China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline.
Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study.
The journal is included in CA, JST and CSCD.
Honorary Editor-in-Chief Xiao Peigen Editor-in-Chief Wang Yongyan
Associate Editors Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board Cai Shaoqing, Chen Shilin
A total of 58 varieties in
Lonicera japonica from 20 producing areas were amplified by 22 pairs of SSR primers. Seven pairs of polymorphic primers were screened and their primers were used to establish DNA identity card and analyze genetic similarity. All the 58 varieties could be distinguished each other by the DNA identity card constituted by 7 pairs of core SSR primers. The genetic similarity coefficients of 58 varieties ranged from 0.366 7 to 0.916 7 by using Pop Gene32 (vesion1.32). Furthermore, all the varieties consistency were classified into four groups and constructed an evaluation table according to cluster analysis by an un-weighted pair-group average method with arithmetic mean. As expected, the results of cluster and evaluation table reflected 58 varieties relatives, which provide reference information for the selection of fine germplasm of
L.japonica and the theoretical basis for the study of Dao-di herbs.
Seven aromatic glycosides (1–7), including four phenylethanol glycosides, one phenylmethanol glycoside, one phenylpropane glycoside and one benzoside, were isolated from the methanolic extract of Uighur Medicine
Elaeagnus angustifolia flowers. Their structures were elucidated based on the analysis of spectroscopic data (1D, 2D NMR and HR-MS). Compound 1 was a new compound, named as angustifol A. Six known compounds were identified as 2-phenylethyl-
O-
β-D-glucopyranoside (2), salidroside (3), vanillic acid 4-
O-
β-D-glucopyranoside (4), vanilloloside (5), (
Z)-isoconiferin (6), 2-phenylethyl-6-
O-
α-L-arabinofuranosyl-
β-D-glucopyranoside (7). Compounds 2–7 were isolated from the genus Elaeagnus for the first time. The in vitro anti-inflammatory assays revealed that none of these compounds showed good COX inhibitory activities.
This paper was aimed to investigate the effects of emodin on oxidative stress and inflammatory response in rats with acute spinal cord injury (SCI), and to explore the protective mechanism of emodin on neurons after SCI. Rat SCI models were established using a modified Allen’s method. One hundred and ninety five Sprague-Dawley (SD) rats were randomly divided into sham operation group (group A), model group (group B), 20 mg·kg
−1 emodin treatment group (group C), and 40 mg·kg
−1 emodin treatment group (group D), and 80 mg·kg
−1 emodin treatment group (group E). Functional recovery was evaluated using the basso-beattie-bresnahan (BBB) scale and inclined plate test on the 3rd, 7th, 14th and 28th days. On the 7th day after SCI, Nissl bodies in the neurons were observed after toluidine blue staining. The changes of myelinated nerve fibers were observed by transmission electron microscopy. Nrf2, HO-1, NQO1, GFAP, NF-κB protein expression levels were detected by Western blot. The contents of TNF-
α, IL-1
β, and IL-6 were detected by ELISA. Immunofluorescence staining was performed to observe the expression of the GFAP, NG2 and ED-1. The BBB and inclined plate scores of group C, D and E were higher than those in group B on the 7th, 14th, and 28th days, and the difference was statistically significant (
P < 0.05). On the 7th day, Nissl bodies of groups B and C started to fuse, and the fusion of groups D and E was significantly alleviated than that in groups B and C. Transmission electron microscope showed that the changes of demyelination were obvious in groups B and C, those in groups D and E were significantly improved as compared with those in group B and C. Western blot showed that the differences in Nrf2, HO-1, GFAP, and NF-κB protein expression between groups C, D, E and group B were statistically significant, and the differences in NQO1 protein expression between groups D, E and group B were also statistically significant (
P < 0.05). ELISA showed that the differences in the contents of TNF-
α, IL-6 between groups C, D, E and group B were statistically significant, and the differences in IL-1
β contents between groups D, E and group B were also statistically significant (
P < 0.05); Immunofluorescence assay showed that the expression levels of GFAP and NG2 in groups C, D and E were higher than those in group B, and more obvious elevations were observed in groups D and E. The expression of ED-1 in groups C, D, and E were decreased significantly as compared with that in group B. Emodin has a protective effect on neurons after SCI. The mechanism may be related to the activation of Nrf2-ARE pathway, reduction in the expression of NF-κB, ED-1, TNF-
α, IL-1
β, and IL-6, and elevation in the expression of GFAP and NG2.
To evaluate the clinical efficacy of traditional Chinese medicine (TCM) spleen-tonification therapy in the treatment of atopic dermatitis (AD). CNKI, Wanfang knowledge service platform, VIP journal database, Chinese biomedical literature database (CBM), PubMed, the Cochrane Library and EMbase database from inception to December 2017 were searched for the randomized controlled trials (RCTs) on TCM spleen-tonification therapy in the treatment of AD. Literature selection and information extraction were conducted by two independent reviewers, and then the Cochrane recommended bias risk assessment method was used to evaluate the bias risk, and Review Manager 5.3 was used for the data analysis. Totally 37 clinical RCTs were included in this study, involving 2 973 patients. Analysis results showed that as compared with the western medicine, TCM spleen-tonification therapy had higher clinical effective rate, with statistically significant difference (OR = 4.05, 95% CI [3.27, 5.03],
P < 0.000 01); The improvement of score was more evident, including SCORAD score (WMD = −9.82, 95% CI [−13.31, −6.33],
P < 0.000 01), EA-SI score (WMD = −2.80, 95% CI [−3.54, −2.07],
P < 0.000 01) , and itching VAS score (WMD = −0.79, 95% CI [−1.10, −0.47],
P < 0.000 01); The improvement of serum biochemical levels was more evident, including interferon-γ (IFN-γ) (WMD = 1.75, 95% CI [1.14, 2.35],
P < 0.000 01), interleukin-4 (IL-4) (WMD = −3.15, 95% CI [−4.16, −2.15],
P < 0.000 01), and Eosinophil direct count (EOS) (WMD = −0.11, 95% CI [−0.20, −0.02],
P = 0.02); Recurrence rate was significantly reduced (OR = 0.36, 95% CI [0.21, 0.60],
P < 0.000 1). Trial-related adverse events were reported in 11 RCTs. Studies have shown that TCM spleen-tonification therapy had significantly higher clinical efficacy than western medicine in the treatment of AD. However, due to the publication bias and low quality bias of included RCTs in this study, more multicenter, high quality, large-sample, randomized double-blind controlled trials are needed to further verify the conclusion.
Hederagenin is an effective component of many medical plants such as Radix Clematidis, and it has a wide range of pharmacological activities, including anti-tumor, anti-inflammation, anti-depression, hepatoprotection, and anti-microbes. In order to obtain the efficient production of
Saccharonmyces cerevisiae cell factory for hederagenin, we successfully screened and obtained the P450 gene
MdMA02 that can catalyze the oxidation of the oleanolic acid at C-23 from the
Malus domestica, a plant belonging to the family of Rosaceae, with a plug-and-play platform for biosynthesis pathway analysis. The amino acid homology of oxidase gene
MdMA02 is only 32% of identified gene
CYP72
A68
v2. After being optimized through high-density fermentation, the strain BY-OA-
MdMA02 can produce hederagenin at 101 mg·L
−1, which is 337 times higher than that in shaking flask fermentation. This study provides a basis for further studies on promoting the biosynthetic pathway analysis of oleanane-type pentacyclic triterpenes and the construction of high-efficiency cell factories of hederagenin.
A new fatty acid methyl ester (
1) was isolated from an EtOH extract of
Fissistigma oldhamii. Its structure was elucidated by a combination of HR-ESI-MS, 1D NMR, 2D NMR, UV, IR spectroscopic data. The inhibitory effect of compound
1 on the proliferation of primary synoviocytes was evaluated. As a result, it showed inhibitory effect on the proliferation of synoviocytes, with an IC
50 value of 38.6 μmol·L
−1.
The paper-based analytical device (PAD) was applied in this study to analyze the antioxidant activity of Danhong Injection and its intermediates. First polycaprolactone was printed on the surface of a filter paper with a 3D printing device. The modified filter paper was then prepared using polycaprolactone and solid paraffin as the modifiers. The PAD was prepared after adding DPPH ethanol solution to the modified filter paper. Ascorbic acid solutions with different concentrations were used as the positive drug on PAD. After the occurrence of color reactions, the PAD was dried, and the data of color were collected by a cell phone. The color component G and gray scale were selected as the potential indices for measurement according to the values of determination coefficients, detection limits, and effective number of digits. Qualitative and quantitative analysis of Danhong Injection and the concentrate of aqueous extract were realized with the PAD. Because no significant differences were observed between the results obtained using the two potential indices, the average values of these two were used for analysis, and the antioxidant activity of Danhong Injection and the concentrate of aqueous extract was equivalent to ascorbic acid solutions of 3.7, 46 g·L
−1, respectively. The PAD method presented in this work can be a simple method to determine biological activities of Chinese medicines and their intermediates.
To explore the network pharmacological mechanisms of four anti-vitiligo Uyghur medicines based on the Phlegmatic temperament theory. First, the anti-vitiligo Uyghur medicine formulas based on Phlegmatic temperament theory were collected. The pharmacokinetic characteristics of main compounds in four anti-vitiligo Uyghur medicines were obtained by using admetSAR. The targets of active compounds were predicted via bSDTNBI (balanced substructure-drug-target network-based inference model) method. Then, biological process (BP) and molecular function (MF) enrichment analysis of targets were analyzed via DAVID database. Constructing anti-vitiligo Uyghur medicine formula-Uyghur medicines network model (FMI network) and Uyghur medicines-active compounds-targets BP-Hilit network model (MCTBHI network), we utilized closeness centrality to analyze key Uyghur medicines, active compounds, key targets as well as Hilit. Finally, the in vitro melanin production model of C57BL/6 mice was used to verify the ability of the active compounds to improve melanogenesis. The results showed that
Psoralea corylifolia,
Vernonia anthelmintica, Flos Caryophylli and
Anacyclus pyrethrum were the key Uyghur medicines in the FMI network. There were 22 active compounds with a relatively higher bioavailability interacted with 58 therapeutic targets. These active compounds were mainly composed of coumarins and flavonoids. In the MCTBHI network, the MF of 58 therapeutic targets was related to steroid hormone receptor activity, heme binding and enzyme binding function. Classification of the Hilit according to the BP of 58 therapeutic targets, the first place was the blood, followed by the lymph, the cerebrospinal fluid and digestive juice. It was found that the expression of some targets located in the skin was closed to the heart muscle, lymph node, spleen, cerebral cortex and so on, which were the main places for Hilit. In particular, ESR1, PTGS2, PPARA, PPARG, PTGS1 and CA2 were regulated by the flavonoids (kaempferide and isorhamnetin). The in vitro melanin production model showed that kaempferide and isorhamnetin could promote the melanin production in C57BL/6 mice ear skin. Based on admetSAR and bSDTNBI, we used network pharmacological method to construct a systematic means of studying anti-vitiligo Uyghur medicines, providing clues for the further study of the modern molecular mechanisms of Phlegmatic temperament.
