ERS, the up-regulation of Bcl-2, as well as the proliferation of impaired L-02 cells.
The Harpin protein Hpa1 can induce defense responses in plant. This study aimed at investigating the role of jasmonic acid (JA) signal pathway in the process of biosynthesis of secondary metabolite in
Sorbus aucuparia cell elicited by Hpa1 crude extract (Hpa1 CE). The results showed that Hpa1 CE could induce phytoalexin synthesis in
Sorbus aucuparia cells, with noraucuparin and its glycosides as the main metabolites. Meanwhile Hpa1 CE treatment resulted in increased methyl jasmonate (MeJA) production and large-scale synthesis of noraucuparin in cells. Combination of Hpa1 CE and salicylhydroxamic acid (SHAM, JA signaling inhibitor) caused the decreased MeJA and noraucuparin in the
Sorbus aucuparia cells as compared with those in Hpa1 CE group. Real-time PCR results indicated that Hpa1 CE treatment caused down-regulation of JAZ and up-regulation of
mcy2 in transcription level. Therefore Hpa1 CE elicited defense mechanism and JA signaling pathway involved in phytoalexin biosynthesis in
Sorbus aucuparia cells, which has elucidated the role of JA signal pathway in stress response from the perspective of secondary metabolism of plant.
The purpose of this study was to combine morphological, microscopic, UHPLC multiple-component assay and fingerprinting studies in order to evaluate the quality of Moutan Cortex (MC) systematically. The root system of
Paeonia suffruticosa was measured to compare the morphological variation and the chemical composition of different grades of MC was discussed according to previous studies. The difference between the main microscopic features of MC powder and the xylem powder is dramatic, the MC powder contains great amount of starch granules and clusters of calcium oxalate, while the xylem powder displays considerable vessels. Interestingly, the growth ring of
P.suffruticosa was first reported in the xylem of the root transection, this can help to determine the growth years of the plant. Moreover, through the assay of 16 component, MC produced in Tongling and Bozhou in Anhui province were compared, content of PGG in MC produced in Bozhou was significantly higher than MC produced in Tongling (
P < 0.01). MC with different growth years, MC with xylem and unprocessed MC and MC decoction pieces were compared respectively by combining the results of 16 compounds assay and fingerprinting. It is proposed that the quality evaluation standard includes the assay of paeoniflorin. Above all, the holistic quality difference can be evaluated more comprehensively by combining multiple analytical methods.
Polygonatum is a genus of the perennial herb family Liliaceae, with great potential in food, medicine and other field. In this study, genetic diversity and cluster structure analysis of 6 species in
Polygonatum. were investigated the molecular marker technique of simple sequence repeat (SSR). A total of 49 SSR makers were used to study genetic diversity and population structure within 60 germplasm resources which obtained from 38 counties and cities in 14 provinces of China. A total of 211 alleles were identified and the number of alleles ranged from 2 to 10, with an average of 4.306 1 alleles per SSR. The range of polymorphism information content (PIC) varied from 0.731 8 to 0.031 7, with the average of 0.309 6. The cluster analysis classified 60 germplasm resources into four defined groups at the genetic distance value of 0.26, among which most species with relatives were clustered into the same group. Extraordinarily, there were six germplasm resources clustered into other species, indicating that the classification of inter-genus and geographical distribution was crossed in
Polygonatum. The genetic diversity index of the four geographical populations from high to low was: Western region, Central China, Southern China, Eastern China. The population structure analysis, also indicating divided the entire collection into four groups, which was similar to the assignment pattern of cluster structure analysis. These results suggested that the
Polygonatum germplasm resources used in this study is rich in relatively high genetic diversity with large variation range, relatively fuzzy boundaries of species. It appeared the phenomenon that there is a difference decreases between the alternate leaf system and the rotation leaf system. The genetic diversity in the western region was higher than that in other regions, and the western region may be the origin center of the genus
Polygonatum.
