China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline.
Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study.
The journal is included in CA, JST and CSCD.
Honorary Editor-in-Chief Xiao Peigen Editor-in-Chief Wang Yongyan
Associate Editors Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board Cai Shaoqing, Chen Shilin
In order to clarify the chemical constituents of
Cistanche deserticola cultured in Tarim desert, a systematically phytochemical investigation was carried out. The chemical constituents were isolated by column chromatography, such as silica gel, Sephadex LH-20, MCI gel, ODS and semi-preparative HPLC, and their structures were determined on the basis of MS, NMR spectroscopic analysis, and comparison with literature data. Four compounds were isolated from the 85% ethanol extract of the stems of C.
deserticola cultured in Tarim desert. Their structures were identified as
cis-tubuloside (1),
cis-cistanoside (2),
cis-cistanoside J (3), and
cis-isocistanoside C (4). Compounds 1–4 were four new
cis-phenylethanoid glycosides. Herein, we firstly report the
1H,
13C-NMR data of the new compounds (1-4) for the first time. This study will provide the scientific evidence for comprehensively analyzing the chemical constituents of
C. deserticola cultured in Tarim desert.
This paper aimed to investigate the hypoglycemic effect of jatrorrhizine on insulin-resistance (IR)-3T3-L1 adipocytes and the relative mechanism. The 3T3-L1 preadipocytes were used to induce mature adipocytes and then the stable IR model was established with 1 μmol·L
−1 dexamethasone. The adipocytes were divided into normal group, IR group, rosiglitazone positive group and jatrorrhizine group (0.5, 1, 5, 10, 20 μmol·L
−1). After different time (12, 24, 30, 36, 48 h) of treatment, glucose content of 3T3-L1 adipocytes was detected with the glucose oxidase peroxidase method and TG content was measured with the glycerol phosphate oxidase method, whereas cell viability was detected by CCK-8 assay. Furthermore, the protein expression levels of insulin receptor substrate 2 (IRS2), phosphinositide-3-kinase regulatory subunit 1 (PI3KR1), phosphorylated protein B [p-AKT(Ser473)], phosph-AMP-activated protein [p-AMPK(Thr172)], and glucose transporter type 4/1/2 (GLUT4/1/2) were detected by Western blot assay. The results showed that as compared with the normal group, the glucose consumption was significantly decreased in IR group (
P < 0.01), whereas 0.5, 1, 5, 10, 20 μmol·L
−1 jatrorrhizine and rosiglitazone groups elevated the glucose consumption of IR-3T3-L1 cells (
P < 0.01) after the 36 h and 48 h administration as compared with IR group. The optimal administration time was 48 h for jatrorrhizine. The 1, 5, 10, 20 μmol·L
−1 jatrorrhizine decreased the TG content in 3T3-L1 adipocytes after 48 h administration (
P < 0.05). The protein expression levels of IRS2, PI3KR1, p-AKT (Ser473), p-AMPK (Thr172), GLUT4/1/2 were significantly up-regulated by different concentration of jatrorrhizine and rosiglitazone (
P < 0.01). The results showed that jatrorrhizine increased glucose uptake with elevated glucose consumption, whereas reduced intracellular TG content in IR-3T3-L1 adipocytes. Moreover, it intervened classic insulin signal pathway IRS2/PI3KR1/p-AKT/GLUT4 and increase AMPK protein phosphorylation level for the activation of GLUT1/4 for insulin sensibility. Thus, jatrorrhizine could effectively regulate the GLUTs with multiple manners for hypoglycemic effect.
To assess the clinical efficacy of Chinese medical injection (Chinese medical injection) for heart failure by using network Meta-analysis method. The relative randomized controlled trials (RCTs) of CMI for heart failure were retrieved from China National Knowledge Infrastructure (CNKI), WanFang Database, Chinese Biomedical Literature Database (Sino Med), Pub Med, Cochrane Library and EMbase in July 2017. RCTs on the comparison of two kinds of CMIs for heart failure were included. Two researchers independently completed the literature screening, data extraction and quality evaluation according to the pre-determined inclusion and exclusion criteria, and the results were crossed checked. The data were analyzed by Win Bugs, and STATA software was used for plotting. Finally, 13 RCTs were included, involving 5 kinds of CMIs and 1 538 patients. According to the quality evaluation, the appropriate random dividing methods were reported in only two RCTs, double-blindness was used in only one RCT, and even none of the RCTs mentioned allocation concealment. Network Meta-analysis showed that Shenmai injection had the greatest effect in the clinical efficacy for patients with heart failure, which was followed by Shenfu Injection. However, Shenfu Injection was most effective in improving the patients’ left ventricular ejection fraction (LVEF), which was followed by Shenmai Injection. Therefore, Shenfu Injection and Shenmai Injection had certain advantages in treating heart failure. However, due to the limited sample size and the poor literature quality, more studies were required to verify the strength of evidence. We suggest that further studies shall pay more attention to the improvement of the methodological quality, increase the follow-up period, and strengthen the observation of cardiovascular end points.
The isotope ratios of Sr isotope (Sr) and light elements (C/H/O/N) in
Dendrobium officinale from different producing areas (Shaoguan, Guangdong; Yulin, Guangxi; Shibing, Guizhou; Wenshan, Yunnan and Zhejiang Province) were determined with thermal ionization mass spectrometry (TI-MS) and isotope ratio mass spectrometry (IRMS). The difference of the stable isotope ratios in
D. officinale was obtained by the variance analysis and the correlation analysis, and pattern recognition techniques with principal component analysis (PCA) were used to classify the geographical origins of
D. officinale from different producing areas. The isotope ratios of Sr can be used to identify
D. officinale in Zhejiang Province, and the isotope ratios of light elements showed the difference in different producing areas.
δD and
δ18O in samples were influenced by the microenvironment effect. A positive correlation was found between
δD and
δ18O. The PCA was used to discern the samples of
D. officinale from different producing areas based on detection technology of stable isotope ratios. These results revealed that it was possible and feasible to classify the geographical origin of
D. officinale with the method of determination of isotopes, and provided a new method to identificate origin information of Chinese medicinal materials.
To investigate the chemical compositions of “antler powder” and “antler slice”, two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia. With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study. The results showed that as compared with the antler powder by directly “chopping into pieces, and grinding into fine powder”, the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol. While the contents of fatty acids and mineral elements were not decreased in these three methods. Polysaccharide, crude protein, amino acids, and nucleosides contents in “antler slices” were less than those in “antler powder”, most notably in polysaccharides and nucleosides. According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.
In this study, RACE technology was employed to isolate the full length cDNA of DoHT1 in
Dendrobium officinale, followed by bioinformatics analysis of the sequence characteristics. And the expression pattern of the gene was also analyzed by quantitative PCR. The full length cDNA of DoHT1 was 1 586 bp in length, containing a 1 536 bp ORF, which encoded a 511-aa protein with molecular weight of 56.18 kD and isoelectric point of 9.08. The deduced DoHT1 protein had the major facilitator superfamily conserved domain (22–483), SUGAR_TRANSPORT_1 (139–164), and SUGAR_TRANSPORT_2 (338–355), typical for sugar transporter; DoHT1, without a single peptide had 11 transmembrane regions, and was predicted to locate in the plasma membrane; DoHT1 had high identities (54.7%–80.7%) with
HTs proteins from various plants. DoHT1 belonged to the MST (monosaccharide transporter) group of the evolutionary tree, and was closely related to the
Phalaenopsis equestris. DoHT1 was differentially expressed in the three included organs. The transcripts were significantly the most abundant in the leaves with 19.36 fold than roots, then 1.82 fold in the stems than the roots. The identification and molecular characterization of the full length DoHT1 will be essential for further function study of the gene during the regulation of sugar metabolism of
D. officinale.
To evaluate the effectiveness and safety of Xinling Wan on patients with stable angina pectoris, a randomized, double-blinded, placebo parallel-controlled, multicenter clinical trial was conducted. A total of 232 subjects were enrolled and randomly divided into experiment group and placebo group. Patients in the experiment group were treated with Xinling Wan (two pills each time, three times daily) for four weeks, and those in the placebo group were treated with placebo. The effectiveness evaluation showed that Xinling Wan could significantly increase the total duration of treadmill exercise among patients with stable angina pectoris. FAS analysis showed that the difference values of the total exercise duration in the experiment group and placebo group were (72.11 ± 139.32) s and (31.25 ± 108.32) s. Xinling Wan could remarkably increase the total effective rate of angina pectoris symptom score, and the analysis showed that the total effective rates were 78.95% and 42.61% in the experiment group and placebo group. The reduction of nitroglycerin dose was (2.45 ± 2.41) tablets in experiment group and (0.50 ± 2.24) tablets in placebo group on the basis of FAS analysis. The decrease of symptom integral was (4.68 ± 3.49) in experiment group and (3.19 ± 3.31) in placebo group based on FAS analysis. Besides, Xinling Wan could decrease the weekly attack time and the duration of angina pectoris. PPS analysis results were similar to those of FAS analysis. In conclusion, Xinling Wan has an obvious therapeutic effect in treating stable angina pectoris, with a good safety and a low incidence of adverse event and adverse reaction in the experiment group.
